Mitotic exit: Determining the PP2A dephosphorylation program

Pereira, Gislene; Schiebel, Elmar

doi:10.1083/jcb.201608019

Cited by 5 publications

(5 citation statements)

References 12 publications

(32 reference statements)

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“…The structural subunit (PR65/A: 65 kDa), the regulatory subunit (B subunit: 50~130 kDa) and the catalytic subunit (PP2Ac: 36 ~ 38 kDa) form a trimer; this complex, is involved in the cell cycle, DNA replication, signal transduction, cell differentiation, malignant transformation and other cellular and biological events. PP2A was traditionally considered to be related to the occurrence and development of neurodegenerative diseases, tumors and other diseases 11 - 13 . Aberrant PP2A expression is associated with the occurrence and development of many malignant tumors, such as colorectal cancer, breast cancer, lung cancer and glioma 14 - 17 .…”

Section: Introductionmentioning

confidence: 99%

The prognostic value of Cyclin-Dependent Kinase 5 and Protein Phosphatase 2A in Gastric Cancer

Lin¹,

Xie²,

Weng³

et al. 2018

J. Cancer

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Purpose To discuss the relationship between the clinicopathological data, long-term survival of gastric cancer patients and different expression levels of Cyclin-Dependent Kinase 5 (CDK5) and Protein Phosphatase 2A (PP2A).Method The expression levels of CDK5 and PP2A were detected by immunohistochemistry in specimens from 124 patients with primary gastric cancer. The correlation among the expression of CDK5 and PP2A, clinicopathological factors and prognosis was investigated.Result The expression level of CDK5 was correlated with the TNM stage (p=0.030) and N stage (p=0.001), while the expression level of PP2A was correlated with the TNM stage and N stage (p=0.001 and p=0.004) as well as the degree of differentiation (p=0.046). The expression of CDK5 was positively correlated with the expression of PP2A in gastric cancer. Co-expression of CDK5 and PP2A is an independent prognostic factor that affected overall survival, and provided more accurate prognostic value for the overall survival of gastric cancer patients.Conclusion The expression of CDK5 and PP2A is positively correlated in gastric cancer. Co-expression of CDK5 and PP2A was an independent prognostic factor in patients with gastric cancer.

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Section: Introductionmentioning

confidence: 99%

The prognostic value of Cyclin-Dependent Kinase 5 and Protein Phosphatase 2A in Gastric Cancer

Lin¹,

Xie²,

Weng³

et al. 2018

J. Cancer

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show abstract

“…Entry into and progression through mitosis are driven by the phosphorylation of thousands of substrates under the master control of various mitotic kinases, such as CDK1 and PLK1 21 . However, phosphatase activities rise to cooperate with the declining activities of mitotic kinases to reset the phosphorylation status of proteins to the general low level that is characteristic of G1-phase cells 22 . Studies on yeast and human cells have revealed a timely defined dephosphorylation program that coordinates and regulates events in anaphase, telophase, and cytokinesis 23 – 25 .…”

Section: Discussionmentioning

confidence: 99%

iASPP–PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation

et al. 2018

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Cytokinesis is the last step of cell division and is concluded by the abscission of the intercellular bridge that connects two daughter cells. The tight regulation of cytokinesis completion is essential because cytokinesis failure is associated with various human diseases. Here, we report that iASPP, a member of the apoptosis-stimulating proteins of p53 (ASPP) family, is required for proper cell division. iASPP depletion results in abnormal midbody structure and failed cytokinesis. We used protein affinity purification methods to identify the functional partners of iASPP. We found that iASPP associates with centrosomal protein of 55 kDa (CEP55), an important cytokinetic abscission regulator. Mechanically, iASPP acts as a PP1-targeting subunit to facilitate the interaction between PP1 and CEP55 and to remove PLK1-mediated Ser436 phosphorylation in CEP55 during late mitosis. The latter step is critical for the timely recruitment of CEP55 to the midbody. The present observations revealed a previously unrecognized function of iASPP in cytokinesis. This function, in turn, likely contributes to the roles of iASPP in tumor development and genetic diseases.

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“…When the CENPF protein was increased, it prompted the production of AURKA kinase. Here, the rise of CDCA8 regulators will not only indirectly promote the production of AURKA kinase [14], but also directly affect it.…”

Section: Construction Of Cdtgrn Gene Regulatory Networkmentioning

confidence: 99%

CdtGRN: Construction of qualitative time-delayed gene regulatory networks with a deep learning method

Xu¹,

Zhang²,

Chen

2021

Preprint

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Background:Gene regulations often change over time rather than being constant. But many of gene regulatory networks extracted from databases are static. The tumor suppressor gene P 53 is involved in the pathogenesis of many tumors, and its inhibition effects occur after a certain period. Therefore, it is of great significance to elucidate the regulation mechanism over time points. Result:A qualitative method for representing dynamic gene regulatory network is developed, called CdtGRN. It adopts the combination of convolutional neural networks(CNN) and fully connected networks(DNN) as the core mechanism of prediction. The ionizing radiation Affymetrix dataset (E-MEXP-549) was obtained at ArrayExpress, by microarray gene expression levels predicting relations between regulation. CdtGRN is tested against a time-delayed gene regulatory network with 22, 284 genes related to P 53. The accuracy of CdtGRN reaches 92.07% on the classification of conservative verification set, and a kappa coefficient reaches 0.84 and an average AUC accuracy is 94.25%. This resulted in the construction of. Conclusion:The algorithm and program we developed in our study would be useful for identifying dynamic gene regulatory networks, and objectively analyze the delay of the regulatory relationship by analyzing the gene expression levels at different time points. The time-delayed gene regulatory network of P 53 is also inferred and represented qualitatively, which is helpful to understand the pathological mechanism of tumors.

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Mitotic exit: Determining the PP2A dephosphorylation program

Cited by 5 publications

References 12 publications

The prognostic value of Cyclin-Dependent Kinase 5 and Protein Phosphatase 2A in Gastric Cancer

The prognostic value of Cyclin-Dependent Kinase 5 and Protein Phosphatase 2A in Gastric Cancer

iASPP–PP1 complex is required for cytokinetic abscission by controlling CEP55 dephosphorylation

CdtGRN: Construction of qualitative time-delayed gene regulatory networks with a deep learning method

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