Mitogn induced proliferation of isolated adult mouse schwann cells

Abstract

The proliferation of neonatal Schwann cells (SCs) in response to mitogenic agents has been well analyzed in vitro, but a limited range of mitogens have been defined. We investigated whether three identified neonatal SC mitogens [glial growth factor (GGF), platelet-derived growth factor BB (PDGF-BB), and basic fibroblast growth factor (bFGF)] are required to stimulate mitosis of adult SCs. Adult SCs were isolated from mouse sciatic nerves by mechanical and chemical dissociation, following three experimental steps: 1) culturing the dissociated cells for 24 hr in 10% FCS-F12 medium, 2) culturing these cells in serum-free medium for the next 48 hr, and 3) purifying adult SCs by differential adhesion. We describe a new method for preparation of SCs from peripheral nerves of adult mouse that provides 99.5% pure SCs populations at cell yields of greater than 3 x 10(3) cells/mg of starting nerve wet weight within 5 culture days. Although mitosis of SCs in culture in response to mitogens requires the presence of serum, the complex nature of serum renders difficult a complete analysis of mitogens required for SCs DNA synthesis, so we examined the proliferating response of adult SCs to GGF, PDGF-BB, and bFGF in serum-free medium. GGF alone had mitogenicity for adult SCs in a dose-dependent manner, and synergistic activation coupling with forskolin was not observed. Neither PDGF-BB nor bFGF was mitogenic for adult SCs when used alone or with forskolin.(ABSTRACT TRUNCATED AT 250 WORDS)

Chronically denervated rat schwann cells respond to GGF in vitro

“…Previous studies have shown that GGF2 influences both the motility and proliferation of Schwann cells in vitro (Lemke and Brockes, 1984;Mahanthappa et al, 1996;Zhang et al, 1995;Rutkowski et al, 1995). We have confirmed these effects on acutely denervated Schwann cells, using a concentration of GGF2, which stimulates both.…”

Olfactory ensheathing glia and Schwann cells: two of a kind?

“…forskolin. Schwann cell proliferation is also enhanced by several mitogens, especially heregulin (HRG) proteins, added to the culture medium (Levi et al 1995;Zhang et al 1995). The effects of these factors are potentiated by serum or forskolin .…”

Influence of IN-1 antibody and acidic FGF-fibrin glue on the response of injured corticospinal tract axons to human Schwann cell grafts

“…It is also possible that aFGF influences the interaction between injured, sprouting CST fibers and the grafted SCs. Basic FGF may be capable of inducing SC mitogenesis in the presence of other supportive molecules (Peulve et al, 1994;Tzeng et al, 1995;B.T. Zhang et al, 1995).…”