1999
DOI: 10.1128/mcb.19.5.3842
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Mitochondrial Regulation of Cell Death: Mitochondria Are Essential for Procaspase 3-p21 Complex Formation To Resist Fas-Mediated Cell Death

Abstract: Death receptor Fas transduces cell death signaling upon stimulation by Fas ligand, and this death signaling is mediated by caspase. Recently, we reported that the cell cycle regulator p21 interacts with procaspase 3 to resist Fas-mediated cell death. In the present study, the molecular characterization and functional region of the procaspase 3-p21 complex was further investigated. We observed the p21 expression in the mitochondrial fraction of HepG2 cells and detected Fas-mediated cell death only in the presen… Show more

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Cited by 117 publications
(108 citation statements)
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“…34 p21 Cip1 can relocate to the mitochondria, where it interacts with procaspase-3 to inhibit caspase 3 activation and to resist Fas death receptor-induced cell death. 32,36 Finally, p57 Kip2 expression appears to facilitate Bax activation and translocation to the mitochondria upon STS treatment. Whether the translocation of p57 Kip2 to the mitochondria is directly related to the activation of Bax activation still awaits additional experiments.…”
Section: Discussionmentioning
confidence: 99%
“…34 p21 Cip1 can relocate to the mitochondria, where it interacts with procaspase-3 to inhibit caspase 3 activation and to resist Fas death receptor-induced cell death. 32,36 Finally, p57 Kip2 expression appears to facilitate Bax activation and translocation to the mitochondria upon STS treatment. Whether the translocation of p57 Kip2 to the mitochondria is directly related to the activation of Bax activation still awaits additional experiments.…”
Section: Discussionmentioning
confidence: 99%
“…The DNA precipitate was resuspended, and electrophoresed in a 2% agarose gel. Morphological changes in the nuclear chromatin of HCC cells undergoing apoptosis were detected by staining with Hoechst 33342 (Wako, Osaka, Japan) (Suzuki et al, 1999). One thousand cells were observed in five randomly chosen areas for each sample.…”
Section: Cell Proliferation and Apoptosismentioning
confidence: 99%
“…Subcellular fractionation of cytosol and mitochondria was performed according to the method described previously (Suzuki et al, 1999). Briefly, harvested cells were suspended in a hypotonic buffer (250 mM sucrose, 20 mM HEPES/KOH pH 7.5, 1.5 mM MgCl 2 , 10mM KCl, 1 mM EDTA, 1 mM EGTA, 1 mM dithiothreitol, 1 mM PMSF, 10 mg/ml aprotinin, 10 mg/ml leupetin, and 1.8 mg/ml iodoacetamide), passed through a 25-gauge needle and centrifuged at 1000 r.p.m.…”
Section: Subcellular Fractionation Of Cellsmentioning
confidence: 99%
“…The role of p21 Cip1 in lymphocytes is less clear. Various reports suggest that p21 Cip1 may act to inhibit apoptosis (8), promote cell cycle withdrawal upon prolonged stimulation (9), or promote apoptosis (10). More recent data suggest that p21 Cip1 plays a role in Fas-mediated activation-induced cell death (11,12).…”
mentioning
confidence: 99%