MitoQ supplementation prevent long-term impact of maternal smoking on renal development, oxidative stress and mitochondrial density in male mice offspring

Abstract

To investigate the effect of maternal MitoQ treatment on renal disorders caused by maternal cigarette smoke exposure (SE). We have demonstrated that maternal SE during pregnancy increases the risk of developing chronic kidney disease (CKD) in adult offspring. Mitochondrial oxidative damage contributes to the adverse effects of maternal smoking on renal disorders. MitoQ is a mitochondria-targeted antioxidant that has been shown to protect against oxidative damage-related pathologies in many diseases. Female Balb/c mice (8 weeks) were divided into Sham (exposed to air), SE (exposed to cigarette smoke) and SEMQ (exposed to cigarette smoke with MitoQ supplemented from mating) groups. Kidneys from the mothers were collected when the pups weaned and those from the offspring were collected at 13 weeks. Maternal MitoQ supplementation during gestation and lactation significantly reversed the adverse impact of maternal SE on offspring’s body weight, kidney mass and renal pathology. MitoQ administration also significantly reversed the impact of SE on the renal cellular mitochondrial density and renal total reactive oxygen species in both the mothers and their offspring in adulthood. Our results suggested that MitoQ supplementation can mitigate the adverse impact of maternal SE on offspring’s renal pathology, renal oxidative stress and mitochondrial density in mice offspring.

Why Do Intrauterine Exposure to Air Pollution and Cigarette Smoke Increase the Risk of Asthma?

“…Our previous studies in mice have repeatedly shown that MSE can reduce the level of endogenous antioxidant Manganese Superoxide Dismutase in the brain, kidney, and lungs of adult offspring accompanied by increased Reactive Oxygen Species (ROS) levels in those organs; interestingly, antioxidant supplementation during pregnancy could completely or partially reverse the adverse effects on those organs induced by MSE (Chan et al, 2017;Sukjamnong et al, 2017Sukjamnong et al, , 2018. The endogenous antioxidant enzyme system is established in the second and third trimester of pregnancy and continues to develop in early childhood (Fanucchi, 2004).…”

E‐cigarettes damage the liver and alter nutrient metabolism in pregnant mice and their offspring

“…The content of mtDNA was measured by quantifying mRNA expression of the mitochondrial-encoded gene cytochrome c oxidase subunit 1 (COX1; forward primers 5 -ACTATACTACTACTAA-CAGACCG-3 , reverse primers 5 -GGTTC TTTTTTTCCGGAGTA-3 ) against the nuclearencoded gene cyclophilin A (Ppia) (forward primers 5 -ACACGCCATAATGGCACTGG-3 , reverse primers 5 -CAGTCTTGGCAGTGCAGAT-3 ), as we previously published. 14,15 COX1 expression was standardized to Ppia using the Ct method. The average of the sham group was assigned the calibrator against which all other results were expressed as fold changes.…”

Non-invasive real-time imaging of reactive oxygen species (ROS) using multispectral auto-fluorescence imaging technique: a novel tool for redox biology

“…In this study we investigated neoplastic cells (HeLa and PANC1), human bone marrow derived mesenchymal stem cells (MSC) (isolated as described previously [21,22]) and cryopreserved whole kidney tissue sections obtained from 13 week old Balb/c mice, which were exposed to either air or smoke in utero, as we have previously demonstrated that they express increased levels of ROS [23]. Full details of cell culture and ethics approvals are included in Supplementary Material note 1.…”