Mitochondrial COI gene as a tool in the taxonomy of mosquitoes Culex subgenus Melanoconion

Gutiérrez, Carolina Torres; Bergo, Eduardo Sterlino; Emerson, Kevin J.; Oliveira, Tatiane Marques Porangaba de; Greni, Susan Elaine; Sallum, Maria Anice Mureb

doi:10.1016/j.actatropica.2016.09.007

Cited by 28 publications

(29 citation statements)

References 51 publications

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“…Recent studies indicate that it is necessary to keep identifying mosquito species in different regions of the world through the use of traditional taxonomy and DNA sequences, to achieve a better estimation of mosquito biodiversity (Ashfaq et al, ; Azari‐Hamidian et al, ; Batovska, Blacket, Brown, & Lynch, ; Cywinska, Hunter, & Hebert, ; Rozo‐López & Mengual, ; Versteirt et al, ; Wang et al, ; Weeraratne, Surendran, & Parakrama Karunaratne, ). The combined use of DNA and morphology allowed to analyze a greater number of species worldwide and to discover cryptic speciation in taxa of medical importance such as culicides (Ashfaq et al, ; Hebert & Gregory, ; Laurito, Oliveira, Almirón, & Sallum, ; Torres‐Gutiérrez et al, ; Wang et al, ). Though, given the diversity of mosquitoes, it is possible to affirm that integrative taxonomy in this group is at its early stages (Beebe, ).…”

Section: Introductionmentioning

confidence: 99%

“…allowed to analyze a greater number of species worldwide and to discover cryptic speciation in taxa of medical importance such as culicides (Ashfaq et al, 2014;Hebert & Gregory, 2005;Laurito, Oliveira, Almirón, & Sallum, 2013;Torres-Gutiérrez et al, 2016;Wang et al, 2012). Though, given the diversity of mosquitoes, it is possible to affirm that integrative taxonomy in this group is at its early stages (Beebe, 2018).…”

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confidence: 99%

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DNA barcodes and evidence of cryptic diversity of anthropophagous mosquitoes in Quintana Roo, Mexico

Chan-Chablé

Martínez‐Arce

Mis-Ávila³

et al. 2019

Ecology and Evolution

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Culicidae mosquitoes are potential vectors of pathogens that affect human health. The correct species identification, as well as the discovery and description of cryptic species, is important in public health for the control and management of specific vectors. In the present study, the diversity of anthropophagous mosquitoes in Quintana Roo, at the border between Mexico and Belize, was evaluated using morphological and molecular data (COI‐DNA Barcoding). A total of 1,413 adult female specimens were collected, belonging to eight genera and 31 morphospecies. Most species formed well‐supported clades. Intraspecific Kimura 2 parameters (K2P) distance average was 0.75%, and a maximum distance of 4.40% was observed for Anopheles crucians s.l. ABGD method identified 28 entities, while 32 entities were identified with the BIN system. In Culex interrogator and Culex nigripalpus a low interspecific genetic distance of 0.1% was observed. One undescribed species belonging to the genus Aedes ( Aedes n. sp.) was discovered, but no clear genetic divergence was found between this species and the closely related species Aedes angustivittatus . An intraspecific K2P distance greater than 2.7% was observed in Aedes serratus (3.9%), Anopheles crucians s.l. (4.4%), Culex taeniopus (3.7%), Haemagogus equinus (3.9%), Culex erraticus (5.0%), Psorophora ferox (4.5%), and in Anopheles apicimacula (8.10%); therefore, evidences of cryptic diversity are shown in these species. This study showed that DNA barcodes offer a reliable framework for mosquito species identification in Quintana Roo, except for some closely related species for which it is recommended to use additional nuclear genetic markers such as ITS2, in order to resolve these small discrepancies.

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Section: Introductionmentioning

confidence: 99%

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confidence: 99%

DNA barcodes and evidence of cryptic diversity of anthropophagous mosquitoes in Quintana Roo, Mexico

Chan-Chablé

Martínez‐Arce

Mis-Ávila³

et al. 2019

Ecology and Evolution

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“…For mosquitoes, the suitability of the COI gene for species identification was first tested on 37 species occurring in Canada [16]. Since then, barcoding has been used for mosquito species in many parts of the world, including India [17], Iran [18], China [19], Argentina [20], Ecuador [21; 22], Pakistan [23], Singapore [24], Belgium [25], Colombia [26] and Brazil [27]. In most cases, these studies show a high correspondence between morphological species delimitation and mtDNA barcode clusters, but others point out the inability of the method to separate some closely related species distinguished by traditional taxonomy [20].…”

Section: Introductionmentioning

confidence: 99%

DNA reference libraries of French Guianese mosquitoes for barcoding and metabarcoding

et al. 2017

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The mosquito family (Diptera: Culicidae) constitutes the most medically important group of arthropods because certain species are vectors of human pathogens. In some parts of the world, the diversity is so high that the accurate delimitation and/or identification of species is challenging. A DNA-based identification system for all animals has been proposed, the so-called DNA barcoding approach. In this study, our objectives were (i) to establish DNA barcode libraries for the mosquitoes of French Guiana based on the COI and the 16S markers, (ii) to compare distance-based and tree-based methods of species delimitation to traditional taxonomy, and (iii) to evaluate the accuracy of each marker in identifying specimens. A total of 266 specimens belonging to 75 morphologically identified species or morphospecies were analyzed allowing us to delimit 86 DNA clusters with only 21 of them already present in the BOLD database. We thus provide a substantial contribution to the global mosquito barcoding initiative. Our results confirm that DNA barcodes can be successfully used to delimit and identify mosquito species with only a few cases where the marker could not distinguish closely related species. Our results also validate the presence of new species identified based on morphology, plus potential cases of cryptic species. We found that both COI and 16S markers performed very well, with successful identifications at the species level of up to 98% for COI and 97% for 16S when compared to traditional taxonomy. This shows great potential for the use of metabarcoding for vector monitoring and eco-epidemiological studies.

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“…In contrast, male genitalia have remarkable structures being the main identification resource (Consoli & Lourenço-de-Oliveira, 1994;Harbach, 2011). Furthermore, several species complexes of Culex and Melanoconion subgenera can only be correctly identified with complementary information about adult females, their cibarial armature (Williams & Savage, 2009), and larval and pupal characters from exuviae (Demari-Silva, B., Vesgueiro, F. T., Sallum, M. A., Marelli, M. T., 2011;Torres-Gutierrez et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…The obstacles in morphological taxonomy can be magnified if the specimens are captured and manipulated in the field -in most cases causing damages to the specimens structure and organ loss -which hinder reliable identification by taxonomists (Torres- Gutierrez et al, 2016). In this context, molecular identification approaches allow to determine and discover new species through the analysis of a small segment of the genome, representing an efficient tool that would facilitate the diagnosis of biological diversity ( Hebert, P. D. N., Cywinska, A., Ball, S. L., deWaard, J. R., 2003).…”

Section: Introductionmentioning

confidence: 99%

Identificação de algumas espécies amazônicas de Culex (Culex) e Culex (Melanoconion) por meio de morfotipagem e barcoding

et al. 2019

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Culex spp. mosquitoes have idiosyncratic characteristics and its low variability makes difficult their identification. The aim of our study was to analyze the 5' region of the cytochrome oxidase subunit I gene (coI) for the taxonomic identification of Culex species which were previously morphotyped and diagnosed in Culex and Melanoconion subgenera at the field conditions. Ten specimen sequences were analyzed by the Automatic Barcode Gap Discovery (ABGD). All sequences showed 94-99% identity when compared to other Culex species sequences available from GenBank. Five initial partitions supported 80-88 species groups. Among them, eight sets contained the specimens of the present study. Of the 10 mosquito sequences, five did not form any consistent cluster, and the remaining showed some consistency in the taxonomic diagnosis at the field conditions. Our results suggest that some coI gene sequences of specimens may belong to species of the subgenus Melanoconion, whose 5' coI sequence is unknown or unpublished in GenBank. Palavras-chave: ABGD, coI gene, HP trap, Neighbor-Joining method, Taxonomic Diagnosis. ResumoOs mosquitos Culex spp. apresentam características idiossincráticas e sua baixa variabilidade dificulta sua identificação. O objetivo do nosso estudo foi analisar a região 5 'do gene da subunidade I do citocromo oxidase (coI) para a identificação taxonômica de espécies de Culex que foram previamente diagnosticadas em subgêneros Culex e Melanoconion em condições de campo. Dez sequências de espécimes foram analisadas pelo Automatic Barcode Gap Discovery (ABGD). Todas as sequências apresentaram 94-99% de identidade quando comparadas com outras sequências de espécies de Culex disponíveis no GenBank. Cinco partições iniciais suportaram 80-88 grupos de espécies. Entre eles, oito conjuntos continham os espécimes do presente estudo. Das 10 sequências de mosquitos, cinco não formaram nenhum cluster consistente, e as demais apresentaram alguma consistência no diagnóstico taxonômico nas condições de campo. Nossos resultados sugerem que algumas sequências do gene coI de espécimes podem pertencer a espécies do subgênero Melanoconion, cuja sequência 5' coI é desconhecida ou inédita no GenBank.

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Mitochondrial COI gene as a tool in the taxonomy of mosquitoes Culex subgenus Melanoconion

Cited by 28 publications

References 51 publications

DNA barcodes and evidence of cryptic diversity of anthropophagous mosquitoes in Quintana Roo, Mexico

DNA barcodes and evidence of cryptic diversity of anthropophagous mosquitoes in Quintana Roo, Mexico

DNA reference libraries of French Guianese mosquitoes for barcoding and metabarcoding

Identificação de algumas espécies amazônicas de Culex (Culex) e Culex (Melanoconion) por meio de morfotipagem e barcoding

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