Mitochondrial genomes of the freshwater monogonont rotifer <i>Brachionus fernandoi</i> and of two additional <i>B. calyciflorus</i> sensu stricto lineages from Germany and the USA (Rotifera, Brachionidae)

Kiemel, Katrin; Cahsan, Binia De; Paraskevopoulou, Sofia; Weithoff, Guntram; Tiedemann, Ralph

doi:10.1080/23802359.2022.2060765

Cited by 3 publications

(2 citation statements)

References 18 publications

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“…To estimate the within B. calyciflorus species complex divergence time between B. calyciflorus s.s. and B. fernandoi the program BEAST v.1.10.4 120 was used. Whole mtGenome coding sequences (cds) of different rotifer species were downloaded from NCBI: B. calyciflorus s.s. 121 , B. fernandoi 121 , B. angularis 122 , B. manjavacas 123 , B. plicatilis 124 , B. koreanus 125 , B. rotundiformis 126 , B. rubens 127 and P. similis 128 (for Genebank accession numbers cf. Supplementary figure s8 ).…”

Section: Methodsmentioning

confidence: 99%

Variation in heat shock protein 40 kDa relates to divergence in thermotolerance among cryptic rotifer species

Kiemel

Gurke

Paraskevopoulou

et al. 2022

Sci Rep

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Genetic divergence and the frequency of hybridization are central for defining species delimitations, especially among cryptic species where morphological differences are merely absent. Rotifers are known for their high cryptic diversity and therefore are ideal model organisms to investigate such patterns. Here, we used the recently resolved Brachionus calyciflorus species complex to investigate whether previously observed between species differences in thermotolerance and gene expression are also reflected in their genomic footprint. We identified a Heat Shock Protein gene (HSP 40 kDa) which exhibits cross species pronounced sequence variation. This gene exhibits species-specific fixed sites, alleles, and sites putatively under positive selection. These sites are located in protein binding regions involved in chaperoning and may therefore reflect adaptive diversification. By comparing three genetic markers (ITS, COI, HSP 40 kDa), we revealed hybridization events between the cryptic species. The low frequency of introgressive haplotypes/alleles suggest a tight, but not fully impermeable boundary between the cryptic species.

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Section: Methodsmentioning

confidence: 99%

Variation in heat shock protein 40 kDa relates to divergence in thermotolerance among cryptic rotifer species

Kiemel

Gurke

Paraskevopoulou

et al. 2022

Sci Rep

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“…We focused on the genus Brachionus , as they are abundant in rift valley lakes (e.g., Burian et al, 2013; Vareschi & Vareschi, 1984). Furthermore, their asexual reproduction leads to the accumulation of genetically identical individuals (clones) which are distinguishable by their mitochondrial DNA (e.g., Kiemel et al, 2022a) and clone successions previously have been associated with environmental perturbation (Epp et al, 2010). We aimed to unravel whether rotifer seda DNA reflect past climate changes and record the onset of increasing anthropogenic impact.…”

Section: Introductionmentioning

confidence: 99%

Sedimentary ancient DNA of rotifers reveals responses to 200 years of climate change in two Kenyan crater lakes

et al. 2023

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Sedimentary ancient DNA (sedaDNA) has proven to be a useful tool for palaeoenvironmental studies, but only a handful of studies exist so far for tropical regions. In this study we used sedaDNA to study the temporal succession of Brachionus spp. rotifer mitochondrial DNA haplotypes using two sediment cores from two climatically different alkaline‐saline crater lakes from the Kenyan Rift Valley. Data were retrieved from a sediment core (dating back to AD 1800) from Lake Kageinya, located in the remote, hot and hyper‐arid Suguta Valley. sedaDNA was used to study the temporal succession of mitochondrial DNA haplotypes of Brachionus spp. rotifers. The results were compared to previously published data from Lake Sonachi, a well‐studied lake in the humid and colder mountainous region of Kenya near the town of Naivasha, now supported by a 210Pb age chronology. Both records extend back before the onset of substantial anthropogenic impact in these regions. The results revealed that climate—rather than anthropogenic impact—was most strongly correlated with haplotype changes in both lakes. During prolonged dry periods (such as from AD 1910 to the late AD 1960s), certain haplotypes persisted. Sudden changes and the emergence of alter native haplotypes were observed when climate became more humid or during episodes of highly variable climate (before AD 1910 and from AD 1960s onwards). Progressive changes in prevailing haplotypes during periods with variable climate could reflect local adaptation and/or be the result of immigration of new haplotypes after the eradication of previous populations during extreme environmental conditions (high temperatures, UV irradiation, pH and salinity). The results indicate that, despite adverse chemical conditions, sedaDNA in tropical lake sediments is preserved for at least a few hundred years. Therefore, its analysis provides a useful complementary palaeoenvironmental proxy for palaeolimnological reconstructions and novel insights on changes in rotifer populations through time.

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Variation in Heat Shock Protein 40kDa relates to divergence in thermotolerance among cryptic rotifer species

Kiemel

Gurke

Paraskevopoulou

et al. 2022

Preprint

View full text Add to dashboard Cite

Genetic divergence and the frequency of hybridization are central for defining species delimitations, especially among cryptic species where morphological differences are merely absent. Rotifers are known for their high cryptic diversity and therefore are ideal model organisms to investigate such patterns. Here, we used the recently resolved Brachionus calyciflorus species complex to investigate whether previously observed between species differences in thermotolerance and gene expression are also reflected in their genomic footprint. We identified a Heat Shock Protein gene (HSP 40kDa) which exhibits cross species pronounced structural variation. This gene exhibits species-specific fixed sites, alleles, and sites putatively under positive selection. These sites are located in protein binding regions involved in chaperoning and may therefore reflect adaptive diversification. By comparing three genetic markers (ITS, COI, HSP 40kDa), we revealed hybridization events between the cryptic species. The low frequency of introgressive haplotypes/alleles suggest a tight, but not fully impermeable boundary between the cryptic species.

show abstract

Mitochondrial genomes of the freshwater monogonont rotifer Brachionus fernandoi and of two additional B. calyciflorus sensu stricto lineages from Germany and the USA (Rotifera, Brachionidae)

Cited by 3 publications

References 18 publications

Variation in heat shock protein 40 kDa relates to divergence in thermotolerance among cryptic rotifer species

Variation in heat shock protein 40 kDa relates to divergence in thermotolerance among cryptic rotifer species

Sedimentary ancient DNA of rotifers reveals responses to 200 years of climate change in two Kenyan crater lakes

Variation in Heat Shock Protein 40kDa relates to divergence in thermotolerance among cryptic rotifer species

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