Mitogenic Properties of Aromatic Retinoids: in vivo and in vitro Effects on Epidermal Cells

Pohl, J.; Christophers, Enno

doi:10.1007/978-3-642-68023-6_17

Cited by 10 publications

(3 citation statements)

References 8 publications

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“…Vitamin A controls epithelial differentiation [3 -11] and proliferation [12][13][14][15] in a number of in vitro model systems. Retinoic acid has been shown to stimulate adult guinea pig keratinocyte proliferation during the first 24-60 h of culture [12][13][14], possibly by altering the plating efficiency and cell surface properties of the freshly trypsinized cells.…”

Section: Introductionmentioning

confidence: 99%

“…After this initial effect, retinoic acid appears to affect the differentiation of the keratinocytes, primarily by influencing the attachement/ detachment ratio of the keratinocytes [9][10][11] and by decreasing keratin [7] and cell envelope formation [8]. Retinoids (vitamin A analogs) other than retinoic acid, however, appear to have different effects on in vitro keratinocytes [15].…”

Section: Introductionmentioning

confidence: 99%

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Regulation of the expression of epidermal keratinocyte proliferation and differentiation by vitamin A analogs

Marcelo

Madison

1984

Arch Dermatol Res

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A number of vitamin A analogs (retinoids) were used to manipulate the growth of epidermal keratinocytes in culture. The retinoids used were the TMMP analog of ethyl retinoate (Ro 10-9359), 13-cis retinoic acid, all trans retinoic acid and retinol (trans). These were added to primarily neonatal mouse epidermal keratinocyte cultures that proliferate, stratify, and differentiate over 2-3 weeks. [3H]Tdr labeling technics were used to quantitate proliferation. A histologic stain, and a four buffer protein extraction protocol, used in conjunction with polyacrylamide gel electrophoresis and fluorographic technics, were used to assess the differentiation of the cultures. Our results showed that all of the vitamin A analogs we tested inhibited keratinocyte proliferation. Quantitation of specific differentiation proteins showed that Ro 10-9359 and 13-cis retinoic acid partially inhibited the differentiation of the cultures. The Ro 10-9359 retinoid was unusual in that it increased the synthesis of keratohyalin granule-related proteins. These studies showed that inhibition of basal cell proliferation did not result in the obligatory expression of cell differentiation and that at least one of the events that is a part of epidermal keratinocyte differentiation can be separately controlled.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Regulation of the expression of epidermal keratinocyte proliferation and differentiation by vitamin A analogs

Marcelo

Madison

1984

Arch Dermatol Res

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“…as the velocity of the drug-induced epithe!ial restitution, as recently shown in epidermal lesions of 1.p. ( 10 ) . Moreover, the small number of biopsies so far available does not allow definite conclusions to be drawn with regard to the basic cellular mechanisms involved in the benefit of aromatic retinoid therapy in oral l.p.…”

Section: Resultsmentioning

confidence: 99%

Epithelial cell proliferation of oral lichen planus in patients treated with an aromatic retinoid

Schell,

Hornstein,

Deinlein

et al. 1983

Acta Derm Venereol

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In 4 patients with lichen planus mucosae (l.p.m.) biopsies were taken from the involved and uninvolved buccal sides before treatment and from the affected side after a 3-week systemic therapy with aromatic retinoid (Ro 10-9359; 50 mg/die). After in vitro labelling with 3H-thymidine the epithelial cell proliferation was determined histo-autoradiographically. In atrophic lesions of l.p.m., increased 3H-thymidine labelling indices were observed during retinoid therapy. After 3 weeks of treatment the augmented relative portion of labelled basal cells (quotient LIbas/LIsuprabas) was found to be lowered, indicating a tendency of proliferating cells to regain normal distribution in the progenitor compartment of the mucosa epithelium.

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Econazol

Bruchhausen

Ebel

Hackenthal

et al. 1993

Hagers Handbuch Der Pharmazeutischen Praxis

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Mitogenic Properties of Aromatic Retinoids: in vivo and in vitro Effects on Epidermal Cells

Cited by 10 publications

References 8 publications

Regulation of the expression of epidermal keratinocyte proliferation and differentiation by vitamin A analogs

Regulation of the expression of epidermal keratinocyte proliferation and differentiation by vitamin A analogs

Epithelial cell proliferation of oral lichen planus in patients treated with an aromatic retinoid

Econazol

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