Mitochondrial genome of the critically endangered smalltooth sawfish Pristis pectinata from Veracruz, Mexico

Díaz‐Jaimes, Píndaro; Bonfil, Ramón; Palacios-Barreto, Paola; Bolaño-Martínez, Nataly; Bayona‐Vásquez, Natalia J.

doi:10.1007/s12686-017-0896-9

Cited by 7 publications

(3 citation statements)

References 8 publications

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“…Catoche and Isla Contoy in 2020. These recent reports align with the catch of a small-toothed sawfish (P. pectinata) in Barra de Cazones, Veracruz, in 2015 (Díaz et al, 2017), as well as environmental DNA samples that tested positive for Pristis spp. In Veracruz and Campeche in 2016 and 2017, respectively (Bonfil et al, 2021).…”

Section: Lek Of Sawfishes To Inspire Future Researchsupporting

confidence: 70%

Interdisciplinary science and fishers’ local ecological knowledge of sawfishes in the Yucatán Peninsula

Rubio‐Cisneros,

Martínez‐Candelas,

Ordaz‐García

et al. 2023

Aquatic Conservation

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Our knowledge of sawfishes remains scant for Latin America. Pristis pristis (largetooth sawfish) and Pristis pectinata (smalltooth sawfish) are critically endangered. In the Yucatán Peninsula (YP), P. pristis and P. pectinata inhabit coastal landscapes. A total of 290 surveys of fishers’ local ecological knowledge (LEK), including a geospatial component, were collected; 74 literature sources and available archaeological data for the region were reviewed. Interdisciplinary results show the following: the common past presence of sawfishes, their cultural significance for coastal societies, and the contemporary absence of sawfishes in coastal areas where they existed historically; sightings of juveniles were only documented by elder fishers, and only two recent narratives mention sawfishes caught incidentally 5 years ago. Geospatial results provide evidence for 52 geographic sites where sawfishes were common in the YP. Results support the development of research methodologies to study human–nature interactions integrating LEK. This matters in the YP, where sociocultural values and landscapes have changed rapidly through increasing tourism development and human overcrowding. The results can help conservation managers understand the past presence and contemporary loss of sawfishes, and their habitat, and contribute to understanding the defaunation of megafauna in the YP through time. This is critical for tourism and fishery ecosystem services on which communities of the YP rely for their economies.

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Section: Lek Of Sawfishes To Inspire Future Researchsupporting

confidence: 70%

Interdisciplinary science and fishers’ local ecological knowledge of sawfishes in the Yucatán Peninsula

Rubio‐Cisneros,

Martínez‐Candelas,

Ordaz‐García

et al. 2023

Aquatic Conservation

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“…It is promising to note that there are no bp differences in the primer or probe sequences designed in this study compared to a mtDNA ND2 sequence for a P. pectinata collected in Mexico (GenBank accession no. MF682494.1; Diaz-Jaimes et al 2018), indicating the developed assay should amplify the target gene in this species from other, nearby waters in the Western Atlantic. Use of the assay outside these waters requires careful consideration and, potentially, further testing.…”

Section: Discussionmentioning

confidence: 99%

An environmental DNA tool for monitoring the status of the Critically Endangered Smalltooth Sawfish, Pristis pectinata, in the Western Atlantic

Lehman

Poulakis

Scharer

et al. 2019

Preprint

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AbstractThe Critically Endangered Smalltooth Sawfish, Pristis pectinata, was once widespread in the tropical and subtropical waters of the Atlantic Ocean, but following substantial declines over the past century, the core population is currently confined to southwest Florida in the U.S. and the Bahamas. Recent research and verified public encounter reports suggests that this core population may be stabilizing and, potentially, expanding into formerly occupied areas of their historic range in the Western Atlantic; however, the status of this species in non-core waters is not well understood. Environmental DNA (eDNA) methods provide a relatively cost effective and rapid assessment tool for monitoring species occurrence in aquatic habitats. Here, we have developed an eDNA tool: a species-specific Droplet Digital™ PCR (ddPCR™) assay targeting a 100-base pair portion of the mitochondrial NADH dehydrogenase subunit 2 gene in P. pectinata, with the ability to reliably detect as little as 0.25 pg of target DNA. The assay was validated by collecting and analyzing a water sample from known P. pectinata nursery habitat in Florida, which was found to contain an average of 11.54 copies of target DNA/µL (SE = 0.72) in the reaction. The assay was then further tested by placing a juvenile sawfish in an ex situ tank and analyzing water samples collected at time intervals. The implementation of this eDNA tool into field surveys will provide additional, reliable data to assess species recovery and aid in prioritizing localities beyond the core range in which to focus research and education initiatives.

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“…Extraction blanks were included to test for contamination during this process. For samples from the P. pectinata aquarium specimen and from the tissue collected from the dried rostrum of P. pristis, we used a traditional phenol:chloroform:isoamyl alcohol (25:24:1 v/v) protocol (Dıáz-Jaimes et al, 2018).…”

Section: Dna Extractionmentioning

confidence: 99%

Improved eDNA assay evidences further refugia for critically endangered smalltooth sawfish (Pristis pectinata) in Mexico

Bonfil,

Díaz-Jaimes,

Palacios-Barreto

et al. 2024

Front. Mar. Sci.

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Sawfishes are considered one of the most threatened marine fish taxa globally, with major contractions in their geographic range documented over the last 50 years across all oceans. Two sawfish species used to be found in Mexico, but a historical lack of research and first-hand baseline information recently raised concerns about the fate of both species there. Recent Local Ecological Knowledge (LEK) and environmental DNA (eDNA) research has shown that: a) Pristis pectinata and P. pristis were formerly abundant and widely distributed in the Mexican Atlantic coast, and the latter also in the Mexican Pacific coast, and b) sawfishes (at least P. pectinata) are still found in some coastal refugia in central Veracruz state and Campeche state on the Atlantic coast. In the present research we developed improved primers that allow for better species ID resolution for Pristis pectinata and P. pristis through successful sequencing of eDNA samples by amplifying a 270 bp fragment of the CO1 gene. Our results based on the analysis of 305 eDNA samples showed an improved positive species identification rate through sequencing, demonstrate that P. pectinata occurs in other coastal refugia along the Yucatán Peninsula in the Mexican Caribbean, and suggest this species might be extirpated from southern Veracruz, while no P. pristis eDNA has been found along the Atlantic coast.

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Mitochondrial genome of the critically endangered smalltooth sawfish Pristis pectinata from Veracruz, Mexico

Cited by 7 publications

References 8 publications

Interdisciplinary science and fishers’ local ecological knowledge of sawfishes in the Yucatán Peninsula

Interdisciplinary science and fishers’ local ecological knowledge of sawfishes in the Yucatán Peninsula

An environmental DNA tool for monitoring the status of the Critically Endangered Smalltooth Sawfish, Pristis pectinata, in the Western Atlantic

Improved eDNA assay evidences further refugia for critically endangered smalltooth sawfish (Pristis pectinata) in Mexico

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