Mitotic Golgi disassembly is required for bipolar spindle formation and mitotic progression

Guizzunti, Gianni; Seemann, Joachim

doi:10.1073/pnas.1610844113

Cited by 45 publications

(49 citation statements)

References 64 publications

(80 reference statements)

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“…The exact molecular links that account for this hierarchical relationship are elusive, requiring further in‐depth investigation. Cells expressing ER‐ or GA‐targeted HRP and treated with DAB/H 2 O 2 exhibited local DAB precipitation before they died, indicating that ER and/or GA perturbations are sufficient for cell killing, perhaps due to the perturbation of GA trafficking (Jollivet et al , ) and dynamics (Guizzunti & Seemann, ).…”

Section: Discussionmentioning

confidence: 99%

Photodynamic therapy with redaporfin targets the endoplasmic reticulum and Golgi apparatus

et al. 2018

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Preclinical evidence depicts the capacity of redaporfin (Redp) to act as potent photosensitizer, causing direct antineoplastic effects as well as indirect immune-dependent destruction of malignant lesions. Here, we investigated the mechanisms through which photodynamic therapy (PDT) with redaporfin kills cancer cells. Subcellular localization and fractionation studies based on the physicochemical properties of redaporfin revealed its selective tropism for the endoplasmic reticulum (ER) and the Golgi apparatus (GA). When activated, redaporfin caused rapid reactive oxygen species-dependent perturbation of ER/GA compartments, coupled to ER stress and an inhibition of the GA-dependent secretory pathway. This led to a general inhibition of protein secretion by PDT-treated cancer cells. The ER/GA play a role upstream of mitochondria in the lethal signaling pathway triggered by redaporfin-based PDT Pharmacological perturbation of GA function or homeostasis reduces mitochondrial permeabilization. In contrast, removal of the pro-apoptotic multidomain proteins BAX and BAK or pretreatment with protease inhibitors reduced cell killing, yet left the GA perturbation unaffected. Altogether, these results point to the capacity of redaporfin to kill tumor cells via destroying ER/GA function.

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Section: Discussionmentioning

confidence: 99%

Photodynamic therapy with redaporfin targets the endoplasmic reticulum and Golgi apparatus

et al. 2018

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“…Prevention of ribbon disassembly, by interfering with the Golgi Reassembly Stacking Protein GRASP65 delays progression through the G2/M transition indicating that the change in Golgi morphology feeds into the cell cycle machinery pathway. Moreover, inhibition of the Golgi ribbon disassembly, by accumulation of a polymer of diaminobenzidene (DAB) in the Golgi lumen which inhibits membrane re‐modeling, arrests cells in early mitosis . The arrested cells lacked a bipolar spindle, had unseparated centrosomes, and had an active spindle assembly checkpoint.…”

Section: The Golgi As a Cell Sensormentioning

confidence: 99%

The Function of the Golgi Ribbon Structure – An Enduring Mystery Unfolds!

Gosavi

Gleeson

2017

BioEssays

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The Golgi apparatus in vertebrate cells consists of individual Golgi stacks fused together in a continuous ribbon structure. The ribbon structure per se is not required to mediate the classical functions of this organelle and the relevance of the "ribbon" structure has been a mystery since first identified ultrastructurally in the 1950s. Recent advances recognize a role for the Golgi apparatus in a range of cellular processes, some mediated by signaling networks which are regulated at the Golgi. Here we review the cellular processes and signaling events regulated by the Golgi apparatus and, in particular, explore an emerging theme that the ribbon structure of the Golgi contributes directly to the regulation of these higher order functions.

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“…He further showed that (Ferraro et al, 2016). Changes in Golgi organization, in particular its disassembly in early mitosis, also appear to have a role in controlling mitotic spindle assembly, as shown by Joachim Seemann (UT Southwestern, Dallas, USA) (Guizzunti and Seemann, 2016).…”

Section: Secretion: Making Proteins and Membranes And How To Get Themmentioning

confidence: 92%

Meeting report – Emerging Concepts in Cell Organization

Teis

Kukulski

2017

Journal of Cell Science

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New concepts in cell organization emerged in a medieval castle during a snowy week in January 2017 in the middle of the Austrian Alps. The occasion was the 10th Annaberg EMBO workshop in Goldegg am See; organized by Gabriele Seethaler, Catherine Rabouille and Marino Zerial. There were 95 participants, including many who gave talks and presented posters, enjoying a familial and trusting atmosphere that stimulated lively exchange of (unpublished) results, new ideas and thoughts.As at previous occasions when this community met in the same spot, the aim of the meeting was to identify the key questions in cell biology and set the ground for future research directions. The inspirational character of the meeting and the enthusiasm of the participants certainly achieved that. The new concepts that emerged will be shaping our understanding of how cells are organized and how this organization impacts cellular functions. Overall, it became clear that there is a discrepancy between the textbook views of the cellular building plan and how the field currently envisions a cell is really built. Furthermore, novel methods allow the study of the complexity of cellular organization, and old methods are being revisited. This report is an attempt to recapitulate and integrate the ideas that emerged at the workshop, and thereby provide inspiration to the larger community. All presentations and, in particular the lively poster sessions, were essential for the success of the meeting. However, owing to space restrictions, we could not cover all short talks or the posters here. We apologize to our colleagues whose presentations could not be discussed here.Secretion: making proteins and membranes and how to get them out A lot remains to be discovered about intra-organelle communication by 'classical' vesicular trafficking, especially in the secretory pathway. For instance, how do cells ensure that vesicles contain the right cargo? Liz Miller (MRC LMB, Cambridge, UK) explained protein quality control at endoplasmic reticulum (ER) exit sites and presented a cargo-crowding mechanism that helps to exclude ER-resident proteins from being packed into COPII vesicles, thereby preventing their 'spilling over' into the Golgi.While ER export has been intensely studied, exit sites from the trans-Golgi network (TGN) have remained more elusive. Stéphanie Miserey-Lenkei (Institut Curie, Paris, France) highlighted that RAB6 controls membrane fission of transport vesicles at the TGN through coupling motor proteins of the kinesin and myosin family to the cytoskeleton at specific sites of fission. This coupling ensures the spatial coordination between the fission of RAB6-positive vesicles and their exit along microtubules (Miserey-Lenkei et al., under review).Another important question is how is specificity in trafficking to and through the Golgi achieved? After all, the Golgi is the major protein distribution center of the cell. Sean Munro (MRC LMB, Cambridge, UK), this year's winner of the famous Grunzelsbacher award (sponsored by the elusive Aus...

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Mitotic Golgi disassembly is required for bipolar spindle formation and mitotic progression

Cited by 45 publications

References 64 publications

Photodynamic therapy with redaporfin targets the endoplasmic reticulum and Golgi apparatus

Photodynamic therapy with redaporfin targets the endoplasmic reticulum and Golgi apparatus

The Function of the Golgi Ribbon Structure – An Enduring Mystery Unfolds!

Meeting report – Emerging Concepts in Cell Organization

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