Mitotic cycles in human cell strains with sex chromosomes aneuploidy

Kukharenko, V. I.; Grinberg, K. N.; Kuliev, Anver

doi:10.1007/bf00283635

Cited by 17 publications

(10 citation statements)

References 10 publications

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“…Thus it would seem that the cell generation time of the 45,X cells becomes gradually longer than that of normal 46,XX cells. This finding is in agreement with the results of Cure et al (1974) and Simpson and Lebeau (1981) but contradicts those of Barlow (1972) and Kukharenko et al (1978). The findings of Hoehn et al (1980), although interpreted as negative by the authors, also indicated a longer cell cycle in 45,X cells.…”

Section: Resultssupporting

confidence: 59%

“…Cure, Bou6 and Bou6 (1974) and Simpson and Lebeau (1981) reported a prolonged cell cycle in cells with a deleted or missing X chromosome. Kukharenko, Grinberg and Kuliev (1978), on the other hand, found a shortened cell generation time in several aneuploid cell lines but no association with the type of aneuploidy. The results of other studies seem to indicate that the number of sex chromosomes has no effect on cell cycle parameters (Kukharenko, Kuliev, Grinberg and Terskikh 1974, Yurov 1978, Hoehn, Simpson, Bryant, Rabinovitch, Salk and Martin 1980 We tested Barlow's (1973) hypothesis by studying growth of 45,X and 47,XYY cells in cell culture.…”

Section: Introductionmentioning

confidence: 90%

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Effect of sex chromosome aneuploidy on growth of human skin fibroblasts in cell culture

Varrela¹,

Larjava²,

Järveläinen³

et al. 1989

Annals of Human Biology

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Growth of human skin fibroblast lines with sex chromosome aneuploidy was studied in cell culture. Two lines were derived from individuals with 45,X and two from individuals with 47,XYY chromosome constitution. The aneuploid cell lines were compared with normal skin fibroblasts derived from age-matched same-sex siblings. To ensure similar conditions in culture, each study-control pair was treated simultaneously and equally in all steps. Growth in both 45,X cell cultures slowed down earlier than in control cultures. One of the 47,XYY cultures grew faster and the other at a similar speed to the control culture. Thus, the 45,X cell lines seemed to have lesser, and one of the 47,XYY cell lines greater, growth potential than the 46,XX and 46,XY cells, respectively. These differences in the final cell numbers may relate to changes in the rate of cell division of the aneuploid cell lines. However, the results do not support the hypothesis that an increasing number of sex chromosomes decreases the rate of cell division.

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Section: Resultssupporting

confidence: 59%

Section: Introductionmentioning

confidence: 90%

Effect of sex chromosome aneuploidy on growth of human skin fibroblasts in cell culture

Varrela¹,

Larjava²,

Järveläinen³

et al. 1989

Annals of Human Biology

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“…The cytogenetic findings of this study corroborate the hypothesis of an apparent 45,X chromosomal complement as a consequence of a selective loss of a second cell line during embryonic development. Data on the effect of aneuploidy of sex chromosomes on the mitotic cycle, lifespan, and proliferating activity of cultivated fibroblasts are contradictory (Polani 1970;Barlow 1972;Kukharenko et al 1978;Hoehn et al 1980;Verp et al 1988). From our data, either a shortened lifespan of cells containing marker chromosomes or a selective loss of marker chromosomes is the most likely explanation for the observed decrease in the percentage of cells displaying marker chromosomes after serial subcultivation.…”

Section: Discussioncontrasting

confidence: 50%

Mosaicism in 45,X Turner syndrome: does survival in early pregnancy depend on the presence of two sex chromosomes?

et al. 1992

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Cytogenetic and molecular genetic findings in 91 patients with Turner syndrome are reported. In 87 patients, chromosome studies were carried out both in lymphocyte and fibroblast cultures. Mosaicism was demonstrated in 58 of these patients (66.7%), whereas only 18 (20.7%) were apparent non-mosaic 45,X, and 11 patients (12.6%) showed non-mosaic structural aberrations of the X chromosome. Among the mosaic cases 16 (18.4% of all patients) displayed a second cell line containing small marker chromosomes. The association of Y-specific chromosomal material with the presence of marker chromosomes was demonstrated in 6 out of 7 mixoploid fibroblast cell lines by polymerase chain reaction amplification and by Southern-blot analysis. The observation of ring formation and morphological variability in vivo and in vitro, and the continuous reduction in the percentage of cells containing marker chromosomes in longterm cultivation experiments indicated an increased instability of marker chromosomes. The findings suggest that in vivo selection of structurally altered sex chromosomes exists. Thus, the observation of apparent non-mosaic 45,X chromosomal complements in liveborn individuals with Turner syndrome does not contradict the hypothesis that some degree of mosaicism is necessary for survival in early pregnancy.

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“…Since then, contradictory reports have been published [Cure et al, 1974;Kukharenko et al, 1978;Hoehn et al,1980]. But a more recent investigation made by Simpson and Lebeau [ 19811 showed that cell generation time was prolonged in each of four 45, X cell lines, compared to that in 46,XX controls as well as in 46, X, del (X)(q13),46,X,del(X)(q22), and 46,X, del (X)(pll) lines.…”

Section: Aneuploidymentioning

confidence: 98%

Introduction to the study of pre‐and postnatal growth in humans: A review

Mendez

Opitz²,

Reynolds³

1985

Am. J. Med. Genet.

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This review is divided in several items. A brief introduction on the characterization of the growth processes is made; the ways of assessing fetal development and well-being, the factors acting on fetal growth and birth weight, the causes and post-natal consequences of prematurity and intrauterine growth retardation are discussed in the first part. The following items deal mainly with: the normal pattern of growth from birth to puberty according to sex, race, and nutritional status, with special mention to pubertal changes; methods for predicting adult height from skeletal age; the effect of hormones during pre- and post-natal life; and the genetics of adult stature. The remainder of this review deals with genetic causes of growth abnormalities. Constitutional delay of growth, familial short stature, hypothalamic-pituitary dwarfism, skeletal dysplasias and many genetic syndromes presenting intrauterine growth retardation are listed. Aneuploidy effects on human growth are extensively reviewed, and usual growth patterns in Down and Ullrich-Turner syndrome patients as well as other sex aneuploid individuals and mosaics are fully described. The influences of X and Y chromosomes on growth and maturation are also discussed. Finally, some remarks are made about overgrowth syndromes.

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Mitotic cycles in human cell strains with sex chromosomes aneuploidy

Abstract: A persistence of the embryonic type of mitotic cycle was found in postnatal strains with aneuploidy of sex chromosomes (45,X; 47,XXX; 49,XXXXX; 47,XYY; 49,XXXXY). Life-span and proliferating activity of the strains did not differ from those of diploid postnatal cells.

Cited by 17 publications

References 10 publications

Effect of sex chromosome aneuploidy on growth of human skin fibroblasts in cell culture

Effect of sex chromosome aneuploidy on growth of human skin fibroblasts in cell culture

Mosaicism in 45,X Turner syndrome: does survival in early pregnancy depend on the presence of two sex chromosomes?

Introduction to the study of pre‐and postnatal growth in humans: A review

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