Mitochondrial cox1 and cob sequence diversities in Gelidium vagum (Gelidiales, Rhodophyta) in Korea

Abstract

The number of species of non-native and invasive marine algae is growing, with concomitant public concern about native ecosystems and coastlines. Gelidium vagum, recently introduced from northeast Asia to Europe and North America, commonly occurs from the intertidal to subtidal zones in Korea, China, and Japan. To investigate the level of genetic diversity of native populations, we analyzed mitochondrial cox1 and cob from 108 specimens of G. vagum from Korea, China, eastern Russia, including from the Netherlands and USA. The haplotype network of individual and cox1 + cob datasets revealed no genetic structure in local populations, suggesting genetic flow between Korean populations. Our results corroborate a typical pattern of genetic diversity for introduced species, with low levels in introduced populations and high levels in native populations. All haplotypes were shared between the Netherlands and USA, but not between Korea and the Netherlands / USA except cox1. Additional sampling will identify donor populations in native northeast Asian waters. This is the first report of the utility of the mitochondrial coding cob sequences in red algae.

Cyanidiophyceae in Iceland: plastidrbcL gene elucidates origin and dispersal of extremophilicGaldieria sulphurariaandG. maxima(Galdieriaceae, Rhodophyta)

“…Population expansions were assessed by neutrality tests implemented in DnaSP v.5.10.01 (Librado & Rozas 2009) for Tajima's D (Tajima 1989) and the F* of Fu & Li (1993). Population genetic analyses were conducted using ARLEQUIN v.3.5.1.3 (Excoffier & Lischer 2010), following basic analytic scheme as described in Yoon et al (2014). A hierarchical analysis of molecular variance (AM OVA) framework was used to estimate three molecular variance components, such as among the regions (SWI, SEI, and NEI), among locations within each region, and within each location.…”

Molecular identification of Gracilaria species (Gracilariales, Rhodophyta) obtained from the South Coast of Java Island, Indonesia

“…It has been previously shown that the COX1 gene is applicable in identifying red seaweed species (Yang et al 2013;Yoon et al 2014). This gene is a relatively short DNA portion and is easily amplified and sequenced with one pair of primers.…”