Zaprionus indianus Gupta (Diptera: Drosophilidae) is a fly species native to the Afrotropical biogeographic region (Chassagnar & Kraaijeveld, 1991; Yassin et al., 2008a,b). About 40 years ago, Z. indianus extended its geographical distribution from its native range in Africa to other areas in the world (Commar et al., 2012). It was found for the first time in South America in 1999 in the São Paulo city area, near the Atlantic coast of Brazil (Vilela, 1999). Since this initial record, Z. indianus has spread throughout the American continent, reaching latitudes from 34°S to 45-50°N (Lavagnino et al., 2020a). The most recent report is form Colombia (Butsca, 2021). Zaprionus indianus is now an established non-native species in the American continent that maintains stable populations in many natural or semi-natural environments and often expands its distribution (Commar et al., 2012;Fernandez Goya et al., 2020; Lavagnino et al., 2020a,b,c). Similar to other drosophilid species (Markow & O'Grady, 2005), females of this species lay eggs on rotten or damaged fruits that have been colonized by yeasts and bacteria, behaving as a secondary pest (