2015
DOI: 10.1016/j.celrep.2015.01.030
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Mitochondrial RNA Granules Are Centers for Posttranscriptional RNA Processing and Ribosome Biogenesis

Abstract: Cytoplasmic RNA granules play a central role in mRNA metabolism, but the importance of mitochondrial RNA granules remains relatively unexplored. We characterized their proteome and found that they contain a large toolbox of proteins dedicated to RNA metabolism. Investigation of four uncharacterized putative RNA-binding proteins-two RNA helicases, DHX30 and DDX28, and two proteins of the Fas-activated serine-threonine kinase (FASTKD) family, FASTKD2 and FASTKD5-demonstrated that both helicases and FASTKD2 are r… Show more

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Cited by 266 publications
(328 citation statements)
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“…3E, F). Fully consistent with the results reported here, depletion of FASTKD2 by RNAi was just found in to cause misassembly of the mitochondrial ribosome and impaired mitochondrial protein synthesis in human immortalized fibroblasts (Antonicka and Shoubridge 2015). Our observations for FASTKD2 also complement a recent report implicating RAP, a protein of Arabidopsis thaliana that shares the RAP domain with FASTKD2, in precursor processing of the small ribosomal subunit RNA in chloroplasts (Kleinknecht et al 2014).…”
Section: Depletion Of Fastkd2 Affects the Levels Of Several Of Its Tasupporting
confidence: 81%
See 1 more Smart Citation
“…3E, F). Fully consistent with the results reported here, depletion of FASTKD2 by RNAi was just found in to cause misassembly of the mitochondrial ribosome and impaired mitochondrial protein synthesis in human immortalized fibroblasts (Antonicka and Shoubridge 2015). Our observations for FASTKD2 also complement a recent report implicating RAP, a protein of Arabidopsis thaliana that shares the RAP domain with FASTKD2, in precursor processing of the small ribosomal subunit RNA in chloroplasts (Kleinknecht et al 2014).…”
Section: Depletion Of Fastkd2 Affects the Levels Of Several Of Its Tasupporting
confidence: 81%
“…Thus, selective depletion of RNR2 may occur due to its aberrant processing or due to ribosome misassembly. Indeed, recent studies suggest that failure to assemble RNR2 with mitochondrial ribosomal proteins leads to its selective depletion, which has been proposed to be mediated by the mitochondrial degradosome (Antonicka and Shoubridge 2015;Tu and Barrientos 2015). The levels of isoleucyl tRNA (TRNI), one of the heavystrand-encoded tRNAs highly enriched in FASTKD2-GFP libraries, are unaffected by depletion of FASTKD2 by RNAi and CRISPR/Cas9-mediated mutagenesis (Fig.…”
Section: Depletion Of Fastkd2 Affects the Levels Of Several Of Its Tamentioning
confidence: 99%
“…These results were confirmed in cultured human cells although biochemical studies localized most of the protein in mitochondria [127]. DDX28 interacts with the 16S rRNA and the mtLSU [127], most probably with a helix in domain V [128]. RNAi-mediated DDX28 silencing in HEK293T cells does not affect mitochondrial mRNA stability, 16S rRNA processing or modification.…”
Section: Rna Helicasessupporting
confidence: 72%
“…This protein localizes to foci in the vicinity of nucleoids [129], the RNA granules [128]. Silencing of DHX30 in human cells produces a very severe translation defect that correlates with a profound loss of monosomes.…”
Section: Rna Helicasesmentioning
confidence: 99%
“…1). Among them are the cytoplasmic RNP assemblies -stress granules (SGs) (Kedersha et al, 1999), processing bodies (PBs) (Eulalio et al, 2007a;Jain and Parker, 2013), GW-bodies (Eulalio et al, 2009;Stoecklin and Kedersha, 2013), germline P-granules (also known as P-bodies) (Brangwynne et al, 2009;Voronina et al, 2011), neuronal granules (Buchan, 2014), mitochondrial RNA granules (MRGs) (Antonicka and Shoubridge, 2015), as well as the nuclear RNP assemblies -nucleoli (Brangwynne et al, 2011;Mahboubi and Stochaj, 2014), nuclear pores (Frey et al, 2006), Cajal bodies (CBs) (Machyna et al, 2013;Staneǩ and Neugebauer, 2006), promyelocytic leukemia (PML) bodies, histone locus body (Nizami et al, 2010), splicing speckles, other nuclear speckles and paraspeckles (Fox and Lamond, 2010;Knott et al, 2016;Shevtsov and Dundr, 2011).…”
Section: Introductionmentioning
confidence: 99%