Mitochondrial genomes of three Tetrigoidea species and phylogeny of Tetrigoidea

Abstract

The mitochondrial genomes (mitogenomes) of Formosatettix qinlingensis, Coptotettix longjiangensis and Thoradonta obtusilobata (Orthoptera: Caelifera: Tetrigoidea) were sequenced in this study, and almost the entire mitogenomes of these species were determined. The mitogenome sequences obtained for the three species were 15,180, 14,495 and 14,538 bp in length, respectively, and each sequence included 13 protein-coding genes (PCGs), partial sequences of rRNA genes (rRNAs), tRNA genes (tRNAs) and a A + T-rich region. The order and orientation of the gene arrangement pattern were identical to that of most Tetrigoidea species. Some conserved spacer sequences between trnS(UCN) and nad1 were useful to identify Tetrigoidea and Acridoidea. The Ka/Ks value of atp8 between Trachytettix bufo and other four Tetrigoidea species indicated that some varied sites in this gene might be related with the evolution of T. bufo. The three Tetrigoidea species were compared with other Caelifera. At the superfamily level, conserved sequences were observed in intergenic spacers, which can be used for superfamily level identification between Tetrigoidea and Acridoidea. Furthermore, a phylogenomic analysis was conducted based on the concatenated data sets from mitogenome sequences of 24 species of Orthoptera in the superorders Caelifera and Ensifera. Both maximum likelihood and bayesian inference analyses strongly supported Acridoidea and Tetrigoidea as forming monophyletic groups. The relationships among six Tetrigoidea species were (((((Tetrix japonica, Alulatettix yunnanensis), Formosatettix qinlingensis), Coptotettix longjiangensis), Trachytettix bufo), Thoradonta obtusilobata).

Transcriptome of the pygmy grasshopper Formosatettix qinlingensis (Orthoptera: Tetrigidae)

“…So far, due to small size and limited distribution, the physiological, ecological and genomic studies of the F. qinlingensis were limited, which requires more in-depth research. Among them, Lin et al (2017) assembled and analyzed the mitochondrial genome of F. qinlingensis .…”

Mitochondrial genomes of eight Scelimeninae species (Orthoptera) and their phylogenetic implications within Tetrigoidea

“…Complete mitogenomes have been shown to provide more highly supported results than single or multiple mitochondrial genes ( Yuan et al, 2016 ; Liu et al, 2019 ). Until recently, only 18 mitogenome sequences of Tetrigidae have been submitted to the GenBank ( ; last visited on July 30, 2020) ( Xiao et al, 2012 ; Lin et al, 2017 ; Sun et al, 2017 ; Yang et al, 2017 ; Chang et al, 2020 ). Therefore, more mitogenomes of Tetrigidae are required for a comprehensive phylogenetic analysis, and before a full picture of the mitogenome for Tetrigidae can be depicted.…”

Comparative Mitogenomics and Phylogenetic Implications for Nine Species of the Subfamily Meconematinae (Orthoptera: Tettigoniidae)

“…The base content of the mitochondrial genomes from the nine species showed a significant A + T bias, with the content ranging from 68.99% to 73.61%. The lowest content was found in Phlugiolopsis tri- Long intergenic noncoding spacers (100-500 bp) have also been found in Lepidoptera [49], Coleoptera [50], and other orthopterans [15,19]. The number of overlapping regions in the nine species was greater than the number of interval regions.…”