Mitochondrial DNA and genomic DNA ratio in embryo culture medium is not a reliable predictor for in vitro fertilization outcome

Zhang, Xinyue; Sun, Yabing; Dong, Xin; Zhou, Jianxiong; Sun, Fubo; Han, Tingting; Lei, Ping; Mao, Rurong; Guo, Xuzhou; Wang, Qi; Li, Penghao; Qu, Tingyu; Huang, Jihua; Li, Lingxiao; Huang, Tianhua; Zhong, Ying; Gu, Jiang

doi:10.1038/s41598-019-41801-1

Cited by 6 publications

(7 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With the multiple annealing and looping-based amplification cycles method of next-generation sequencing for whole genome amplification, a study reported that the mt/gDNA ratio of medium from embryos that reached blastulation with successful pregnancy showed a decreasing trend, but the differences were not statistically significant. 23 Based on previous research, we reduced the impact of individual differences and the influence of age by selecting standard patients in our center seeking assistance with pregnancy. We also used digital PCR technology, which provided better detection sensitivity and more accurate results.…”

Section: Discussionmentioning

confidence: 99%

“…The small sample size might also account for the results differing between the present and previous studies. 23 We classified blastocysts as high-, -medium, and poor-quality according to the ICM ratings and TE as an integrity factor. The ICM and TE were separately analyzed a previous study.…”

Section: Discussionmentioning

confidence: 99%

“…Embryos can reportedly be screened by detecting mtDNA and genomic (g) DNA based on embryo culture medium; [20][21][22] However others have suggested the opposite. 23 We explored the relationship between the mtDNA/gDNA ratio and embryonic developmental potential by measuring levels of cell-free DNA in embryonic culture medium using digital polymerase chain reaction (dPCR) technology that has better detection accuracy than real-time quantitative (RT-q PCR). Digital PCR is a new method of measuring DNA that independently divides PCR reaction units.…”

Section: Plain Language Summarymentioning

confidence: 99%

“…The ICM and TE were separately analyzed a previous study. 23 We further investigated the relationship between the mtDNA/gDNA ratio and embryo development, by subdividing the unformed blastocysts according to embryo fragmentation. We found a tendency towards increased embryo fragmentation, and a decreased mtDNA/gDNA ratio in the group that did not form blastocysts.…”

Section: Dovepressmentioning

confidence: 99%

See 3 more Smart Citations

Digital PCR Detection of mtDNA/gDNA Ratio in Embryo Culture Medium for Prediction of Embryo Development Potential

Zhang¹,

Ji²,

Shi³

et al. 2021

PGPM

View full text Add to dashboard Cite

Purpose The ratio of mitochondrial DNA to genomic DNA (mtDNA/gDNA) in embryo culture medium as a predictor of embryonic development is a new method of noninvasive embryo screening. However, current tests based on this concept have proven inconsistent. The aim of this study was to define the predictive value of the ratio of mtDNA/gDNA for embryonic developmental potential. Materials and Methods We used digital PCR to measure mtDNA/gDNA ratios in day 3 culture media of 223 embryos from 56 patients. We compared the relationship between the predictive value of mtDNA/gDNA ratio and each of embryo fragmentation, embryo morphological grade, and blastocyst formation. Results mtDNA/gDNA ratio decreased significantly with a decrease in embryo rating: 22.54 (44.66); 31.25 (36.97) and 46.33 (57.11); Grades A vs C, P = 0.006; B vs C, P = 0.015. mtDNA/gDNA ratio increased overall with an increase in embryo fragment content but did not differ significantly between high-, -medium, and poor-quality embryos. Interestingly, this trend differed from that of the unformed blastocysts. mtDNA/gDNA ratio of cleavage stage embryos forming blastocysts was lower ( P =0.005). Trends of mtDNA/gDNA ratio differed according to inner cell mass (ICM) and trophectoderm (TE) levels, but not significantly. mtDNA/gDNA ratio in day 3 culture medium was not significantly improved over morphological scores. Conclusion We hereby show the correlation of mtDNA/gDNA ratio in the culture medium of developing embryos. The correlation between the mtDNA/gDNA ratio and early embryonic development was controversial. Furthermore, an increase in mtDNA/gDNA ratio might indicate reduced development potential, but the difference remains insufficient for application as a clinical predictor.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Plain Language Summarymentioning

confidence: 99%

Section: Dovepressmentioning

confidence: 99%

See 2 more Smart Citations

Digital PCR Detection of mtDNA/gDNA Ratio in Embryo Culture Medium for Prediction of Embryo Development Potential

Zhang¹,

Ji²,

Shi³

et al. 2021

PGPM

View full text Add to dashboard Cite

show abstract

“…However, a low concordance was previously reported between the amount of cfDNA content in the medium and chromosomal abnormalities in embryos obtained from trophectoderm cell (TE) using biopsy analysis [ 18 , 19 ]. The cf-mtDNA/cf-nDNA ratio in the medium was found to not differ between aneuploid and euploid embryos in humans [ 20 ]. However, further evidence is needed to evaluate the amount of cfDNA in the SCM of embryos.…”

mentioning

confidence: 99%

Comparative analysis of cell-free DNA content in culture medium and mitochondrial DNA copy number in porcine parthenogenetically activated embryos

Kobayashi

Ito

Shirasuna

et al. 2020

Journal of Reproduction and Development

View full text Add to dashboard Cite

We examined the effect of ploidy on mitochondrial DNA (mtDNA) copy number in embryos and the amount of cell-free mitochondrial and nucleic DNA content (cf-mtDNA and cf-nDNA) in spent culture medium (SCM). Oocytes collected from the ovaries were matured, activated, incubated in medium containing cycloheximide (CHX) or CHX and cytochalasin B (CB) for 4.5 h to produce haploid or diploid embryos (H-group and D-group embryos). These embryos were cultured for 7 days, and the blastocysts and SCM were examined. The amount of mtDNA and nDNA was determined by real-time PCR. The rate of development to the blastocyst stage was higher for the D-group than for the H-group. Moreover, D-group blastocysts had less mtDNA compared to the H-group blastocysts. After activation, the mitochondrial content was constant before the blastocyst stage in D-group embryos, but increased earlier in H-group embryos. The amount of cf-mtDNA in the SCM of D-group blastocysts was greater than that of H-group blastocysts. However, when the cf-mtDNA in the SCM of 2 cell-stage embryos (day 2 post-activation) was examined, the amount of cf-mtDNA was greater in the H-group than in the D-group embryos. When D-group embryos were cultured for 7 days, a significant correlation was observed between the total cell number of blastocysts and cf-nDNA content in the SCM. Hence, although careful consideration is needed regarding the time point for evaluating mtDNA content in the embryos and SCM, this study demonstrates that mtDNA in the embryos and SCM was affected by the ploidy of the embryos.

show abstract

Biotechnology in the process of assisted reproduction

Toporcerová

Špaková

Mareková

et al. 2022

Biotechnology in Healthcare

View full text Add to dashboard Cite

Mitochondrial DNA and genomic DNA ratio in embryo culture medium is not a reliable predictor for in vitro fertilization outcome

Cited by 6 publications

References 49 publications

Digital PCR Detection of mtDNA/gDNA Ratio in Embryo Culture Medium for Prediction of Embryo Development Potential

Digital PCR Detection of mtDNA/gDNA Ratio in Embryo Culture Medium for Prediction of Embryo Development Potential

Comparative analysis of cell-free DNA content in culture medium and mitochondrial DNA copy number in porcine parthenogenetically activated embryos

Biotechnology in the process of assisted reproduction

Contact Info

Product

Resources

About