2002
DOI: 10.1128/mcb.22.3.874-885.2002
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Mitotic Phosphorylation of Histone H3: Spatio-Temporal Regulation by Mammalian Aurora Kinases

Abstract: Phosphorylation at a highly conserved serine residue (Ser-10) in the histone H3 tail is considered to be a crucial event for the onset of mitosis. This modification appears early in the G 2 phase within pericentromeric heterochromatin and spreads in an ordered fashion coincident with mitotic chromosome condensation. Mutation of Ser-10 is essential in Tetrahymena, since it results in abnormal chromosome segregation and extensive chromosome loss during mitosis and meiosis, establishing a strong link between sign… Show more

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Cited by 602 publications
(517 citation statements)
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References 61 publications
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“…As discussed above, expression of the Mcm2-7 proteins allows tumour cells engaged in the cell division cycle to be clearly distinguished from cells residing in out-of-cycle states. Geminin, which prevents relicensing of replication origins after the initiation of DNA synthesis, is only present in cells progressing through S, G 2 and M phases, as are the mitotic engine kinases Plk1, Aurora A and Aurora B [74,107,108]. These three kinases control most mitotic events, including centrosome maturation and separation, chromosome orientation and segregation [8].…”
Section: Geminin Mitotic Kinases and Phosphohistone H3 Can Be Used Tmentioning
confidence: 99%
See 1 more Smart Citation
“…As discussed above, expression of the Mcm2-7 proteins allows tumour cells engaged in the cell division cycle to be clearly distinguished from cells residing in out-of-cycle states. Geminin, which prevents relicensing of replication origins after the initiation of DNA synthesis, is only present in cells progressing through S, G 2 and M phases, as are the mitotic engine kinases Plk1, Aurora A and Aurora B [74,107,108]. These three kinases control most mitotic events, including centrosome maturation and separation, chromosome orientation and segregation [8].…”
Section: Geminin Mitotic Kinases and Phosphohistone H3 Can Be Used Tmentioning
confidence: 99%
“…These three kinases control most mitotic events, including centrosome maturation and separation, chromosome orientation and segregation [8]. Notably, histone H3 is a substrate for the Aurora kinases and is phosphorylated at serine 10 only during the length of M phase [108,109]. Phosphohistone H3 (H3S10ph) is therefore an M phase marker.…”
Section: Geminin Mitotic Kinases and Phosphohistone H3 Can Be Used Tmentioning
confidence: 99%
“…As shown in Figure 2, this was indeed the case. Fluorescence-activated cell sorting (FACS) analysis of cell populations doubly stained with propidium iodide and an antibody against the phosphorylated form of histone H3 (Xu et al 2001) allowed us to distinguish between G 2 -arrested and mitotic cells, as H3 is phosphorylated on Ser 10 only during mitosis (Crosio et al 2002). In the initial set of experiments (data not shown), we pretreated the cells with caffeine 30 min before adding MNNG and then incubated the cells for a further 24 or 48 h. Using this protocol, we failed to observe any differences between caffeine-treated and untreated cells, as measured by Western blotting with the phospho-H3 antibody, probably because the half-life of caffeine is only 4.5 h. We therefore added the kinase inhibitor some hours after the MNNG treatment.…”
Section: Caffeine and Ucn-01 Abrogate The Mmr-dependent G 2 Arrestmentioning
confidence: 99%
“…The presence of this modified histone has been correlated with mitotic chromosome condensation in several eukaryotic systems (Hendzel et al, 1997;Wei et al, 1998). H3 phosphorylation at Ser10 is governed by the activity of aurora/Ipl1 kinases (Speliotes et al, 2000;Giet and Glover 2001;Crosio et al, 2002) in competition with the phosphatase activity of the serine/threonine protein phosphatase 1 (Hsu et al, 2000;Murnion et al, 2001). It has been shown that Ser 10-modified histone colo-calizes with the human condensin complex during G2/ prophase (Schmiesing et al, 2000), suggesting that this modification may have a role in destabilizing local chromatin structure to allow access of condensation factors to DNA or in recruiting the condensation machinery on the modified histone amino-terminal tail (Wei et al, 1998;Schmiesing et al, 2000).…”
Section: Introductionmentioning
confidence: 99%