Mitochondrial control of cell surface characteristics in Saccharomyces cerevisiae

Evans, Ivor H.; Diala, Edward S.; Earl, Alison J.; Wilkie, D.

doi:10.1016/0005-2736(80)90302-8

Cited by 39 publications

(12 citation statements)

References 7 publications

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“…IFM1, SMP2 and COX11 are all nuclear petite genes [33–35] with cell surface phenotypes. Earlier literature on this theme has been overlooked [36,37]. The double cell wall/mitochondria localization of two members of the SUN family Uth1p and Sun4p, with phenotypes associated to cytokinesis for Sun4p and to mitochondrial biogenesis for Uth1p further highlights the existence of some regulatory link between mitochondrial function and the cell surface.…”

Section: Discussionmentioning

confidence: 99%

Dual cell wall/mitochondria localization of the âSUNâ family proteins

Velours

Boucheron

Manon

et al. 2002

FEMS Microbiology Letters

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The Saccharomyces cerevisiae SUN family gene products, namely Sim1p, Uth1p, Nca3p and Sun4p, show a high degree of homology among themselves and are closely related to beta-glucosidase of Candida wickerhamii; however, these proteins do not bear such an activity. Dithiothreitol-treatment of intact cells induces the release of Uth1p, Sun4p and Sim1p from the cell wall. These highly glycosylated proteins are thus non-covalently bound to the cell wall. Two of them, Uth1p and Sun4p, have also been found in mitochondria. Sub-localization experiments show that Uth1p is inserted in the outer mitochondrial membrane and that Sun4p is preferentially a matrix protein. The physiological significance of this double localization is discussed in relation to the roles of these proteins in different cellular processes, namely mitochondrial biogenesis and cell septation.

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Section: Discussionmentioning

confidence: 99%

Dual cell wall/mitochondria localization of the âSUNâ family proteins

Velours

Boucheron

Manon

et al. 2002

FEMS Microbiology Letters

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show abstract

“…IFM1, SMP2 and COX11 are all nuclear petite genes [33^35] with cell surface phenotypes. Earlier literature on this theme has been overlooked [36,37]. The double cell wall/mitochondria localization of two members of the SUN family Uth1p and Sun4p, with phenotypes associated to cytokinesis for Sun4p and to mitochondrial biogenesis for Uth1p further Mitochondria from vuth1-galUTH1 were isolated and puri¢ed on a density sucrose gradient.…”

Section: Discussionmentioning

confidence: 99%

Dual cell wall/mitochondria localization of the 'SUN' family proteins

Velours

2002

FEMS Microbiology Letters

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The Saccharomyces cerevisiae SUN family gene products, namely Sim1p, Uth1p, Nca3p and Sun4p, show a high degree of homology among themselves and are closely related to L-glucosidase of Candida wickerhamii; however, these proteins do not bear such an activity. Dithiothreitol-treatment of intact cells induces the release of Uth1p, Sun4p and Sim1p from the cell wall. These highly glycosylated proteins are thus non-covalently bound to the cell wall. Two of them, Uth1p and Sun4p, have also been found in mitochondria. Sub-localization experiments show that Uth1p is inserted in the outer mitochondrial membrane and that Sun4p is preferentially a matrix protein. The physiological significance of this double localization is discussed in relation to the roles of these proteins in different cellular processes, namely mitochondrial biogenesis and cell septation. ß

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“…Of these flocculating mutants, rmr1, rgr1 (47) (16,25) or inappropriate respiratory activity. In addition to the two RMR genes characterized here, we have described the CYB2 regulation in HXK2 and SSN6 null mutants.…”

Section: Discussionmentioning

confidence: 99%

“…Addition of 15 mM EDTA to chelate divalent cations eliminated the flocculation, which is, therefore, probably calcium dependent. BH1-10A [rho 0 ] cells isolated after treatment with ethidium bromide maintained the flocculating phenotype, indicating that the mitochondrial genome is not necessary for this type of flocculation as in other nuclear backgrounds (16,25). BH1-10A grown anaerobically in YP 8% dextrose also flocculated, indicating that oxygen or heme regulatory effects do not influence this phenotype.…”

Section: Mutant Selectionmentioning

confidence: 99%

Regulation of nuclear genes encoding mitochondrial proteins in Saccharomyces cerevisiae

1995

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Selection for mutants which release glucose repression of the CYB2 gene was used to identify genes which regulate repression of mitochondrial biogenesis. We have identified two of these as the previously described GRR1/CAT80 and ROX3 genes. Mutations in these genes not only release glucose repression of CYB2 but also generally release respiration of the mutants from glucose repression. In addition, both mutants are partially defective in CYB2 expression when grown on nonfermentable carbon sources, indicating a positive regulatory role as well. ROX3 was cloned by complementation of a glucose-inducible flocculating phenotype of an amber mutant and has been mapped as a new leftmost marker on chromosome 2. The ROX3 mutant has only a modest defect in glucose repression of GAL1 but is substantially compromised in galactose induction of GAL1 expression. This mutant also has increased SUC2 expression on nonrepressing carbon sources. We have also characterized the regulation of CYB2 in strains carrying null mutations in two other glucose repression genes, HXK2 and SSN6, and show that HXK2 is a negative regulator of CYB2, whereas SSN6 appears to be a positive effector of CYB2 expression.When Saccharomyces cerevisiae strains are grown on glucose, they down regulate the biogenesis of mitochondria such that the volume occupied by mitochondria is decreased from 12 to 14% of the total cellular volume under derepressing conditions to 3 to 4% under repressing conditions. The glucose-repressed mitochondria also have poorly differentiated inner membrane cristae (51). Although this effect of glucose on yeast mitochondria was discovered over 40 years ago (14), surprisingly little is known about the mechanism of glucose repression of mitochondrial biogenesis.Much of what we know about glucose repression is from studies of the SUC and GAL genes, which are involved in the regulation of sucrose and galactose metabolism, respectively (27). Some genes which regulate repression of sucrose and galactose catabolic enzymes also regulate at least some mitochondrial enzymes. For example, mutations in HXK2, which encodes one of the hexokinases, release repression of mitochondrial cytochrome c reductase and cytochrome c oxidase activities (38). Mutations in CYC8 (SSN6) and CYC9 (TUP1) both cause overexpression of iso-2-cytochrome c (46), and mutations in GRR1 and CAT4 allow iso-1-cytochrome c to escape glucose repression (17, 48). However, the extent to which these genes may be general regulators of mitochondrial biogenesis remains unknown.Boker-Schmitt and coworkers isolated two constitutively highly respiring mutants, CCR-91 and CCR-96, in which respiration, mitochondrial cytochromes, and mitochondrial DNA synthesis were released from glucose repression (2). Significantly, the activities of maltase and malate synthase, two nonmitochondrial glucose-regulated enzymes, were not changed in the CCR-91 mutant, indicating that glucose repression of mitochondrial biogenesis is at least partially independent of repression of enzymes involved in disaccha...

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Mitochondrial control of cell surface characteristics in Saccharomyces cerevisiae

Cited by 39 publications

References 7 publications

Dual cell wall/mitochondria localization of the âSUNâ family proteins

Dual cell wall/mitochondria localization of the âSUNâ family proteins

Dual cell wall/mitochondria localization of the 'SUN' family proteins

Regulation of nuclear genes encoding mitochondrial proteins in Saccharomyces cerevisiae

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Mitochondrial control of cell surface characteristics in Saccharomyces cerevisiae

Cited by 39 publications

References 7 publications

Dual cell wall/mitochondria localization of the âSUNâ family proteins

Dual cell wall/mitochondria localization of the âSUNâ family proteins

Dual cell wall/mitochondria localization of the 'SUN' family proteins

Regulation of nuclear genes encoding mitochondrial proteins in Saccharomyces cerevisiae

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Dual cell wall/mitochondria localization of the âSUNâ family proteins

Dual cell wall/mitochondria localization of the âSUNâ family proteins