Mitochondrial mediation of environmental osmolytes discrimination during osmoadaptation in the extremely halotolerant black yeast Hortaea werneckii

Vaupotič, Tomaž; Veranič, Peter; Jenoe, Paul; Plemenitaš, Ana

doi:10.1016/j.fgb.2008.01.006

Cited by 29 publications

(15 citation statements)

References 54 publications

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“…The relationship between stress adaptation and energy metabolism has been shown by others. During treatment with peroxide or high salinity, genes associated with energy production and mitochondrial functions are upregulated in S. cerevisiae (48,59) and the halotolerant black yeast, Hortaea werneckii (53). In S. cerevisiae, respiration-associated transcripts (metabolism and energy functions) were especially strongly induced within 30 min of osmotic stress and persisted throughout exposure (59).…”

Section: Discussionmentioning

confidence: 99%

“…Clearly, osmotic stress can cause the loss of mitochondrial functions and apoptotic programmed cell death, as evidenced by mitochondrial swelling, reduction of mitochondrial inner membranes, and increased production of ROS (51). Growth of H. werneckii in a hypersaline medium resulted in increased ATP synthesis and oxidative damage protection (53). In the same study, the authors identified the 14-3-3 protein-encoding gene HwBMH1 that localized to the mitochondria during hypersaline stress but not during growth of cells in physiological saline.…”

Section: Discussionmentioning

confidence: 99%

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Goa1p of Candida albicans Localizes to the Mitochondria during Stress and Is Required for Mitochondrial Function and Virulence

et al. 2009

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Using a Tn7 transposon library of Candida albicans, we have identified a mutant that exhibited sensitivity in drop plate assays to oxidants such as menadione and hydrogen peroxide. To verify the role of the mutated gene in stress adaptation, null mutants were constructed and phenotypically characterized. Because of its apparent functions in growth and oxidant adaptation, we have named the gene GOA1. Goa1p appears to be unique to the CTG subclade of the Saccharomycotina, including C. albicans. Mutants of C. albicans lacking goa1 (strain GOA31) were more sensitive to 6 mM H 2 O 2 and 0.125 mM menadione than the wild type (wt) or a genereconstituted (GOA32) strain. The sensitivity to oxidants correlated with reduced survival of the GOA31 mutant in human neutrophils and avirulence compared to control strains. Other phenotypes of GOA31 include reduced growth and filamentation in 10% serum, Spider, and SLAD agar media and an inability to form chlamydospores. Since Goa1p has an N-terminal mitochondrion localization site, we also show that green fluorescent protein-tagged Goa1p shows a mitochondrionlike distribution during oxidant or osmotic stress. Further, the inability of GOA31 to grow in medium containing lactate, ethanol, or glycerol as the sole carbon source indicates that the mitochondria are defective in the mutant. To determine how Goa1p contributes to mitochondrial function, we compared the wt, GOA32, and GOA31 strains for mitochondrial electrical membrane potential, respiration, and oxidative phosphorylation. We now show that GOA31, but not the wt or GOA32, had decreased respiration and mitochondrial membrane potential such that mutant cells are unable to drive oxidative phosphorylation. This is the first report in C. albicans of a respiratory defect caused by a loss of mitochondrial membrane potential.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Goa1p of Candida albicans Localizes to the Mitochondria during Stress and Is Required for Mitochondrial Function and Virulence

et al. 2009

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“…Capillary liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) was conducted with an Orbitrap FT hybrid instrument (Thermo Finnigan, San Jose, CA) (17). The MS/MS spectra were searched against the NCBI data bank (using the Matrix Science portal) by applying the following constraints: carbamidomethyl cysteine as fixed and N-acetylation and oxidized methionine as variable modifications; tryptic specificity allowing two missed cleavages; peptide tolerance, 10 ppm; MS/MS tolerance, 0.6 Da; taxonomy setting, bacteria.…”

mentioning

confidence: 99%

Shedding Light on Selenium Biomineralization: Proteins Associated with Bionanominerals

Lenz

Kolvenbach

Gygax

et al. 2011

Appl Environ Microbiol

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Selenium-reducing microorganisms produce elemental selenium nanoparticles with particular physicochemical properties due to an associated organic fraction. This study identified high-affinity proteins associated with such bionanominerals and with nonbiogenic elemental selenium. Proteins with an anticipated functional role in selenium reduction, such as a metalloid reductase, were found to be associated with nanoparticles formed by one selenium respirer, Sulfurospirillum barnesii.

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“…Fungi are a large group of eukaryotic organisms with worldwide distribution, inhabiting a diverse range of extreme habitats from deserts to hypersaline environments [43]. Investigations of the distribution of fungi in extremely hypersaline environments are rare.…”

Section: Microfungimentioning

confidence: 99%

Biodiversity of the Hypersaline Urmia Lake National Park (NW Iran)

et al. 2014

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Urmia Lake, with a surface area between 4000 to 6000 km2, is a hypersaline lake located in northwest Iran. It is the saltiest large lake in the world that supports life. Urmia Lake National Park is the home of an almost endemic crustacean species known as the brine shrimp, Artemia urmiana. Other forms of life include several species of algae, bacteria, microfungi, plants, birds, reptiles, amphibians and mammals. As a consequence of this unique biodiversity, this lake has been selected as one of the 59 biosphere reserves by UNESCO. This paper provides a comprehensive species checklist that needs to be updated by additional research in the future.

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Mitochondrial mediation of environmental osmolytes discrimination during osmoadaptation in the extremely halotolerant black yeast Hortaea werneckii

Cited by 29 publications

References 54 publications

Goa1p of Candida albicans Localizes to the Mitochondria during Stress and Is Required for Mitochondrial Function and Virulence

Goa1p of Candida albicans Localizes to the Mitochondria during Stress and Is Required for Mitochondrial Function and Virulence

Shedding Light on Selenium Biomineralization: Proteins Associated with Bionanominerals

Biodiversity of the Hypersaline Urmia Lake National Park (NW Iran)

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