Mitochondrial origins of the pressure to sleep

Abstract

SummaryThe neural control of sleep requires that sleep need is sensed during waking and discharged during sleep. To obtain a comprehensive, unbiased view of molecular changes in the brain that may underpin these processes, we have characterized the transcriptomes of single cells isolated from rested and sleep-deprived flies. Transcripts upregulated after sleep deprivation, in sleep-control neurons projecting to the dorsal fan-shaped body (dFBNs) but not ubiquitously in the brain, encode almost exclusively proteins with roles in mitochondrial respiration and ATP synthesis. These gene expression changes are accompanied by mitochondrial fragmentation, enhanced mitophagy, and an increase in the number of contacts between mitochondria and the endoplasmic reticulum, creating conduits for the replenishment of peroxidized lipids. The morphological changes are reversible after recovery sleep and blunted by the installation of an electron overflow in the respiratory chain. Inducing or preventing mitochondrial fission or fusion in dFBNs alters sleep and the electrical properties of sleep-control cells in opposite directions: hyperfused mitochondria increase, whereas fragmented mitochondria decrease, neuronal excitability and sleep. ATP levels in dFBNs rise after enforced waking because of diminished ATP consumption during the arousal-mediated inhibition of these neurons, which predisposes them to heightened oxidative stress. Consistent with this view, uncoupling electron flux from ATP synthesis relieves the pressure to sleep, while exacerbating mismatches between electron supply and ATP demand (by powering ATP synthesis with a light-driven proton pump) promotes sleep. Sleep, like ageing, may be an inescapable consequence of aerobic metabolism.

Circadian clock disruption engages the DREAM complex in suppressing cellular health

“…For instance, we found that mitochondrial and OXPHOS genes are downregulated by clock disruption and reinstated by co-inhibition of DREAM also in human cells (Figure 6E and 6F; Table S22), revealing mitochondria and OXPHOS as the most consistently clock-regulated entities across species and cell types. Interestingly, this observation is consistent with the recently discovered key involvement of mitochondria and OXPHOS in the induction of sleep and damage clearance during sleep 62,63 . Moreover, ribosome and ribosome biogenesis genes, and genes encoding the components of the transcriptional machinery showed opposite expression dynamics between clock-disrupted and DREAM co-inhibited cells (Figure S12D-F; Table S23).…”