Mitochondrial membrane potential

Zorova, Ljubava D.; Popkov, Vasily A.; Plotnikov, Egor Y.; Silachev, D. N.; Pevzner, Irina B.; Jankauskas, Stanislovas S.; Babenko, Valentina A.; Zorov, Savva D.; Balakireva, Anastasia V.; Juhaszova, Magdalena; Sollott, Steven J.; Zorov, Dmitry B.

doi:10.1016/j.ab.2017.07.009

Cited by 1,441 publications

(1,071 citation statements)

References 151 publications

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“…This gradient is facilitated by the activity of the ETC that generates a proton gradient across the inner mitochondrial membrane in which ATP synthase is embedded. MMP can be measured by flow cytometry using fluorescent dyes such as tetramethylrhodamine . mROS is generated when there is “leakage” of electrons from the ETC that can partially reduce oxygen to form superoxide (reviewed in Ref.…”

Section: An Introduction To Immunumetabolismmentioning

confidence: 99%

NK cell metabolism

Gardiner

2019

Journal of Leukocyte Biology

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Natural Killer (NK) cells are important antiviral and anticancer effector cells. They have excellent potential for immunotherapy although impaired functions during cancer limit their effectiveness. The discovery that cellular metabolism can impact on and regulate immune functions has led to an explosion of articles in this new area of immunometabolism. Metabolism has recently been shown to impact both murine and human NK cell biology. This review is targeted for newcomers to the field; it will introduce basic concepts in the area of immunometabolism including key aspects of glucose metabolism and mitochondrial function. It will review our current understanding of how metabolism of NK cells is differentially impacted in a variety of important situations. This is a rapidly expanding and exciting area of research that holds great potential for improving NK cell‐based immunotherapies.

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Section: An Introduction To Immunumetabolismmentioning

confidence: 99%

NK cell metabolism

Gardiner

2019

Journal of Leukocyte Biology

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“…Mitochondria play a key role in apoptosis, and early apoptosis often results in destruction of the mitochondrial membrane and loss of mitochondrial membrane potential . To determine whether Meriolin1 induces apoptosis of SH‐SY5Y cells through the mitochondrial pathway, the effect of Meriolin1 on the mitochondrial membrane potential was analysed after the cells were stained with Rhodamine 123.…”

Section: Resultsmentioning

confidence: 99%

“…Mitochondria play a key role in apoptosis, and early apoptosis often results in destruction of the mitochondrial membrane and loss of mitochondrial membrane potential. [27] To determine whether Meriolin1 induces apoptosis of SH-SY5Y cells through the mitochondrial pathway, the effect of Meriolin1 on the mitochondrial membrane potential was analysed after the cells were stained with Rhodamine 123. It can be seen from Figure 4a and 4b that after treatment with not the same concentrations of Meriolin1 (0.5, 1, 2 lM) for 24 h, the mitochondrial membrane potential decreased gradually with increasing drug concentration, showing a certain concentration dependence.…”

Section: Effect Of Meriolin1 On Mitochondrial Pathway-associated Protmentioning

confidence: 99%

Meriolin1 induces cell cycle arrest, apoptosis, autophagy and targeting the Akt/MAPKs pathways in human neuroblastoma SH-SY5Y cells

Wang

et al. 2020

Journal of Pharmacy and Pharmacology

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Objectives Meriolins, a kind of chemical hybrid between meridianins and variolins, have lately been determined as kinase inhibitors and reportedly have antitumour activity. However, there is currently no in‐depth study for the action mechanism. This study aimed to elucidate the potentially antitumour action mechanism of Meriolin1 on human neuroblastoma (SH‐SY5Y) cells. Methods Firstly, cell viability was detected by MTT assay. Secondly, cell cycle, cell apoptosis, cell autophagy, reactive oxygen species and mitochondrial membrane potential (ΔΨm) were measured by flow cytometry. Then, cell cycle‐associated proteins, Bcl‐2 family proteins, Akt/MAPK proteins and autophagy‐associated proteins expressions were evaluated by Western blot. Bcl‐2 and Bax mRNA expressions were also evaluated by qRT‐PCR. Furthermore, cell adhesion assay and Hoechst 33258 fluorescent staining were carried out to detect the effect of Meriolin1 on cell adhesion and morphology. Finally, to gain further insight into mechanism of action of Meriolin1 to CDK protein, the molecular docking study was performed by using the CDOCKER module of DS software. Key findings Meriolin1 could exert the antitumour activity on SH‐SY5Y cells by inducing cell cycle arrest, cell autophagy, the mitochondrion‐dependent cell apoptosis and targeting the Akt/MAPKs signalling pathway. Conclusions Meriolin1 might be a promising therapeutic candidate for neuroblastoma.

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“…This compromises the mitochondrial membrane integrity, leading to mitochondrial membrane depolarization and the release of pro‐apoptotic factors into the cytoplasm (Du & Yan, ; Li et al, ). The mitochondrial membrane potential is important for the synthesis of ATP and also for the maintenance of oxidative phosphorylation in the mitochondria (Zorova et al, ). Thus, the maintenance of mitochondrial function will be crucial in regulating apoptosis and providing energy in the form of ATP.…”

Section: Discussionmentioning

confidence: 99%

Chlorogenic acid‐rich Solanum melongena extract has protective potential against rotenone‐induced neurotoxicity in PC‐12 cells

Youn¹,

Jeon²,

Jin³

et al. 2019

J Food Biochem

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Neurodegenerative diseases are major threats to human health. Here, through fluorescence, colorimetric, immunoblotting, spectroscopy, and laser scanning confocal microscopic techniques, we investigated the neuroprotective properties of chlorogenic acid‐rich Solanum melongena extracts (SM extract) in rotenone‐induced PC‐12 cell death. The results showed that rotenone caused apoptosis to PC‐12 cells by elevating Bax/Bcl‐2 ratio and increasing caspase‐3 activity. Rotenone also increased ROS in cells while suppressing SOD and catalase activities. This resulted in the depletion of ATP in cells by blocking mitochondria complex I activity. Pretreatment of the cells with SM extract at concentrations of 100, 250, and 500 μg/ml before incubation for 24 hr with rotenone significantly prevented apoptosis, decreased ROS, and increased ATP production in the cells. SM extract upregulated SOD and catalase activities in the cells. These results unveil evidence that SM extract content neuroprotective properties that can be exploited to prevent and treat neurodegenerative diseases. Practical applications Solanum melongena eggplant is a popular ingredient in many traditional recipes and is well known in Asia for its medicinal benefits. Despite numerous scientific reports of the potential health benefits of this plant, reports on its effects in neurodegenerative diseases is still lacking. This pilot study demonstrates that S. melongena eggplant can protect against neurotoxicity in neurodegenerative diseases. The results of this research serves as a base for further research on eggplant that will result in its usage on a larger scale as functional food materials.

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Mitochondrial membrane potential

Cited by 1,441 publications

References 151 publications

NK cell metabolism

NK cell metabolism

Meriolin1 induces cell cycle arrest, apoptosis, autophagy and targeting the Akt/MAPKs pathways in human neuroblastoma SH-SY5Y cells

Chlorogenic acid‐rich Solanum melongena extract has protective potential against rotenone‐induced neurotoxicity in PC‐12 cells

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