Mitosin/CENP-F in mitosis, transcriptional control, and differentiation

Ma, Li; Zhao, Xiangshan; Zhu, Xueliang

doi:10.1007/s11373-005-9057-3

Cited by 59 publications

(54 citation statements)

References 84 publications

(153 reference statements)

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“…However, little is known about the relevance to rhabdomyosarcomagenesis of the candidate genes we identified located within these genomic regions. Among the genes found overexpressed, CENPF encodes a multifunctional protein which is involved in mitotic control and muscle cell differentiation (Ma et al, 2006). It has been found amplified and overexpressed in other tumor types (Pimkhaokham et al, 2000;van den Boom et al, 2003;Grutzmann et al, 2004) and is associated with Wilms tumor progression (Zirn et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Genomic imbalances in rhabdomyosarcoma cell lines affect expression of genes frequently altered in primary tumors: An approach to identify candidate genes involved in tumor development

Missiaglia

Selfe

Hamdi

et al. 2009

Genes Chromosomes & Cancer

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Rhabdomyosarcomas (RMS) are the most common pediatric soft tissue sarcomas. They resemble developing skeletal muscle and are histologically divided into two main subtypes; alveolar and embryonal RMS. Characteristic genomic aberrations, including the PAX3- and PAX7-FOXO1 fusion genes in alveolar cases, have led to increased understanding of their molecular biology. Here, we determined the effect of genomic copy number on gene expression levels through array comparative genomic hybridization (CGH) analysis of 13 RMS cell lines, confirmed by multiplex ligation-dependent probe amplification copy number analyses, combined with their corresponding expression profiles. Genes altered at the transcriptional level by genomic imbalances were identified and the effect on expression was proportional to the level of genomic imbalance. Extrapolating to a public expression profiling dataset for 132 primary RMS identified features common to the cell lines and primary samples and associations with subtypes and fusion gene status. Genes identified such as CDK4 and MYCN are known to be amplified, overexpressed, and involved in RMS tumorigenesis. Of the many genes identified, those with likely functional relevance included CENPF, DTL, MYC, EYA2, and FGFR1. Copy number and expression of FGFR1 was validated in additional primary material and found amplified in 6 out of 196 cases and overexpressed relative to skeletal muscle and myoblasts, with significantly higher expression levels in the embryonal compared with alveolar subtypes. This illustrates the ability to identify genes of potential significance in tumor development through combining genomic and transcriptomic profiles from representative cell lines with publicly available expression profiling data from primary tumors.

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Section: Discussionmentioning

confidence: 99%

Genomic imbalances in rhabdomyosarcoma cell lines affect expression of genes frequently altered in primary tumors: An approach to identify candidate genes involved in tumor development

Missiaglia

Selfe

Hamdi

et al. 2009

Genes Chromosomes & Cancer

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“…Moreover, the results of different studies are not homogeneous as to the cell fate after CENPF silencing (Holt et al 2005, Laoukili et al 2005, Feng et al 2006. Ma et al (2006) suggest that this may be caused by different efficiencies of the mRNA silencing. We, however, agree with the hypothesis of Feng et al (2006), who assumed that the variation in results might be caused by the usage of different cell lines.…”

Section: Discussionmentioning

confidence: 99%

Silencing CENPF in bovine preimplantation embryo induces arrest at 8-cell stage

Toralova¹,

Šušor²,

Němcová³

et al. 2009

Reproduction

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Identification of genes that are important for normal preimplantation development is essential for understanding the basics of early mammalian embryogenesis. In our previous study, we have shown that CENPF (mitosin) is differentially expressed during preimplantation development of bovine embryos. CENPF is a centromere-kinetochore complex protein that plays a crucial role in the cell division of somatic cells. To our best knowledge, no study has yet been done on either bovine model, or oocytes and preimplantation embryos. In this study, we focused on the fate of bovine embryos after injection of CENPF double-stranded RNA (dsRNA) into the zygotes. An average decrease of CENPF mRNA abundance by 94.9% or more and an extensive decline in immunofluorescence staining intensity was detected relative to controls. There was no disparity between individual groups in the developmental competence before the 8-cell stage. However, the developmental competence rapidly decreased then and only 28.1% of CENPF dsRNA injected 8-cell embryos were able to develop further (uninjected control: 71.8%; green fluorescent protein dsRNA injected control: 72.0%). In conclusion, these results show that depletion of CENPF mRNA in preimplantation bovine embryos leads to dramatic decrease of developmental competence after embryonic genome activation.

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“…Although CENPF is initially known for its association with the centromere-kinetochore complex and chromosomal segregation, previous studies have demonstrated that CENPF may play a role in the regulation of the mitosis and proliferation of malignant cell growth, which are mediated by protein interactions (13,(29)(30)(31)(32). Thus, the actual functional role of CENPF is more elusive and complex.…”

Section: Irs Of Cenpf In Our Cohortmentioning

confidence: 99%