Mitotic proliferation of myoepithelial cells during regeneration of atrophied rat submandibular glands after duct ligation

Abstract

These findings suggest that myoepithelial cells are able to proliferate mitotically during regeneration of rat submandibular gland.

Biological behavior of myoepithelial cells in the regeneration of rat atrophied sublingual glands following release from duct ligation

“…According to Burgess et al (1996), observing the experimental atrophy and regeneration of the rat parotid gland, the proliferative activity of myoepithelial cells in atrophy is very high, however in regeneration it shows a 13 level similar to the controls. In the rat submandibular gland, myoepithelial cells both in atrophy and regeneration proliferate more actively than in normal glands (Takahashi et al 2001(Takahashi et al , 2004b. The previous reports above and the present study show that the three major salivary glands differ in the proliferative activity of myoepithelial cells under the induced pathological conditions.…”

“…Since myoepithelial cells participate in the histogenesis of several kinds of salivary gland tumors (Batsakis et al 1983, Dardick & Nostrand 1987, Redman 1994, it is possible to consider that the differences in proliferative activity of myoepithelial cells among the three major salivary glands are related to the differences in the frequencies and in the types of tumors of the salivary glands developing in the three glands. It has been speculated that the differences in the proliferative activity of myoepithelial cells originate in different distributions of myoepithelial cells (Takahashi et al 2001(Takahashi et al , 2004b or in different mechanisms of acinar cell deletion in the gland atrophy (Takahashi et al 2003). However, these two hypotheses are insufficient to explain the differences fully and there may be additional factors.…”

“…However, using double immunohistochemistry with actin and proliferating cell nuclear antigen (PCNA) Burgess et al (1996) found that myoepithelial cells are able to proliferate in the atrophy and regeneration of parotid glands, and the proliferative activity of myoepithelial cells has also been identified in atrophic (Takahashi et al 2001) and regenerative (Takahashi et al 2004b) submandibular glands and in atrophic sublingual glands (Takahashi et al 2003). There were differences in the degree of proliferative activity of myoepithelial cells among these cases, and it still remains unclear how myoepithelial cells proliferate in the regeneration of sublingual glands.…”

Isolation and Propagation of Mesenchymal Stem Cells from the Lacrimal Gland

“…19 -24 In particular, the regenerative capacity of the salivary glands has been well documented. 22,23,[25][26][27][28][29][30] Long-term (7-21 days) ligation of the main excretory ducts of the salivary glands leads to atrophy of the glands. The ligation of the salivary gland corresponds with inflammation, edema, and death of acinar cells.…”

Can Ocimum Basilicum (Basil) Relief the Impact of Chronic Unpredictable Mild Stress on the Mice Salivary Glands?

“…Another two set of the 4 microns-thick paraffin sections were processed for immunohistochemical examination using streptavidine-biotin-peroxidase technique according to Gu and Herrera 2010. Anti-alpha Smooth muscle actin (ASMA) antibody (Dako Cytomation, Heverlee, Belgium, with dilution of 1/1000), was used as a marker for identification of myoepithelial cells (MECs) (Takahashi et al, 2004). Anti-BDNF antibody (Santa Cruz Biotechnology, Texas, USA with dilutions of 1/400) was used to assess the immunohistochemical expression of this neurotrophin.…”