Mitochondrial targeting fluorescent probe for specific detection of nickel ions and biological imaging

Three–Four Photon Transition Mn(II) Complex Monitoring Lysosome-Related ATP in Real Time via Fluorescence Lifetime Imaging

“…At present, the colorimetric method and fluorescent probe are commonly reported for detecting biomolecules. Compared with the colorimetric method, which has low sensitivity and is easily interfered, fluorescent probes stand out for their high sensitivity and excellent in situ imaging ability. − What is more is that it is notable that higher order multiphoton probes with near-infrared excitation show less photodamage and longer penetration depth. Because the excitation efficiency of higher order nonlinear behavior is limited, a high concentration of fluorescent dyes, which may cause molecular aggregation, leading to fluorescence quenching, is usually required to overcome this defect .…”