Mitochondrial β-oxidation of saturated fatty acids in humans

Adeva‐Andany, María M.; Carneiro-Freire, Natalia; Seco-Filgueira, Mónica; Fernández‐Fernández, Carlos; Mouriño-Bayolo, David

doi:10.1016/j.mito.2018.02.009

Cited by 246 publications

(166 citation statements)

References 106 publications

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“…The results from GO analysis indicated that C16:0 and C18:0 were involved in some of the same biological processes, including cellular response to glucose starvation, positive regulation of fatty acid oxidation, arachidonic acid metabolic process, thioester metabolic process, long term synaptic depression and urate metabolic process. Most of these biological processes have been widely explored in studies on fatty acids metabolism and function [32][33][34]. Further pathway analysis showed that the common pathways possibly regulated by C16:0 and C18:0 include cytokine-cytokine receptor intervention, prolactin signaling pathway, retinol metabolism, fatty acid degradation, peroxisome, steroid hormone biosynthesis, PPAR signaling pathway, and arachidonic acid metabolism.…”

Section: C18:0/c16:0 Ratio and Insulin Resistancementioning

confidence: 99%

A high fat diet with a high C18:0/C16:0 ratio induced worse metabolic and transcriptomic profiles in C57BL/6 mice

Wang

Song

et al. 2020

Preprint

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Abstract Background: Differential effects of individual saturated fatty acids (SFAs), particularly stearic acid (C18:0), relative to the shorter-chain SFAs have drawn interest for more accurate nutritional guidelines. However, specific biologic and pathologic functions that can be assigned to particular SFAs are very limited. The present study was designed to compare changes in metabolic and transcriptomic profiles in mice caused by a high C18:0 diet and high palmitic acid (C16:0) diet.Methods: Male C57BL/6 mice were assigned to a normal fat diet (NFD), a high fat diet with high C18:0/C16:0 ratio (HSF) or an isocaloric high fat diet with a low C18:0/C16:0 ratio (LSF) for 10 weeks. An oral glucose tolerance test, 72-h energy expenditure measurement and CT scan of body fat were done before sacrifice. Fasting glucose and lipids were determined by an autobiochemical analyzer. Blood insulin, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6) levels were measured by enzyme-linked immunosorbent assay methods. Free fatty acids (FFAs) profiles in blood and liver were determined by using gas chromatography-mass spectrometry. Microarray analysis was applied to investigate changes in transcriptomic profiles in the liver. Pathway analysis and gene ontology analysis were applied to describe the roles of differentially expressed mRNAs. Results: Compared with the NFD group, body weight, body fat ratio, fasting blood glucose, insulin, homeostasis model assessment of insulin resistance (HOMA-IR), triglyceride, IL-6, serum and liver FFAs including total FFAs, C16:0 and C18:0 were increased in both high fat diet groups and were much higher in the HSF group than those in the LSF group. Both HSF and LSF mice exhibited distinguishable long non-coding RNA (lncRNA), microRNA and mRNA expression profiles when compared with those of NFD mice. Additionally, more differentially expressed lncRNAs and mRNAs were observed in the HSF group than in the LSF group. Some biological functions and pathways, other than energy metabolism regulation, were identified as differentially expressed mRNAs between the HSF group and the LSF group. Conclusion: The high fat diet with a high C18:0/C16:0 ratio induced more severe glucose and lipid metabolic disorders and inflammation and affected expression of more lncRNAs and mRNAs than an isocaloric low C18:0/C16:0 ratio diet in mice. These results provide new insights into the differences in biological functions and related mechanisms, other than glucose and lipid metabolism, between C16:0 and C18:0.

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Section: C18:0/c16:0 Ratio and Insulin Resistancementioning

confidence: 99%

A high fat diet with a high C18:0/C16:0 ratio induced worse metabolic and transcriptomic profiles in C57BL/6 mice

Wang

Song

et al. 2020

Preprint

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“…It has been postulated that an increase in smoke exposure increases FA levels in cellular cytosol, resulting in protection against lipotoxicity. Conversely, impaired carnitine metabolism in COPD can cause accumulation of FA in the cytosol which can also lead to lipotoxicity . Metabolomics in COPD provides insight into the pathogenesis of the disease with alterations in sphingolipid metabolism and changes in lipids during increased oxidant stress.…”

Section: Metabolomic‐driven Discovery Of Biomarkersmentioning

confidence: 99%

Metabolomics in chronic lung diseases

et al. 2019

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Chronic lung diseases represent a significant global burden. Their increasing incidence and complexity render a comprehensive, multidisciplinary and personalized approach to each patient, critically important. Most recently, unique biochemical pathways and disease markers have been identified through large‐scale metabolomic studies. Metabolomics is the study of metabolic pathways and the measurement of unique biomolecules in a living system. Analysing samples from different compartments such as bronchoalveolar lavage fluid (BALF) and plasma has proven useful for the characterization of a number of pathological conditions and offers promise as a clinical tool. For example, several studies using mass spectrometry (MS) have shown alterations in the sphingolipid metabolism of chronic obstructive pulmonary disease (COPD) sufferers. In this article, we present a practical review of the application of metabolomics to the study of chronic lung diseases (CLD): COPD, idiopathic pulmonary fibrosis (IPF) and asthma. The insights, which the analytical strategies employed in metabolomics, have provided to the dissection of the biochemistry of CLD and future clinical biomarkers are explored.

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“…20 The thermal treatment, oxidized the fatty acid and lead to saturation of carbon chain with by lebrated H + ion. 24,25…”

Section: Physico-chemical Analysis Of Clarified Butter and Their Fracmentioning

confidence: 99%

Characterization of Thermal Fraction of Clarified Butter and its Applicability to Improve Bioavailability of Rosuvastatin

Suryawanshi¹,

Sakarkar²,

Kaur³

et al. 2019

IJPER

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Aim: The present investigation shows confirmatory evidence for the complex formation of Rosuvastatin with a fraction of Clarified Butter. Materials and Methods: Fractions of Clarified Butter prepared according to thermal behavior at different temperatures (30°C, 40°C, 50°C). Rosuvastatin biform complex with Clarified Butter in the ratio (1:1) and (1:2) w/w were prepared. Physicochemical properties of all fractions of Clarified Butter was determined as per Indian Pharmacopoeia. The fractions were subject to Fouriertransform Infrared spectroscopy (FTIR), Differential Scanning Calorimeters (DSC) and invitro release studies (Simulated gastric fluid, pH 1.2 for 2 h and simulated intestinal fluid, pH 6.8 for 6 h). Results: Based on these investigations; the optimized ratio (1:1) w/w were further characterized for DSC and FTIR studies. Where Rosuvastatin exhibited a strong carbonyl band at 1543.05 cm −1 , all fractions showed the disappearance of strong carbonyl band at 1543.05 cm −1 , spectra of biform complex shifted to higher frequency 1747.51 cm −1 (13.29 % increase), that confirmed carboxylic and hydroxyl group of both saturated and unsaturated fatty acid present in Clarified Butter. From in vitro release study the highest percent release i.e. 99.23±0.08% of Rosuvastatin was obtained from 1:1 biform complex. DSC thermogram revealed the formation of eutectic mixture by lowering the peak of pure Clarified Butter (67.4°C) to 58.10°C due to the formation of biform complex. Conclusion: Thus, Clarified Butter and its fraction illustrated enhanced bioavailability of Rosuvastatin.

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Mitochondrial β-oxidation of saturated fatty acids in humans

Cited by 246 publications

References 106 publications

A high fat diet with a high C18:0/C16:0 ratio induced worse metabolic and transcriptomic profiles in C57BL/6 mice

A high fat diet with a high C18:0/C16:0 ratio induced worse metabolic and transcriptomic profiles in C57BL/6 mice

Metabolomics in chronic lung diseases

Characterization of Thermal Fraction of Clarified Butter and its Applicability to Improve Bioavailability of Rosuvastatin

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