Mitogenic capacity of oxidized human B cell lines

Ren, Ee Chee; Chan, Soh Ha

doi:10.1002/eji.1830141218

Cited by 2 publications

(1 citation statement)

References 13 publications

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“…For use as mitogenic feeders, allogeneic B cells were irradiated (10,000 rads) and incubated in serum-free RPMI with neuraminidase (0.04 U/10 million feeder cells) and galactose oxidase (0.1 U/10 million feeder cells) for 90 min at 37 C in 5% CO 2 (17)(18)(19)(20)(21). Tissue specimens were finely minced (without proteases) and incubated in 2-mL wells in T cell medium (RPMI 1640 containing glutamine, 10% heat-inactivated human serum, and penicillin/streptomycin).…”

Section: Establishment Of T Cell Lines (Tcl) and Clones (Tcc)mentioning

confidence: 99%

Relationship between Disease Duration and Predominant Orbital T Cell Subset in Graves’ Ophthalmopathy

Aniszewski

Valyasevi

Bahn

2000

The Journal of Clinical Endocrinology & Metabolism

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We sought to determine whether the predominant orbital T helper (T(H)) cell subset in orbital T cell clones established from patients with Graves' ophthalmopathy (GO) might be related to disease duration. A total of 117 clones were established from orbital adipose/connective tissues of 6 GO patients, and cytokine production was measured in 57 CD3+CD4+ clones. T(H)1-type clones were predominant in cultures from patients with recent onset (<2 yr) Graves' hyperthyroidism (n = 44; TH1/TH0/TH2 = 57/29/14%) or GO (n = 53 clones; TH1/TH0/TH2 = 47/30/23%). In contrast, TH2-type clones predominated in cultures from patients with more remote onset (>2 yr) hyperthyroidism (n = 13; TH1/TH0/TH2 = 0/31/69%; P < 0.005) or GO (n = 4; TH1/TH0/TH2 = 0/25/75%; P = 0.05). In addition, we established T cell clones from 1 TH1-dominant patient with recent-onset thyroid and eye disease using either IL-2 (12.5 ng/mL) alone or IL-2 plus IL-4 (5 ng/mL) and found no shift toward recovery of TH2-type clones in the latter. In conclusion, although the CD3+CD4+ clones characterized were not necessarily tissue antigen specific, our findings suggest that cell-mediated (TH1-type) immune reactions may predominate in the orbit in early GO, whereas humoral immunity (TH2-type) might play the greater role in later stages of the disease.

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Section: Establishment Of T Cell Lines (Tcl) and Clones (Tcc)mentioning

confidence: 99%

Relationship between Disease Duration and Predominant Orbital T Cell Subset in Graves’ Ophthalmopathy

Aniszewski

Valyasevi

Bahn

2000

The Journal of Clinical Endocrinology & Metabolism

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Prostaglandin E2 Selectively Inhibits Human CD4+ T Cells Secreting Low Amounts of Both IL-2 and IL-4

Stuart

1999

The Journal of Immunology

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PGE2 is a potent inflammatory mediator with profound immune regulatory actions. The present study examined the effects of PGE2 on the activation/proliferation of CD4+ T cells using 37 cloned CD4+ T cell lines. Ten T cell clones sensitive to PGE2 and 10 T cell clones resistant to PGE2, as measured by proliferation in response to anti-CD3 Ab, were selected for comparison. It was found that the PGE2-sensitive T cells were characterized by low production (<200 pg/ml) of both IL-2 and IL-4, while PGE2-resistant T cells secreted high levels (>1000 pg/ml) of IL-2, IL-4, or both. The roles of IL-2 and IL-4 were confirmed by the finding that addition of exogenous lymphokines could restore PGE2-inhibited proliferation, and PGE2-resistant Th1-, Th2-, and Th0-like clones became PGE2 sensitive when IL-2, IL-4, or both were removed using Abs specific for the respective lymphokines. In addition, we showed that the CD45RA expression in PGE2-sensitive T cells was significantly lower than that in PGE2-resistant cells (mean intensity, 1.2 ± 0.6 vs 7.8 ± 5.7; p = 0.001). In contrast, CD45RO expression in PGE2-sensitive T cells was significantly higher that that in PGE2-resistant cells (mean intensity, 55.7 ± 15.1 vs 33.4 ± 12.9; p = 0.02). In summary, PGE2 predominantly suppressed CD45RA−RO+ CD4+ T cells with low secretion of both IL-2 and IL-4.

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Mitogenic capacity of oxidized human B cell lines

Cited by 2 publications

References 13 publications

Relationship between Disease Duration and Predominant Orbital T Cell Subset in Graves’ Ophthalmopathy

Relationship between Disease Duration and Predominant Orbital T Cell Subset in Graves’ Ophthalmopathy

Prostaglandin E2 Selectively Inhibits Human CD4+ T Cells Secreting Low Amounts of Both IL-2 and IL-4

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