Mitomycin-Induced Lysis of Clostridium Sordellii

PLATE XVIIT is still technically difficult to distinguish some strains of Clostridium oedematiens (C. novyi) of types B and D (Rutter and Collee, 1969). Type-A strains of C. oedematiens produce a haemolytic lecithinase (the $oxin) which is distinct from the haemolytic lecithinase (@-toxin) produced by type-B and type-D strains. As type-D strains are known to produce more of the /?-toxin lecithinase than type-B strains, it was hoped to determine ranges of this lecithinase activity, here referred to as p-lecithinase, that would discriminate between the two types. In the course of studies on the lysogeny of clostridia (Kiritani et al., 1973; Shimamura et al., 1974), we found that type-D strains differed from type-B strains of C. oedematiens in their susceptibility t Q mitomycin C (MC). This was investigated further in the present study with a view to differentiating type-B and type-D strains. Since treatment of bacteria in the lysogenic state by MC induces multiplication of phage, and since lysogeny is sometimes related to bacterial toxigenicity, we also examined strains of C. oedematiens of types B and D in parallel for susceptibility to MC and for production of the /?-lecithinase. MATERIALS AND METHODSBacterial strains C. oedematiens type A . Toxigenic strains 77101, 77103, 37101, 37106, and 37110 and non-toxigenic strains 3-1 103,3-1105,3-1111, and 3-1 112 are stock strains isolated from soil by one of the authors in 1963 and typed by Nishida and Nakagawara (1964).

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“…Preparation of samples and the procedures for electronmicroscopy were as described by Shimamura et al (1974).…”

Section: Detection Of Phage Particles By Electronmicroscopymentioning

confidence: 99%

Susceptibility to Mitomycin C and Lecithinase Activities of Clostridium Oedematiens (C. Novyi) Types B and D

Nakamura

Takematsu

Nishida

1975

Journal of Medical Microbiology

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Bacteriophages of the Genus Clostridium

Ogata

Hongo

1979

Advances in Applied Microbiology

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Clostridium sordellii bacteriophage active on Clostridium difficile

Schallehn¹

1985

Lett Appl Microbiol

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Among five strains of Clostridium difficile and 39 strains of Cl. sordellii tested, one Cl. difficile phage and four Cl. sordellii phages were found to be lytic for Cl. difficille strain 2. The five phages were similar in morphology, showing a polyhedral head of 60 nm in diameter, a tail of 105–120 nm, a contractile tail sheath and a base plate. They were sensitive to heat (60°C/10 min) and stable at 4°C for at least 6 months. As the phage donor strains and the indicator strain were not cytotoxigenic, no phage‐infected culture of Cl. difficile 2 was able to produce cytotoxin.

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Mitomycin-Induced Lysis of Clostridium Sordellii

Cited by 4 publications

References 7 publications

Susceptibility to Mitomycin C and Lecithinase Activities of Clostridium Oedematiens (C. Novyi) Types B and D

Susceptibility to Mitomycin C and Lecithinase Activities of Clostridium Oedematiens (C. Novyi) Types B and D

Bacteriophages of the Genus Clostridium

Clostridium sordellii bacteriophage active on Clostridium difficile

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