Mitochondrial proteome profiling of Leishmania tropica

Tasbihi, Minoo; Shekari, Faezeh; Hajjaran, Homa; Masoori, Leila; Hadighi, Ramtin

doi:10.1016/j.micpath.2019.103542

Cited by 12 publications

(9 citation statements)

References 51 publications

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“…Mitochondrion is a cellular organelle in which molecular chaperones are of particular relevance because they are involved in protein transport across membranes and protein refolding inside the mitochondria. Recently, the mitochondrial proteome was analyzed in L. tropica [22]. Taking advantage of that study, in Table 3, we list those HSPs identified in the L. infantum proteome that are potentially mitochondrial proteins.…”

Section: Components Of the Proteostasis Networkmentioning

confidence: 99%

“…Thus, proteomics approaches have been used to determine differential patterns of protein expression between the promastigote and amastigote stages in Leishmania mexicana [18], L. infantum [19], and L. donovani [20], among others. Other studies have been aimed to ascertain specific proteomes by means of organelle fractionation to obtain enriched fractions of mitochondria, flagella, or glycosomes [21,22]. The identification and mapping of protein post-translational modifications (PTMs) provide additional information about the activation of specific pathways in a given growing condition, thus improving the knowledge on protein interactions in complex networks.…”

Section: Introductionmentioning

confidence: 99%

“…Identified molecular chaperones of L. infantum promastigotes, with putative mitochondrial location (according to Tasbihi et al[22]). …”

mentioning

confidence: 99%

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The Experimental Proteome of Leishmania infantum Promastigote and Its Usefulness for Improving Gene Annotations

Sanchiz

Morato

Rastrojo

et al. 2020

Genes

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Leishmania infantum causes visceral leishmaniasis (kala-azar), the most severe form of leishmaniasis, which is lethal if untreated. A few years ago, the re-sequencing and de novo assembling of the L. infantum (JPCM5 strain) genome was accomplished, and now we aimed to describe and characterize the experimental proteome of this species. In this work, we performed a proteomic analysis from axenic cultured promastigotes and carried out a detailed comparison with other Leishmania experimental proteomes published to date. We identified 2352 proteins based on a search of mass spectrometry data against a database built from the six-frame translated genome sequence of L. infantum. We detected many proteins belonging to organelles such as glycosomes, mitochondria, or flagellum, as well as many metabolic enzymes and many putative RNA binding proteins and molecular chaperones. Moreover, we listed some proteins presenting post-translational modifications, such as phosphorylations, acetylations, and methylations. On the other hand, the identification of peptides mapping to genomic regions previously annotated as non-coding allowed for the correction of annotations, leading to the N-terminal extension of protein sequences and the uncovering of eight novel protein-coding genes. The alliance of proteomics, genomics, and transcriptomics has resulted in a powerful combination for improving the annotation of the L. infantum reference genome.

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Section: Components Of the Proteostasis Networkmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Experimental Proteome of Leishmania infantum Promastigote and Its Usefulness for Improving Gene Annotations

Sanchiz

Morato

Rastrojo

et al. 2020

Genes

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“…Mitochondrion is a cellular organelle in which molecular chaperones are of particular relevance as they are involved in the protein transport across membranes and protein refolding inside the mitochondria. The mitochondrial proteome has been recently analyzed in L. tropica [22]. Taking advantage of that study, we are listing in Table 3 those HSPs identified in the L. infantum proteome that are candidate to be mitochondrial proteins.…”

Section: Components Of the Proteostasis Networkmentioning

confidence: 99%

“…Thus, different proteomic approaches have been used to determine differential patterns of protein expression between the promastigote and amastigote stages in L. mexicana [18], L. infantum [19], and L. donovani [20], among others. Other studies were aimed to ascertain specific proteomes by means of organelle fractionation to obtain enriched fractions of mitochondria, flagella or glycosomes [21,22]. The identification and mapping of protein post-translational modifications (PTMs) provide additional information about activation of specific pathways in a given growing condition, improving the knowledge on protein interactions in complex networks.…”

Section: Introductionmentioning

confidence: 99%

The Experimental Proteome of Leishmania infantum promastigote and Its Usefulness for Improving Gene Annotations

Sanchiz¹,

Morato²,

Rastrojo³

et al. 2020

Preprint

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Leishmania infantum is causative of visceral leishmaniasis (kala-azar), the most severe form of leishmaniasis, lethal if untreated. Few years ago, re-sequencing and de novo assembling of the L. infantum (strain JPCM5) genome was accomplished, and now we aimed to describe and characterize the experimental proteome of this species. In this work, we have performed a proteomic analysis from axenic cultured promastigotes and carried out a detailed comparison with other Leishmania experimental proteomes published to date. We identified 2,352 proteins based on the search of mass spectrometry data against a database built from the six-frame translated genome sequence of L. infantum. We have detected many proteins belonging to organelles such as glycosomes, mitochondria or flagellum, as well as many metabolic enzymes, and a large number of putative RNA binding proteins and molecular chaperones. Moreover, we listed the proteins presenting post-translational modifications, such as phosphorylations, acetylations and methylations, among others. On the other hand, the identification of peptides mapping to genomic regions previously annotated as non-coding has allowed to correct annotations, leading to N-terminal extension of protein sequences, and the uncovering of eight novel protein-coding genes. The alliance of proteomics, genomics and transcriptomics has resulted in a powerful combination for improving the L. infantum reference genome annotation.

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Proteomic profiling of mesenchymal stem cell‐derived extracellular vesicles: Impact of isolation methods on protein cargo

Abyadeh,

Mirshahvaladi,

Kashani

et al. 2024

J of Extracellular Bio

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Extracellular vesicles (EVs) are nanosized vesicles with a lipid bilayer that are secreted by cells and play a critical role in cell‐to‐cell communication. Despite the promising reports regarding their diagnostic and therapeutic potential, the utilization of EVs in the clinical setting is limited due to insufficient information about their cargo and a lack of standardization in isolation and analysis methods. Considering protein cargos in EVs as key contributors to their therapeutic potency, we conducted a tandem mass tag (TMT) quantitative proteomics analysis of three subpopulations of mesenchymal stem cell (MSC)‐derived EVs obtained through three different isolation techniques: ultracentrifugation (UC), high‐speed centrifugation (HS), and ultracentrifugation on sucrose cushion (SU). Subsequently, we checked EV marker expression, size distribution, and morphological characterization, followed by bioinformatic analysis. The bioinformatic analysis of the proteome results revealed that these subpopulations exhibit distinct molecular and functional characteristics. The choice of isolation method impacts the proteome of isolated EVs by isolating different subpopulations of EVs. Specifically, EVs isolated through the high‐speed centrifugation (HS) method exhibited a higher abundance of ribosomal and mitochondrial proteins. Functional apoptosis assays comparing isolated mitochondria with EVs isolated through different methods revealed that HS‐EVs, but not other EVs, induced early apoptosis in cancer cells. On the other hand, EVs isolated using the sucrose cushion (SU) and ultracentrifugation (UC) methods demonstrated a higher abundance of proteins primarily involved in the immune response, cell‐cell interactions and extracellular matrix interactions. Our analyses unveil notable disparities in proteins and associated biological functions among EV subpopulations, underscoring the importance of meticulously selecting isolation methods and resultant EV subpopulations based on the intended application.

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Mitochondrial proteome profiling of Leishmania tropica

Cited by 12 publications

References 51 publications

The Experimental Proteome of Leishmania infantum Promastigote and Its Usefulness for Improving Gene Annotations

The Experimental Proteome of Leishmania infantum Promastigote and Its Usefulness for Improving Gene Annotations

The Experimental Proteome of Leishmania infantum promastigote and Its Usefulness for Improving Gene Annotations

Proteomic profiling of mesenchymal stem cell‐derived extracellular vesicles: Impact of isolation methods on protein cargo

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