Mitotic Exit and Separation of Mother and Daughter Cells

Weiss, Eric L.

doi:10.1534/genetics.112.145516

Cited by 131 publications

(173 citation statements)

References 394 publications

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“…The release becomes definitive following activation of the MEN, and promotes mitotic exit. If the MEN is not activated, CDC14p release is only transient (reviewed by Weiss, 2012). This has some similarity with the unstable SPB association of SIN proteins, particularly GFP-Sid1p.…”

Section: Spatial Instability Of Sin Proteins In the Early Statementioning

confidence: 98%

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Analysis ofS. pombeSIN protein SPB-association reveals two genetically separable states of the SIN

Wachowicz

Chasapi

Krapp

et al. 2014

Journal of Cell Science

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The Schizosaccharomyces pombe septation initiation network (SIN) regulates cytokinesis, and asymmetric association of SIN proteins with the mitotic spindle pole bodies (SPBs) is important for its regulation. Here, we have used semi-automated image analysis to study SIN proteins in large numbers of wild-type and mutant cells. Our principal conclusions are: first, that the association of Cdc7p with the SPBs in early mitosis is frequently asymmetric, with a bias in favour of the new SPB; second, that the early association of Cdc7p-GFP to the SPB depends on Plo1p but not Spg1p, and is unaffected by mutations that influence its asymmetry in anaphase; third, that Cdc7p asymmetry in anaphase B is delayed by Pom1p and by activation of the spindle assembly checkpoint, and is promoted by Rad24p; and fourth, that the length of the spindle, expressed as a fraction of the length of the cell, at which Cdc7p becomes asymmetric is similar in cells dividing at different sizes. These data reveal that multiple regulatory mechanisms control the SIN in mitosis and lead us to propose a two-state model to describe the SIN.

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Section: Spatial Instability Of Sin Proteins In the Early Statementioning

confidence: 98%

“…Although the SIN and MEN differ in their 'wiring' (reviewed by Simanis, 2003;Weiss, 2012), this points to a conserved role for Spg1p/TEM1p and Plo1p/CDC5p in regulating the SPB association of Cdc7p family kinases.…”

Section: What Regulates the Early-late Transition?mentioning

confidence: 99%

Analysis ofS. pombeSIN protein SPB-association reveals two genetically separable states of the SIN

Wachowicz

Chasapi

Krapp

et al. 2014

Journal of Cell Science

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“…Although the regulatory wiring of the MEN and SIN differ slightly in vegetative cells, previous studies have shown that the regulation of the MEN also differs significantly between meiosis and mitosis. During vegetative growth, MEN signalling originates from the SPB and involves a cascade of protein kinases, the activities of which are modulated by a GTPase (reviewed by Simanis, 2003;Weiss, 2012). By contrast, during meiosis, MEN signalling does not require either SPB association or the scaffold protein used in vegetative growth.…”

Section: Discussionmentioning

confidence: 99%

Dma1-dependent degradation of Septation Initiation Network proteins during meiosis inSchizosaccharomyces pombe

Krapp

2014

Journal of Cell Science

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The Schizosaccharomyces pombe septation initiation network (SIN) is required for cytokinesis during vegetative growth and for spore formation during meiosis. Regulation of the SIN during mitosis has been studied extensively, but less is known about its meiotic regulation. Here, we show that several aspects of SIN regulation differ between mitosis and meiosis. First, the presence of GTPbound Spg1p is not the main determinant of the timing of Cdc7p and Sid1p association with the spindle pole body (SPB) during meiosis. Second, the localisation dependencies of SIN proteins differ from those in mitotic cells, suggesting a modified functional organisation of the SIN during meiosis. Third, there is stage-specific degradation of SIN components in meiosis; Byr4p is degraded after meiosis I, whereas the degradation of Cdc7p, Cdc11p and Sid4p occurs after the second meiotic division and depends upon the ubiquitin ligase Dma1p. Finally, Dma1p-dependent degradation is not restricted to the SIN, as we show that Dma1p is needed for the degradation of Mcp6p (also known as Hrs1p) during meiosis I. Taken together, these data suggest that stage-specific targeted proteolysis plays an important role in regulating meiotic progression.

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“…In addition, they found that, like the S. cerevisiae ortholog, it plays an important role in mitosis. In S. cerevisiae, Cbk1 plays functions in the mitotic exit network (MEN) and in the regulation of cytokinesis (Weiss 2012). In addition, C. albicans cells lacking components of the RAM pathway show increased levels of multinucleate cells (Song et al 2008).…”

Section: Mitotic Defects Of Mps1 Mutants Are Exacerbated By Loss Of Cmentioning

confidence: 99%

“…Although it has been clearly linked to cell cycle and to daughter cell-specific gene expression (Saputo et al 2012;Weiss 2012), little is known regarding the potential function of the RAM pathway within other pathways that regulate cell morphogenesis and polarity. Our screens identified two genes that interact with CBK1 and are related, or are potentially related, to Cdc42 function.…”

Section: Cbk1 Interacts With Two Genes Related To Cell Polarity Throumentioning

confidence: 99%

Complex Haploinsufficiency-Based Genetic Analysis of the NDR/Lats Kinase Cbk1 Provides Insight into Its Multiple Functions in Candida albicans

et al. 2016

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Although the analysis of genetic interactions and networks is a powerful approach to understanding biology, it has not been applied widely to the pathogenic yeast Candida albicans. Here, we describe the use of both screening and directed genetic interaction studies based on complex haploinsufficiency to probe the function of the Regulation of Ace2 and Morphogenesis (RAM) pathway in C. albicans. A library of 5200 Tn7-mutagenized derivatives of a parental strain heterozygous at CBK1, the key kinase in the RAM pathway, was screened for alterations in serum-induced filamentation. Following confirmation of phenotypes and identification of insertion sites by sequencing, a set of 36 unique double heterozygous strains showing complex haploinsufficiency was obtained. In addition to a large set of genes regulated by the RAM transcription factor Ace2, genes related to cell wall biosynthesis, cell cycle, polarity, oxidative stress, and nitrogen utilization were identified. Follow-up analysis led to the first demonstration that the RAM pathway is required for oxidative stress tolerance in a manner related to the two-component-regulated kinase Chk1 and revealed a potential direct connection between the RAM pathway and the essential Mps1 spindle pole-related kinase. In addition, genetic interactions with CDC42-related genes MSB1, a putative scaffold protein, and RGD3, a putative Rho GTPase-activating protein (GAP) were identified. We also provide evidence that Rgd3 is a GAP for Cdc42 and show that its localization and phosphorylation are dependent on Cbk1.

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Mitotic Exit and Separation of Mother and Daughter Cells

Cited by 131 publications

References 394 publications

Analysis ofS. pombeSIN protein SPB-association reveals two genetically separable states of the SIN

Analysis ofS. pombeSIN protein SPB-association reveals two genetically separable states of the SIN

Dma1-dependent degradation of Septation Initiation Network proteins during meiosis inSchizosaccharomyces pombe

Complex Haploinsufficiency-Based Genetic Analysis of the NDR/Lats Kinase Cbk1 Provides Insight into Its Multiple Functions in Candida albicans

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