Mitodepressive and chromotoxic impact of nicotinamide on root meristems of Cyamopsis tetragonoloba L.

Abstract

The present study was undertaken to study the effect of nicotinamide at different concentrations viz.0.5%, 1.0%, 1.5%, 2.0% on root meristems of Cyamopsis tetragonoloba L. Mitotic study reveals various types of chromosomal aberrations like scattering(C-metaphase), precocious, unorientations, laggard, stickiness, bridges etc. Among these scattering was most prominent followed by precocious movement at metaphase and stickiness at anaphase. The AMI is expressed in terms of RDR while the chromosomal abnormalities in terms of RAR. The RDR considerably became more negative and RAR was increased along with increasing doses of nicotinamide. Increase in the negative value of RDR was directly proportional to the severity of the mitotic inhibition. Thus it was found that along with increasing treatment doses of nicotinamide the total abnormality percent increased and the active mitotic index decreased which shows potential toxicity of nicotinamide at their higher doses in this plant.

Nicotinamide and Azospirillum brasilense improves the quality of Coffea arabica seedlings

“…In contrast, the inhibitory effect observed at the highest nicotinamide doses for PH (Figure 2A), SD (Figure 2B), NL (Figure 2C), LA (Figure 2D), and SDM (Figure 3A) was due to high concentrations of nicotinamide, which block the mitotic cycle during the interphase, which can result in a long G2 * and ** -Significant at p ≤ 0.05 and ≤ 0.01 by the F-test, respectively period, inhibiting DNA synthesis, as verified by Kumar & Shikha (2012). These authors tested different concentrations of nicotinamide (0.5, 1.0, 1.5, and 2.0%), verifying that the active mitotic index (frequency of cell division) of the root meristem progressively decreased with increasing doses of nicotinamide and verified that the relative division rate was negative and directly proportional to the severity of mitotic inhibition.…”