Mitophagy is primarily due to alternative autophagy and requires the MAPK1 and MAPK14 signaling pathways

Abstract

Abbreviations: 3-MA, 3-methyladenine; ATG, autophagy-related; CCCP, carbonyl cyanide m-chlorophenyl hydrazone; ER, endoplasmic reticulum; FUNDC1, FUN14 Domain Containing 1; LC3, microtubule-associated protein 1 light chain 3; MAPK, mitogen-activated protein kinase; MEF, mouse embryonic fibroblast; MEK, MAPK-ERK kinase; MKK, MAP kinase kinase; PE, phosphatidylethanolamine; PINK1, PTEN-induced putative kinase protein 1; PTEN, phosphatase and tensin homolog; siRNA, short interfering RNA; SQSTM1, sequestosome 1; Tet, tetracycline.In cultured cells, not many mitochondria are degraded by mitophagy induced by physiological cellular stress. We observed mitophagy in HeLa cells using a method that relies on the pH-sensitive fluorescent protein Keima. With this approach, we found that mitophagy was barely induced by carbonyl cyanide m-chlorophenyl hydrazone treatment, which is widely used as an inducer of PARK2/Parkin-related mitophagy, whereas a small but modest amount of mitochondria were degraded by mitophagy under conditions of starvation or hypoxia. Mitophagy induced by starvation or hypoxia was marginally suppressed by knockdown of ATG7 and ATG12, or MAP1LC3B, which are essential for conventional macroautophagy. In addition, mitophagy was efficiently induced in Atg5 knockout mouse embryonic fibroblasts. However, knockdown of RAB9A and RAB9B, which are essential for alternative autophagy, but not conventional macroautophagy, severely suppressed mitophagy. Finally, we found that the MAPKs MAPK1/ERK2 and MAPK14/p38 were required for mitophagy. Based on these findings, we conclude that mitophagy in mammalian cells predominantly occurs through an alternative autophagy pathway, requiring the MAPK1 and MAPK14 signaling pathways.

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“…Although the mechanism remains to be elucidated, one possibility is that Atg5-and Atg7-independent alternative autophagy could compensate for the clearance of the damaged mitochondria as indicated by a recent paper. 43 Another possibility is that mitochondrial biogenesis could be suppressed with aging.…”

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