Mitochondrial Contact Site and Cristae Organization System and F
            <sub>1</sub>
            F
            <sub>O</sub>
            -ATP Synthase Crosstalk Is a Fundamental Property of Mitochondrial Cristae

Cadena, Lawrence Rudy; Gahura, Ondřej; Panicucci, Brian; Zı́ková, Alena; Hashimi, Hassan

doi:10.1128/msphere.00327-21

Cited by 15 publications

(14 citation statements)

References 64 publications

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“…Interestingly, Tb927.8.3070 has not been detected in previous pull-down MS analyses of the T. brucei F 1 F o complexes ( 33 , 61 ). This could be explained by complex disruption during extraction and/or by its low molecular mass and having few tryptic cleavage sites, which may make detection by proteomic methods difficult ( 62 ).…”

Section: Discussionmentioning

confidence: 85%

“…However, should this be the case, no similarity with subunit b from Euglena or from other organisms would be expected. Interestingly, Tb927.8.3070 has not been detected in previous pull-down MS analyses of the T. brucei F1Fo complexes (33,61). This could be explained by complex disruption during extraction and/or by the low molecular mass and the few tryptic cleavage sites of subunit b which may make detection by proteomic methods difficult (62).…”

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 94%

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Characterization of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei

Dewar

Oeljeklaus

Wenger

et al. 2022

Journal of Biological Chemistry

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Section: Discussionmentioning

confidence: 85%

Section: J O U R N a L P R E -P R O O Fmentioning

confidence: 94%

Characterization of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei

Dewar

Oeljeklaus

Wenger

et al. 2022

Journal of Biological Chemistry

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“…5d). Furthermore, kinetoplastid parasites and mammals share conserved GXXXG motifs in subunit- e 34 and - g (Extended Data Fig. 8), which allow close interaction of their transmembrane helices (Fig.…”

Section: Resultsmentioning

confidence: 99%

An ancestral interaction module promotes oligomerization in divergent mitochondrial ATP synthases

Gahura

Muhleip

Hierro-Yap

et al. 2021

Preprint

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Mitochondrial ATP synthase forms stable dimers arranged into oligomeric assemblies that generate the inner-membrane curvature essential for efficient energy conversion. Here, we report cryo EM structures of the intact ATP synthase dimer from trypanosomes in 10 different rotational states. The model consists of 25 subunits, including 11 lineage-specific, as well as 36 lipids. The rotary mechanism is influenced by the divergent peripheral stalk, conferring a greater conformational flexibility. Proton transfer in the lumenal half-channel occurs via a chain of five ordered water molecules. The dimerization interface is formed by subunit-g that is critical for interactions but not for the catalytic activity. Although overall dimer architecture varies among eukaryotes, we find that subunit-g and -e form a common ancestral oligomerisation motif, which is shared between the trypanosomal and mammalian lineages. Therefore, our data defines the subunit-g/e module as a structural component determining ATP synthase oligomeric assemblies.

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“…The remaining beads from triplicate APs of Mic60-6xHis, Orf52-6xHis and the mock controls were trypsinized and analyzed by LC-MS/MS. Protein enrichment in bait-APs in comparison to the mock control was quantified using label-free quantification as previously described (Cadena et al 2021). In each AP, 497 high-confidence proteins were found (Dataset 2) based on mean Andromeda confidence scores >100 in three biological replicates (Cox et al 2011).…”

Section: Resultsmentioning

confidence: 99%

The development of intracytoplasmic membranes in alphaproteobacteria involves the conserved mitochondrial crista-developing protein Mic60

Muñoz-Gómez

Cadena

Gardiner

et al. 2022

Preprint

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Mitochondrial cristae expand the surface area of respiratory membranes and ultimately allow for the evolutionary scaling of respiration with cell volume across eukaryotes. The discovery of Mic60 homologs among alphaproteobacteria, the closest extant relatives of mitochondria, suggested that cristae might have evolved from bacterial intracytoplasmic membranes (ICMs). Here, we investigated the predicted structure and function of alphaproteobacterial Mic60, and a protein encoded by an adjacent gene Orf52, in two distantly related purple alphaproteobacteria, Rhodobacter sphaeroides and Rhodopseudomonas palustris. In addition, we assessed the potential physical interactors of Mic60 and Orf52 in R. sphaeroides. We show that the three alpha-helices of mitochondrial Mic60's mitofilin domain, as well as its adjacent membrane-binding amphipathic helix, are present in alphaproteobacterial Mic60. The disruption of Mic60 and Orf52 caused photoheterotrophic growth defects, which are most severe under low light conditions, and both their disruption and overexpression lead to enlarged ICMs in both studied alphaproteobacteria. We also found that alphaproteobacterial Mic60 physically interacts with BamA, the homolog of Sam50, one of the main physical interactors of eukaryotic Mic60. This interaction, responsible for making contact sites at mitochondrial envelopes, has been conserved in modern alphaproteobacteria despite more than a billion years of evolutionary divergence. Our results suggest a role for Mic60 in photosynthetic ICM development and contact site formation at alphaproteobacterial envelopes. Overall, we provide support for the hypothesis that mitochondrial cristae evolved from alphaproteobacterial ICMs, and therefore have improved our understanding of the nature of the mitochondrial ancestor.

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Mitochondrial Contact Site and Cristae Organization System and F ₁ F _O -ATP Synthase Crosstalk Is a Fundamental Property of Mitochondrial Cristae

Abstract: Mitochondria have undergone profound diversification in separate lineages that have radiated since the last common ancestor of eukaryotes some eons ago. Most eukaryotes are unicellular protists, including etiological agents of infectious diseases, like Trypanosoma brucei .

Cited by 15 publications

References 64 publications

Characterization of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei

Characterization of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei

An ancestral interaction module promotes oligomerization in divergent mitochondrial ATP synthases

The development of intracytoplasmic membranes in alphaproteobacteria involves the conserved mitochondrial crista-developing protein Mic60

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Mitochondrial Contact Site and Cristae Organization System and F 1 F O -ATP Synthase Crosstalk Is a Fundamental Property of Mitochondrial Cristae

Abstract: Mitochondria have undergone profound diversification in separate lineages that have radiated since the last common ancestor of eukaryotes some eons ago. Most eukaryotes are unicellular protists, including etiological agents of infectious diseases, like Trypanosoma brucei .

Cited by 15 publications

References 64 publications

Characterization of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei

Characterization of a highly diverged mitochondrial ATP synthase Fo subunit in Trypanosoma brucei

An ancestral interaction module promotes oligomerization in divergent mitochondrial ATP synthases

The development of intracytoplasmic membranes in alphaproteobacteria involves the conserved mitochondrial crista-developing protein Mic60

Contact Info

Product

Resources

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Mitochondrial Contact Site and Cristae Organization System and F ₁ F _O -ATP Synthase Crosstalk Is a Fundamental Property of Mitochondrial Cristae