Mitochondrial reactive oxygen species and nitric oxide-mediated cancer cell apoptosis in 2-butylamino-2-demethoxyhypocrellin B photodynamic treatment

Lu, Zhigang; Tao, Yi; Zhou, Zhixiang; Zhang, Junjing; Liu, Cong; Ou, Lingcheng; Zhao, Baolu

doi:10.1016/j.freeradbiomed.2006.08.021

Cited by 54 publications

(75 citation statements)

References 68 publications

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“…PTIO (20.0 mol/l), as a NO scavenger, could reduce NO level of cells (Lu et al, 2006). PBDE-47 increased cells apoptosis dosedependently, whereas PTIO reversed the cell apoptosis (Fig.…”

Section: Cell Apoptosis Induced By Pbde-47 and Hbcds Individually Or mentioning

confidence: 87%

Effects of tetrabrominated diphenyl ether and hexabromocyclododecanes in single and complex exposure to hepatoma HepG2 cells

2009

Environmental Toxicology and Pharmacology

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“…PTIO (20.0 mol/l), as a NO scavenger, could reduce NO level of cells (Lu et al, 2006). PBDE-47 increased cells apoptosis dosedependently, whereas PTIO reversed the cell apoptosis (Fig.…”

Section: Cell Apoptosis Induced By Pbde-47 and Hbcds Individually Or mentioning

confidence: 87%

Effects of tetrabrominated diphenyl ether and hexabromocyclododecanes in single and complex exposure to hepatoma HepG2 cells

2009

Environmental Toxicology and Pharmacology

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“…• ), hydrogen peroxide (H 2 O 2 ), hydroxyl radical ( • OH), and type II ROS such as singlet oxygen have been implicated in the therapeutic or toxic responses of PDT (3). PDT produces cytotoxic effects through photodamage to subcellular organelles and molecules such as mitochondria, lysosomes, cell membranes, and nuclei of tumor cells, which are considered as potential targets.…”

Section: Introductionmentioning

confidence: 99%

Efficient preparation of highly pure chlorin e6 and its photodynamic anti-cancer activity in a rat tumor model

Ahn¹

2009

Oncol Rep

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Abstract. Photodynamic therapy (PDT) is currently being used as an alternative therapeutic modality for a variety of malignant tumors. This study was performed to show an efficient preparation of second generation of photosensitizer chlorin e 6 (Ce 6 ) with high yield and purity, and to test antitumor activity of Ce 6 -induced PDT (Ce 6 -PDT) both in vitro and in vivo using a rat tumor model. Three-week-old male Sprague-Dawley (SD) rats were inoculated s.c. on the right flank with 5x10 6 RK3E-ras cells. The animals were administered i.v. with Ce 6 (10 mg/kg) and 24 h later, PDT was performed using a laser diode at a light dose of 100 J/cm 2 . Ce 6 -PDT generated reactive oxygen species and led to significant growth inhibition in RK3E-ras cell. In addition, Ce 6 -PDT induced apoptosis through the activation of caspase-3 and its downstream target, PARP cleavage. The protein level of antiapoptotic bcl-2 was also reduced by Ce 6 -PDT in RK3E-ras cells. In in vivo experiments, application of Ce 6 -PDT led to a significant reduction of tumor size. PCNA immunostaining and TUNEL assay revealed that Ce 6 -PDT inhibited tumor cell proliferation and increased apoptosis. These findings suggest that the newly purified Ce 6 -PDT can effectively arrest tumor growth by inhibiting cell proliferation and inducing apoptosis.

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“…Antioxidants, on the other hand, are primarily reducing agents prone to scavenge free radicals; consequently, antioxidants could compete with oxygen for quenching of the triplet sensitizer or can neutralize the generated reactive species, thus counteracting the effect of PDT [40][41][42].…”

Section: Discussionmentioning

confidence: 99%

Modulation of 5-Aminolevulinic acid mediated photodynamic therapy induced cell death in a human lung adenocarcinoma cell line

Mj¹,

Diez²,

Batlle³

et al. 2016

Integr Cancer Sci Therap

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Photodynamic therapy (PDT) is a cancer treatment involving the administration of a photosensitising drug which selectively accumulates in tumor tissue, followed by irradiation with appropriate wavelength light. It triggers photochemical reactions inducing reactive oxygen species (ROS) production with the consequent cellular damage, which ultimately leads to cell death. Porphyrins are the only photosensitizers (PSs) endogenously synthesized by means of administration of the biological precursor, 5-aminolevulinic acid (ALA). Several antioxidants and ROS scavenger agents: reduced glutathione (GSH), mannitol (Man), l-tryptophan (Trp), ascorbate (Asc) and trolox (Trx), were assayed to determine their ability to modulate ALA-based PDT (ALA-PDT); it was performed on A549 human lung adenocarcinoma cells, by incubating with 1mM ALA for 3 hr and followed by irradiation with or without 1 hr pre-incubation with the modulators. They were previously tested for possible cytotoxicity/ photoactivity in concentrations ranging from 0.01 to 20 mM. The ratio between cell survival after ALA-PDT in the presence and in the absence of the scavenger agent (protection grade: PG) was determined, and the concentration showing no cytotoxicity/ photoactivity and providing the highest PG was used in the subsequent experiments. ALA-PDT alone induced a high percentage of apoptotic cell death (98.4 ± 3.5%) as revealed by acridine orange/ethidium bromide staining and AnnexinV-FITC/propidium iodide labelling. Pre-incubation with the modulators at their highest PG concentration significantly reduced apoptotic cells to 48.3 ± 2.7% (Asc), 58.8 ± 4.2 (Trx), 78.5 ± 3.1% (GSH), 64.3 ± 1.6% (Man), 74.6 ± 2.3% (Trp). ROS involvement in early cell death induction after ALA-PDT was tested by flow cytometry using the fluorescent probes dihydro-dichlorofluorescein diacetate (H2-DCFDA) and methoxyvinylpyrene (MVP) for detection of peroxides and singlet oxygen, respectively. ROS production increased after ALA-PDT (H2-DCFDA positive cells, control: 1.1 ± 0.1 %; 10 min-PDT: 69.3 ± 5.6%; MVP positive cells, control: 0.65 ± 0.35%; 10 min-PDT: 83.5 ± 1.9%). Asc prevented peroxide formation (H2-DCFDA positive cells: 50.7 ± 2.8%) and mostly prevented singlet oxygen increase (MVP positive cells: 25.4 ± 5.2%) whereas Trx limited peroxides formation (H2-DCFDA positive cells: 20.8 ± 0.5%), but did not significantly affected singlet oxygen production (MVP positive cells: 73.6 ± 3.4%). Selective scavenger mediated protection against PDT-induced cell death, and direct detection of specific pro-oxidative agents, entail the strong involvement of ROS in ALA-PDT-mediated tumor eradication, suggesting that undesired photodamage to normal tissue might be attenuated by administration of antioxidant agents.

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Mitochondrial reactive oxygen species and nitric oxide-mediated cancer cell apoptosis in 2-butylamino-2-demethoxyhypocrellin B photodynamic treatment

Cited by 54 publications

References 68 publications

Effects of tetrabrominated diphenyl ether and hexabromocyclododecanes in single and complex exposure to hepatoma HepG2 cells

Effects of tetrabrominated diphenyl ether and hexabromocyclododecanes in single and complex exposure to hepatoma HepG2 cells

Efficient preparation of highly pure chlorin e6 and its photodynamic anti-cancer activity in a rat tumor model

Modulation of 5-Aminolevulinic acid mediated photodynamic therapy induced cell death in a human lung adenocarcinoma cell line

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