Mitochondrial genomes of the green macroalga Ulva pertusa (Ulvophyceae, Chlorophyta): novel insights into the evolution of mitogenomes in the Ulvophyceae

Abstract

To further understand the trends in the evolution of mitochondrial genomes (mitogenomes or mtDNAs) in the Ulvophyceae, the mitogenomes of two separate thalli of Ulva pertusa were sequenced. Two U. pertusa mitogenomes (Up1 and Up2) were 69,333 bp and 64,602 bp in length. These mitogenomes shared two ribosomal RNAs (rRNAs), 28 transfer RNAs (tRNAs), 29 protein-coding genes, and 12 open reading frames. The 4.7 kb difference in size was attributed to variation in intron content and tandem repeat regions. A total of six introns were present in the smaller U. pertusa mtDNA (Up2), while the larger mtDNA (Up1) had eight. The larger mtDNA had two additional group II introns in two genes (cox1 and cox2) and tandem duplication mutations in noncoding regions. Our results showed the first case of intraspecific variation in chlorophytan mitogenomes and provided further genomic data for the undersampled Ulvophyceae.

Mitochondrial and chloroplast genome sequences of Ulva ohnoi, a green-tide-forming macroalga in the Southern coastal regions of Japan

“…All the protein-coding genes were conserved among Ulva sp. UNA00071828 (Melton et al 2015), U. pertusa (Liu et al 2017), U. fasciata (Melton & Lopez-Bautista 2016), U. prolifera (Liu and Jun Pang 2015), U. linza (Zhou et al 2016) and U. flexuosa (Cai, Wang, Jiang et al 2017). U. ohnoi possessed trnS (GCA), but the gene was lacking in the other Ulva species.…”

Mitogenome analysis of a green tide forming Ulva from California, USA confirms its identity as Ulva expansa (Ulvaceae, Chlorophyta)

“…The two Ulva genomes contain cob, 2 rRNAs, 3 cox, 3 rpl, 5 ATP synthases, 7 orfs, 8 nad, 9 rps, and 27 tRNAs. Gene content, organization, and length of the blooming Ulva was similar to U. pertusa (Liu et al 2017). Phylogenetic analysis of the two mitogenomes indicates a sister relationship to U. pertusa (Figure 1).…”

Ultrasensitive electrocatalytic detection of COX-2 rs20417: relying on 3D interconnected architecture of Pt-LSSUs@PAA nanostructures for sensor interface modification

“…Various methods have been developed for the detection of the COX-2 mutation at the DNA level. Among them is the polymerase chain reaction (PCR) or DNA sequencing [5][6][7]. DNA sequencing is an excellent approach for research studies that seek to profile large regions of DNA, but its implementation is prohibitively expensive for routine clinical use and the slow turnaround time (2-3 weeks) is not an ideal for optimal treatment outcomes.…”