Mitochondrial Ribosomal Protein L14 Promotes Cell Growth and Invasion by Modulating Reactive Oxygen Species in Thyroid Cancer

Abstract

Objectives. The mitochondrial ribosomal protein L14 (MRPL14) is encoded by a nuclear gene and participates in mitochondrial protein translation. In this study, we aimed to investigate the role of MRPL14 in thyroid carcinoma. Methods. We investigated the association of expression of MRPL14 and clinicopathological features using the The Cancer Genome Atlas (TCGA) and Chungnam National University Hospital (CNUH) databases. Functional studies of MRPL14, including proliferation, migration, invasion, mitochondrial oxidative phosphorylation and reactive oxygen species (ROS) production, were performed in papillary thyroid carcinoma (PTC) cell lines (B-CPAP and KTC-1).Results. Based on TCGA dataset, PTC tissues lost mitochondrial integrity and showed dysregulated expression of overall mitoribosomal proteins (MRPs) compared with normal thyroid tissues. Of 78 MRPs, MRPL14 was highly expressed in thyroid carcinoma tissues. MRPL14 overexpression was significantly associated with advanced tumor stage, extrathyroidal extension, and lymph node metastasis. MRL14 increased cell proliferation of thyroid cancer and promoted cell migration via epithelial-mesenchymal transition-related proteins. Moreover, MRPL14 knockdown reduced the expression of oxidative phosphorylation complex IV (MTCO1) and increased the accumulation of ROS. Co-treatment with a ROS scavenger restored cell proliferation and migration reduced by MRPL14 knockdown, which imply that ROS functions as a key regulator of the oncogenic effects of MRPL14 in thyroid cancer cellsConclusion. Our findings indicate that MRPL14 may promote cell growth, migration, and invasion through modulating ROS in thyroid cancer cells.

Mitochondrial ribosomal protein L14 (MRPL14) significantly correlated with decreased risk of endometrioid endometrial cancer: A two-sample Mendelian randomization study

“…Being interacted with C7orf30, MRPL14 enhanced the biogenesis of the mitochondrial large ribosomal subunit and promoted mitochondrial translation [40] . A recent study found high expression of MRPL14 in thyroid cancer tissues compared with health control, whereas MRPL14 overexpression signi cantly correlated with aggressive clinical characteristics such as advanced stage, extrathyroidal extension, and lymph node metastasis [41] . MRPL14 knockdown resulted in accumulation of reactive oxygen species (ROS), whereas ROS scavenger treatment restored tumor cell proliferation and migration, suggesting that MRPL14 might modulate ROS to accelerate the progression and metastasis of thyroid cancer [41] .…”

“…A recent study found high expression of MRPL14 in thyroid cancer tissues compared with health control, whereas MRPL14 overexpression signi cantly correlated with aggressive clinical characteristics such as advanced stage, extrathyroidal extension, and lymph node metastasis [41] . MRPL14 knockdown resulted in accumulation of reactive oxygen species (ROS), whereas ROS scavenger treatment restored tumor cell proliferation and migration, suggesting that MRPL14 might modulate ROS to accelerate the progression and metastasis of thyroid cancer [41] . In addition, MRPL14 expression was found signi cantly higher in Helicobacter pylori related gastric cancer compared with health groups [42] .…”

Role of oxidative stress and IL-17 induced inflammatory cytokines in thyroid carcinoma with Hashimoto's thyroiditis

“…Oxidative stress not only participates in tumor formation and development, but also leads to cancer cell death. The accumulation of reactive oxygen species can also lead to migration and invasion of cancer cells [5]. , also known as IL-17A, is a highly versatile pro-in ammatory cytokine that is critical to a variety of processes, including host defense, tissue repair, pathogenesis of in ammatory diseases, and cancer progression [6][7].There have been few reports on the expression changes and interaction characteristics of oxidative stress and IL-17-driven in ammatory factors in thyroid cancer with HT.In this study, oxidative stress indexes such as NO, eNOS, SOD, and in ammatory indexes such as IL-17A, IL-1β and IL-6 were analyzed in patients with thyroid cancer and Hashimoto thyroiditis, and their correlation and possible diagnostic role were discussed in order to further clarify the occurrence and development mechanism of thyroid cancer with Hashimoto thyroiditis.…”