Mitochondrial phylogeny of the Chrysis ignita (Hymenoptera: Chrysididae) species group based on simultaneous Bayesian alignment and phylogeny reconstruction

Soon, Villu; Saarma, Urmas

doi:10.1016/j.ympev.2011.04.005

Cited by 18 publications

(22 citation statements)

References 17 publications

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“…Nevertheless, the specific status of this species has never been under question. We based outgroup selection on the study by Soon & Saarma (2011), selecting one distantly related species (C. indigotea Dufour & Perris, 1840) from the same species group and one representative of another closely related species group, namely C. graelsii Guérin-Meneville, 1842 from the C. graelsii species group. Both air-dried specimens from collections and fresh material collected and stored in 96% ethanol were used to extract genomic DNA.…”

Section: Chrysis Terminata Dahlbom 1854mentioning

confidence: 99%

“…Both air-dried specimens from collections and fresh material collected and stored in 96% ethanol were used to extract genomic DNA. In addition to 35 newly sequenced specimens, 16 previously published (Soon & Saarma 2011) sequences from Northern European species were included in this analysis. Altogether, 51 specimens from 12 European countries were included in this analysis; these belonged to 16 ingroup species (plus three additional specimens of unknown identity) and two outgroup species.…”

Section: Chrysis Terminata Dahlbom 1854mentioning

confidence: 99%

“…PCR conditions and primers to amplify 12S and 16S rRNA genes, tRNA Val and a small fragment of the ND4 gene of mtDNA were exactly as described in Soon & Saarma (2011); primers used for amplification of a fragment of COI are given in Table 2. After PCR the solution was treated with shrimp alkaline phosphatase and exonuclease I (USB, Cleveland, USA).…”

Section: Chrysis Terminata Dahlbom 1854mentioning

confidence: 99%

“…Such studies have included phylogenetic reconstructions of the entire Hymenoptera (Carpenter & Wheeler 1999), suborder Apocrita (Dowton & Austin 2001;Pilgrim et al 2008), superfamily Apoidea (Ohl & Bleidorn 2006) and family Bethylidae (Carr et al 2010). Molecular methods have been used to directly study Chrysididae in a few instances (Niehuis & Wägele 2004;Niehuis & Korb 2010;Soon & Saarma 2011) but none of these have attempted to delimit species. While the study by Soon & Saarma (2011) that dealt with the phylogeny of the C. ignita species group suggested some taxonomical changes, it did not assess the distinctness or species rank of each included species.…”

Section: Introductionmentioning

confidence: 99%

“…Molecular methods have been used to directly study Chrysididae in a few instances (Niehuis & Wägele 2004;Niehuis & Korb 2010;Soon & Saarma 2011) but none of these have attempted to delimit species. While the study by Soon & Saarma (2011) that dealt with the phylogeny of the C. ignita species group suggested some taxonomical changes, it did not assess the distinctness or species rank of each included species. As only single specimens of each species or subspecies were included in that study, it was only possible to suggest elevation of subspecies to species rank in cases where different subspecies of the same species did not form monophyletic group.…”

Section: Introductionmentioning

confidence: 99%

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Testing the validity of Northern European species in the Chrysis ignita species group (Hymenoptera: Chrysididae) with DNA Barcoding

Soon¹,

Budrys²,

Orlovskytė³

et al. 2014

Zootaxa

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Containing more than a hundred species, the Chrysis ignita species group is the largest and one of the most taxonomically challenging groups in its genus. It has not been possible to resolve the taxonomy of the group using traditional methods due to the lack of robust diagnostic morphological characters. Here we present the results of a molecular analysis designed to delimit species in the Chrysis ignita group for the first time; using mitochondrial sequence data for 364 in-group specimens consisting of all 18 species known to occur in Northern Europe. Two mitochondrial loci were analysed: a COI gene fragment, and a continuous DNA sequence consisting of 16S rRNA, tRNAVal , 12S rRNA and ND4. Two approaches were employed for delimiting species: (1) genetic distance analysis based on the standard COI barcode sequences and; (2) phylogenetic analysis of the COI fragment together with rRNA genes. Both analyses yielded trees with similar topology, but support values for nodes were higher using the second approach. Fifteen species were distinguished in all analyses: Chrysis angustula Schenck, 1856, C. brevitarsis Thomson, 1870, C. clarinicollis Linsenmaier, 1951, C. corusca Valkeila, 1971, C. fulgida Linnaeus, 1761, C. ignita (Linnaeus, 1758), C. impressa Schenck, 1856, C. iris Christ, 1791, C. leptomandibularis Niehuis, 2000, C. longula Abeille de Perrin, 1879, C. ruddii Shuckard, 1837, C. schencki Linsenmaier, 1968, C. subcoriacea Linsenmaier, 1959, C. terminata Dahlbom, 1854 and C. vanlithi Linsenmaier, 1959. The specific status of C. mediata Linsenmaier, 1951 and C. solida Haupt, 1957 was not resolved. Included unidentified specimens grouped in three clusters, two of which are distinctly delimited and apparently represent cryptic species. The specific status of the unidentified samples in the third cluster remained unclear. Moreover, our data suggest the existence of additional cryptic species currently lumped under the names C. pseudobrevitarsis Linsenmaier, 1951 and C. schencki Linsenmaier, 1968. In conclusion, our results derived from analysis of mitochondrial loci strongly support the specific status of the majority of currently recognised species in the Chrysis ignita species group, and suggest the existence of additional cryptic species in Northern Europe. Thus, considering the difficulties that often arise during species determination based on morphological characters, the mtDNA loci used here appear highly suitable for assisting species delimitation in this group as well as identification of specimens.

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Section: Chrysis Terminata Dahlbom 1854mentioning

confidence: 99%

Section: Chrysis Terminata Dahlbom 1854mentioning

confidence: 99%

Section: Chrysis Terminata Dahlbom 1854mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Testing the validity of Northern European species in the Chrysis ignita species group (Hymenoptera: Chrysididae) with DNA Barcoding

Soon¹,

Budrys²,

Orlovskytė³

et al. 2014

Zootaxa

Self Cite

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Dynamics of the abundance of cuckoo wasps (Hymenoptera, Chrysididae) in the conditions of the altitudinal-belt heterogeneity of the North Caucasus

Vinokurov

2021

BIO Web Conf.

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The work provides data on the dynamics of the abundance of 205 taxa from 19 genera: Cleptes, Colpopyga, Elampus, Omalus, Philoctetes, Pseudomalus, Hedychridium, Hedychrum, Holopyga, Chrysidea, Chrysis, Euchroeus, Chrysura, Pseudochrysis, Spinolia, Spinolia, including species rare and new for the fauna of the North Caucasus and Russia. By the nature of the population dynamics, five groups of cuckoo wasps were identified: spring – represented by 7 species from 4 genera; spring-summer – 76 species from 10 genera; summer – 113 species from 16 genera; summer-autumn – 4 species from 2 genera and spring-summer-autumn – 5 species from 3 genus. According to the duration of flight, eurychronous species of cuckoo wasps were identified, which are found throughout the season from spring to autumn; mesochronous – the years of which affect the end of May and summer months and stenochronous – confined to a short period of time. Eurychronic accounted for 2.4%, mesochronous 24.9%, stenochronic 72.7%. The number of generations of cuckoo wasps is related to the dynamics of the number of the host. In the mountains, the peaks of activity shift towards the middle of summer, most species have one peak of activity; due to the frequent changes in weather conditions in the mountains, the phenological characteristics of the cuckoo wasps and their hosts do not coincide in terms with the lowland populations and the periods of activity can be extended in time. Phenological characteristics of rare and new species for the fauna of the North Caucasus and Russia are presented for the first time. The data obtained expand the understanding of the biodiversity and dynamics of the abundance of cuckoo wasps in the North Caucasus and can be used for environmental protection measures and rational nature management in the south of Russia.

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A journey through the history of the British Chrysididae (Hymenoptera): unexpected taxonomic problems, new records and description of a new species

Rosa

2024

Journal of Natural History

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Mitochondrial phylogeny of the Chrysis ignita (Hymenoptera: Chrysididae) species group based on simultaneous Bayesian alignment and phylogeny reconstruction

Cited by 18 publications

References 17 publications

Testing the validity of Northern European species in the Chrysis ignita species group (Hymenoptera: Chrysididae) with DNA Barcoding

Testing the validity of Northern European species in the Chrysis ignita species group (Hymenoptera: Chrysididae) with DNA Barcoding

Dynamics of the abundance of cuckoo wasps (Hymenoptera, Chrysididae) in the conditions of the altitudinal-belt heterogeneity of the North Caucasus

A journey through the history of the British Chrysididae (Hymenoptera): unexpected taxonomic problems, new records and description of a new species

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