Mitochondrial fission protein MoFis1 mediates conidiation and is required for full virulence of the rice blast fungus Magnaporthe oryzae

Khan, Ibrar Muhammad; Ning, Guo-Ao; Liu, Xiaohong; Feng, Xiaoxiao; Lin, Fu‐Cheng; Lu, Jianping

doi:10.1016/j.micres.2015.06.002

Cited by 21 publications

(18 citation statements)

References 51 publications

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“…Fluorescence signal of GFP and RFP was detected and overlapped, indicating that both MoFis1 and MoMdv1 are located to the peroxisomes. Moreover, studies of mitochondria localization of these two proteins revealed that both MoFis1 and MoMdv1 are partially co-localized with mitochondria (Fig 6C–6F), consistent with an earlier report [58]. Taken together, the observation of colocalization supports that MoDnm1, MoFis1, and MoMdv1 function as a complex.…”

Section: Resultssupporting

confidence: 91%

MoDnm1 Dynamin Mediating Peroxisomal and Mitochondrial Fission in Complex with MoFis1 and MoMdv1 Is Important for Development of Functional Appressorium in Magnaporthe oryzae

et al. 2016

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Dynamins are large superfamily GTPase proteins that are involved in various cellular processes including budding of transport vesicles, division of organelles, cytokinesis, and pathogen resistance. Here, we characterized several dynamin-related proteins from the rice blast fungus Magnaporthe oryzae and found that MoDnm1 is required for normal functions, including vegetative growth, conidiogenesis, and full pathogenicity. In addition, we found that MoDnm1 co-localizes with peroxisomes and mitochondria, which is consistent with the conserved role of dynamin proteins. Importantly, MoDnm1-dependent peroxisomal and mitochondrial fission involves functions of mitochondrial fission protein MoFis1 and WD-40 repeat protein MoMdv1. These two proteins display similar cellular functions and subcellular localizations as MoDnm1, and are also required for full pathogenicity. Further studies showed that MoDnm1, MoFis1 and MoMdv1 are in complex to regulate not only peroxisomal and mitochondrial fission, pexophagy and mitophagy progression, but also appressorium function and host penetration. In summary, our studies provide new insights into how MoDnm1 interacts with its partner proteins to mediate peroxisomal and mitochondrial functions and how such regulatory events may link to differentiation and pathogenicity in the rice blast fungus.

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Section: Resultssupporting

confidence: 91%

MoDnm1 Dynamin Mediating Peroxisomal and Mitochondrial Fission in Complex with MoFis1 and MoMdv1 Is Important for Development of Functional Appressorium in Magnaporthe oryzae

et al. 2016

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“…The adaptation of mitochondrial fusion and fission to cellular demands is critical for a number of important physiological processes (Zemirli and Morel, ). In M. oryzae , the complex composed of MoDnm1, MoFis1 and MoMdv1 regulates mitochondrial fission and plays important roles in pathogenesis (Khan et al , ; Zhong et al , ). In this study, the regulation and function of mitochondrial dynamics during M. oryzae growth and development in planta was determined.…”

Section: Discussionmentioning

confidence: 99%

“…Recent studies have shown that the complex composed of MoDnm1, MoFis1 and MoMdv1 regulates the mitochondrial fission in M. oryzae (Zhong et al , ). Disruption of MoDNM1 or MoFIS1 results in defects in mitochondria fission and pathogenicity (Khan et al , ; Zhong et al , ). However, the regulation and function of mitochondrial dynamics during in planta development in M. oryzae remains to be investigated further.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial dynamics and mitophagy are necessary for proper invasive growth in rice blast

Kou

Qiu

et al. 2019

Molecular Plant Pathology

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Summary Magnaporthe oryzae causes blast disease, which is one of the most devastating infections in rice and several important cereal crops. Magnaporthe oryzae needs to coordinate gene regulation, morphological changes, nutrient acquisition and host evasion in order to invade and proliferate within the plant tissues. Thus far, the molecular mechanisms underlying the regulation of invasive growth in planta have remained largely unknown. We identified a precise filamentous‐punctate‐filamentous cycle in mitochondrial morphology during Magnaporthe –rice interaction. Interestingly, disruption of such mitochondrial dynamics by deletion of genes regulating either the mitochondrial fusion (MoFzo1) or fission (MoDnm1) machinery, or inhibition of mitochondrial fission using Mdivi‐1 caused significant reduction in M. oryzae pathogenicity. Furthermore, exogenous carbon source(s) but not antioxidant treatment delayed such mitochondrial dynamics/transition during invasive growth. In contrast, carbon starvation induced the breakdown of the mitochondrial network and led to more punctate mitochondria in vitro . Such nutrient‐based regulation of organellar dynamics preceded MoAtg24‐mediated mitophagy, which was found to be essential for proper biotrophic development and invasive growth in planta . We propose that precise mitochondrial dynamics and mitophagy occur during the transition from biotrophy to necrotrophy and are required for proper induction and establishment of the blast disease in rice.

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“…S4, see Supporting Information). Importantly, the GFP‐PEF1 protein was strongly co‐localized with M. oryzae MoFis1 (Khan et al ., ), the orthologue of S. cerevisiae Fis1 (Fig. S5, see Supporting Information).…”

Section: Resultsmentioning

confidence: 99%

Peroxisomal fission is induced during appressorium formation and is required for full virulence of the rice blast fungus

Chen

Shen

Yang

et al. 2016

Molecular Plant Pathology

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Peroxisomes are involved in various metabolic processes and are important for virulence in different pathogenic fungi. How peroxisomes rapidly emerge in the appressorium during fungal infection is poorly understood. Here, we describe a gene, PEF1, which can regulate peroxisome formation in the appressorium by controlling peroxisomal fission, and is required for plant infection in the rice blast fungus Magnaporthe oryzae. Targeted deletion of PEF1 resulted in a reduction in virulence and a delay in penetration and invasive growth in host cells. PEF1 was particularly expressed during appressorial development, and its encoding protein was co-localized with peroxisomes during appressorial development. Compared with the massive vesicle-shaped peroxisomes formed in the wild-type appressorium, the Δpef1 mutant could only form stringy linked immature peroxisomes, suggesting that PEF1 was involved in peroxisomal fission during appressorium formation. We also found that the Δpef1 mutant could not utilize fatty acids efficiently, which can improve significantly the expression level of PEF1 and induce peroxisomal fission. As expected, the Δpef1 mutant showed reduced intracellular production of reactive oxygen species (ROS) during appressorium formation and induced ROS accumulation in host cells during infection. Taken together, PEF1-mediated peroxisomal fission is important for fungal infection by controlling the number of peroxisomes in the appressorium.

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Mitochondrial fission protein MoFis1 mediates conidiation and is required for full virulence of the rice blast fungus Magnaporthe oryzae

Cited by 21 publications

References 51 publications

MoDnm1 Dynamin Mediating Peroxisomal and Mitochondrial Fission in Complex with MoFis1 and MoMdv1 Is Important for Development of Functional Appressorium in Magnaporthe oryzae

MoDnm1 Dynamin Mediating Peroxisomal and Mitochondrial Fission in Complex with MoFis1 and MoMdv1 Is Important for Development of Functional Appressorium in Magnaporthe oryzae

Mitochondrial dynamics and mitophagy are necessary for proper invasive growth in rice blast

Peroxisomal fission is induced during appressorium formation and is required for full virulence of the rice blast fungus

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