Mitochondrial function, blastocyst development and live foals born after ICSI of immature vitrified/warmed equine oocytes matured with or without melatonin

Clérico, G.; Taminelli, G.; Veronesi, J.C.; Polola, J.; Pagura, N.; Pinto, Carlos; Sansinena, M.

doi:10.1016/j.theriogenology.2020.10.036

Cited by 27 publications

(28 citation statements)

References 54 publications

(68 reference statements)

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“…The cryopreservation of mouse [ 27 ], bovine [ 50 ], pig [ 51 ], and human oocytes [ 52 ] increases oxidative stress, although exogenous melatonin reduce oxidative stress in vitrified-warmed oocytes [ 30 , 53 ]. The Nrf2 signaling pathway is an important intracellular antioxidant signaling pathway, critical for maintaining redox balance [ 31 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

Melatonin Promotes In Vitro Maturation of Vitrified-Warmed Mouse Germinal Vesicle Oocytes, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway

Guo

Yang

Qin

et al. 2021

Animals

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Previously it was reported that melatonin could mitigate oxidative stress caused by oocyte cryopreservation; however, the underlying molecular mechanisms which cause this remain unclear. The objective was to explore whether melatonin could reduce oxidative stress during in vitro maturation of vitrified-warmed mouse germinal vesicle (GV) oocytes through the Nrf2 signaling pathway or its receptors. During in vitro maturation of vitrified-warmed mouse GV oocytes, there were decreases (p < 0.05) in the development rates of metaphase I (MI) oocytes and metaphase II (MII) and spindle morphology grades; increases (p < 0.05) in the reactive oxygen species (ROS) levels; and decreases (p < 0.05) in expressions of Nrf2 signaling pathway-related genes (Nrf2, SOD1) and proteins (Nrf2, HO-1). However, adding 10−7 mol/L melatonin to both the warming solution and maturation solutions improved (p < 0.05) these indicators. When the Nrf2 protein was specifically inhibited by Brusatol, melatonin did not increase development rates, spindle morphology grades, genes, or protein expressions, nor did it reduce vitrification-induced intracellular oxidative stress in GV oocytes during in vitro maturation. In addition, when melatonin receptors were inhibited by luzindole, the ability of melatonin to scavenge intracellular ROS was decreased, and the expressions of genes (Nrf2, SOD1) and proteins (Nrf2, HO-1) were not restored to control levels. Therefore, we concluded that 10−7 mol/L melatonin acted on the Nrf2 signaling pathway through its receptors to regulate the expression of genes (Nrf2, SOD1) and proteins (Nrf2, HO-1), and mitigate intracellular oxidative stress, thereby enhancing in vitro development of vitrified-warmed mouse GV oocytes.

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Section: Discussionmentioning

confidence: 99%

Melatonin Promotes In Vitro Maturation of Vitrified-Warmed Mouse Germinal Vesicle Oocytes, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway

Guo

Yang

Qin

et al. 2021

Animals

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“…In the early phase of technological development, maturation rates of vitrified immature oocytes were poor (less than 20%) [87,89]. Recently, results have been much improved and range from 33.3% to 54% [61,[90][91][92][93]. Following fertilization via ICSI, the vitrified-warmed oocytes could develop into cleavage-and blastocyst-stage embryos, but the efficiency is still poor when compared to nonfrozen oocytes [92,94].…”

Section: Horsementioning

confidence: 99%

“…First reports of pregnancy were obtained by the transfer of vitrified matured oocytes into the oviducts of inseminated mares [60]. More recently, pregnancy from vitrified oocytes and in vitro embryo culture could be obtained, with pregnancy rates of 17-50% and resulting in live foals [61,93].…”

Section: Horsementioning

confidence: 99%

Oocyte Cryopreservation in Domestic Animals and Humans: Principles, Techniques and Updated Outcomes

Tharasanit

Thuwanut

2021

Animals

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Oocyte cryopreservation plays important roles in basic research and the application of models for genetic preservation and in clinical situations. This technology provides long-term storage of gametes for genetic banking and subsequent use with other assisted reproductive technologies. Until recently, oocytes have remained the most difficult cell type to freeze, as the oocytes per se are large with limited surface area to cytoplasm ratio. They are also highly sensitive to damage during cryopreservation, and therefore the success rate of oocyte cryopreservation is generally poor when compared to noncryopreserved oocytes. Although advancement in oocyte cryopreservation has progressed rapidly for decades, the improvement of cryosurvival and clinical outcomes is still required. This review focuses on the principles, techniques, outcomes and prospects of oocyte cryopreservation in domestic animals and humans.

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“… 27 , 28 Therefore, the effect of antioxidants on oocytes and embryo following vitrification has been extensively studied; supplementation of culture medium of vitrified‐warmed oocytes or embryos with melatonin, glutathione ethyl‐ester, N‐acetyl cysteine, α‐tocopherol, or coenzyme Q reduced ROS levels and improved oocyte viability as well as embryo development in equine, cows, and mice. 6 , 29 , 30 , 31 , 32 In addition, supplementation of vitrified‐warming solution with acetyl‐L‐carnitine, N‐acetyl‐cysteine, and lipoic acid improved the cell number of the blastocysts and fetal development in mice. 33 Furthermore, addition of vitamin E to the culture medium of vitrified‐warming ovarian tissue improved oocyte and subsequent blastocyst stage development.…”

Section: Antioxidants Protect Vitrified‐warmed Oocytes and Embryosmentioning

confidence: 99%

Resveratrol enhanced mitochondrial recovery from cryopreservation‐induced damages in oocytes and embryos

Iwata

2021

Reprod Medicine & Biology

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Embryo transfer (ET) is a major assisted reproductive technology (ART) used for domestic animals and humans worldwide. In industrialized countries, an increasing number of women get pregnant using ET technology. Success in ET depends on the quality of embryos, which are affected by physiological conditions of oocyte donors such as obesity and diabetes, maternal age, and an ART per se, including in vitro culture and cryopreservation. Cryopreservation is pivotal in ART and supports the widespread use of the embryos collected from genetically and/or commercially valuable domestic animals, as it increases opportunity for pregnancy. Cryopreservation methods have improved over decades; oocytes that are usually difficult to cryopreserve now have high survival rate (over 80%) after

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Mitochondrial function, blastocyst development and live foals born after ICSI of immature vitrified/warmed equine oocytes matured with or without melatonin

Cited by 27 publications

References 54 publications

Melatonin Promotes In Vitro Maturation of Vitrified-Warmed Mouse Germinal Vesicle Oocytes, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway

Melatonin Promotes In Vitro Maturation of Vitrified-Warmed Mouse Germinal Vesicle Oocytes, Potentially by Reducing Oxidative Stress through the Nrf2 Pathway

Oocyte Cryopreservation in Domestic Animals and Humans: Principles, Techniques and Updated Outcomes

Resveratrol enhanced mitochondrial recovery from cryopreservation‐induced damages in oocytes and embryos

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