Mitochondrial Oxygen Consumption by the Foreskin and its Fibroblast Rich Culture = إستهلاك الأوكسجين في المايتوكوندريا من قبل القلفة و الخلايا الليفية

OBJECTIVES: Whole-body hypothermia (to 33.5 ± 0.5 • C) is a therapeutic modality that reduces risks of death and neurodevelopmental disability in neonates subjected to hypoxic-ischemic insults. This in vitro study was designed to determine changes in neonatal cellular metabolism with temperature. Its main aim was to compare the metabolic rate at ≤33 • C with that at ≥35 • C. STUDY DESIGN: Foreskin specimens were used as a source of neonatal tissue. Cellular respiration (mitochondrial O 2 consumption) was used as a surrogate biomarker for the metabolic rate. Foreskin specimens from healthy newborns were collected immediately after circumcision and processed within one hour for measuring the rate of O 2 consumption at various temperatures (±0.5 • C). O 2 consumption was determined as function of time from the phosphorescence decay of Pd (II) meso-tetra-(4-sulfonatophenyl)-tetrabenzoporphyrin. RESULTS: In a vial sealed from air and containing foreskin specimen in phosphate-buffered saline supplemented with 5 mM glucose, [O 2 ] decreased linearly with time, confirming its zero-order kinetics. The rate of O 2 consumption (M O 2 .min −1 ), thus, was the negative of the slope of [O 2 ] vs. time. Cyanide inhibited O 2 consumption, confirming the oxidation occurred in the respiratory chain. Cellular respiration at ≤33 • C (n = 25) significantly differed from that at ≥35 • C (n = 24), p < 0.001. The rate (M O 2 .min −1 .mg −1 ) at 25 • C was 0.034 ± 0.006 (n = 11, p = 0.044), at 33 • C was 0.029 ± 0.008 (n = 14, reference temperature), at 35 • C was 0.062 ± 0.020 ( 2-fold higher, n = 18, p < 0.001), and at 37 • C was 0.061 ± 0.009 ( 2-fold higher, n = 6, p < 0.001). CONCLUSIONS: Neonatal foreskin cellular respiration is highly sensitive to critical temperatures (33 • C vs. 35 • C).

show abstract

Optimal temperature for whole-body hypothermia in the newborn: An in vitro study using foreskin mitochondrial oxygen consumption

Zaabi

Rahmani

Souid

2014

Journal of Neonatal-Perinatal Medicine

View full text Add to dashboard Cite

show abstract

In Vitro Study on the Response of Fibroblast Cellular Respiration to Lipoic acid, Thiamine and Carnitine in Patients with Dihydrolipoyl Dehydrogenase Deficiency

Al-Jasmi

Pramathan²,

Kowash³

et al. 2016

J Mol Genet Med

Self Cite

View full text Add to dashboard Cite

Objectives: This study examined in vitro responses of fibroblast cellular respiration to lipoic acid, thiamine and carnitine in patients with dihydrolipoyl dehydrogenase (DLD) deficiency. This disorder impairs cellular bioenergetics and these compounds are used to improve clinical manifestations of the disease. The study aimed to utilize mitochondrial O 2 consumption as a surrogate biomarker for examining cellular responses to metabolic therapies.Methods: Cultured fibroblasts from three patients were treated with therapeutic concentrations of the compounds for 24 hours. Cells were then harvested and processed for measuring respiration using phosphorescence oxygen analyzer. Patients 1 and 2 were severely symptomatic infants with homozygous c.1436A>T mutation in the DLD gene. Patient-3 was a mildly symptomatic adolescent with homozygous c.685G>T mutation. Results:The rate of respiration (mean ± SD, n=6, µM O 2 min -1 /10 7 cells) in fibroblasts from a normal infant was 9.3 ± 1.6, in fibroblasts from Patient-1 was 5.1 ± 0.9 (p=0.001), in fibroblasts from Patient-2 was 7.4 ± 1.4 (p=0.051), and in fibroblasts from Patient-3 was 10.3 ± 3.3 (p=0.836). In normal fibroblasts, respiration decreased by the thiamine (p=0.012) and carnitine (p=0.023) treatments. In Patient-1, respiration increased by the lipoic acid (p<0.002), thiamine (p<0.001), and carnitine (p=0.018) treatments; this patient clinically responded to thiamine. In Patient-2, respiration decreased by the thiamine (p=0.026) and carnitine (p=0.008) treatments; this patient did not respond to these drugs. In Patient-3, respiration increased by the carnitine (p=0.012) treatment; the patient clinically responded to carnitine. Conclusions:The results show cellular respiration is a suitable biomarker for the disease. The significance of using this tool to assess responses to therapies requires further studies.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Mitochondrial Oxygen Consumption by the Foreskin and its Fibroblast Rich Culture = إستهلاك الأوكسجين في المايتوكوندريا من قبل القلفة و الخلايا الليفية

Cited by 2 publications

References 13 publications

Optimal temperature for whole-body hypothermia in the newborn: An in vitro study using foreskin mitochondrial oxygen consumption

Optimal temperature for whole-body hypothermia in the newborn: An in vitro study using foreskin mitochondrial oxygen consumption

In Vitro Study on the Response of Fibroblast Cellular Respiration to Lipoic acid, Thiamine and Carnitine in Patients with Dihydrolipoyl Dehydrogenase Deficiency

Contact Info

Product

Resources

About