Mitoxantrone as a prospective photosensitizer for photodynamic therapy of breast cancer

Montazerabadi, Alireza; Sazgarnia, Ameneh; Bahreyni-Toosi, Mohammad Hossein; Ahmadi, Amirhossein; Shakeri‐Zadeh, Ali; Aledavood, Amir

doi:10.1016/j.pdpdt.2011.08.004

Cited by 39 publications

(28 citation statements)

References 27 publications

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“…In vitro research with use of various cell lines, breast cancer among them, confirm the direct cytotoxic influence of PDT [9][10][11][12]. Both necrosis and apoptosis were observed in cultures affected by a photosensitizer and then irradiated [12].…”

Section: Original Papersmentioning

confidence: 76%

“…However, there are continuous efforts to modify the method in order to use it also to fight deeper lesions, for example quite simple intratissue needles with fibre optics [22]. [9,10,11,14]. However, this does not explain the mechanism of the final effect of PDT, i.e.…”

Section: Discussionmentioning

confidence: 99%

“…Many research centres are currently focusing on assessing the effectiveness of various photosensitizers used during photodynamic reactions on in vitro breast cancer cells, including those from metastatic foci [9][10][11][12][13][14].…”

Section: Original Papersmentioning

confidence: 99%

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Expression of Proapoptotic BAX and TP53 Genes and Antiapoptotic BCL-2 Gene in MCF-7 and T-47D Tumour Cell Cultures of the Mammary Gland After a Photodynamic Therapy with Photolon

Płonka¹,

Latocha²,

Kuśmierz³

et al. 2015

Adv Clin Exp Med

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Background. Breast cancer is the most common malignant tumour in women in the whole world. Despite significant developments in the early diagnosis of breast cancer, there is no effective method which would assure total recovery of the patient. Currently available clinical data and laboratory tests indicate a possibility to introduce photodynamic therapy (PDT) to the supplementary treatment of breast cancer. Objectives. The aim of this study was to assess the influence of PDT with Photolon as a photosensibilizator on the expression of apoptosis associated genes (BCL-2, BAX, TP53) in human breast cancer cell lines, preceded by assessment of survivorship and proliferative activity in the tested cells after PDT. Material and Methods. In the present study human breast cancer cell lines MCF-7 and T-47D were used. Photolon (chlorin e6 complex: PVP 1:1) was used as a photosensitizer. Assessments of survivorship and proliferative activity of cells under the influence of PDT (WST-1 test) were conducted along with the expression of selected genes involved in the process of apoptosis: BCL-2, BAX, TP53 (RT-QPCR). Results. PDT limited both survivorship and proliferative activity of breast cancer cells in the two tested lines. In case of T-47D cell line was found increase of BAX and BCL-2 genes expression after PDT and sustained activity of TP53 gene. Conversely, in MCF-7 cell line a decrease in expression was found for both BAX and TP53 genes, but also an increase of BCL-2 gene expression. Conclusions. A progressing decrease (24, 48 and 72 h after PDT) in the count of culture cells, which suggests the occurrence of apoptosis initiated by a photodynamic reaction with simultaneous increase of BCL-2/BAX index, indicates activation of a different endogenous apoptosis pathway than the one examined, namely pointing to sui-

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Section: Original Papersmentioning

confidence: 76%

Section: Discussionmentioning

confidence: 99%

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Expression of Proapoptotic BAX and TP53 Genes and Antiapoptotic BCL-2 Gene in MCF-7 and T-47D Tumour Cell Cultures of the Mammary Gland After a Photodynamic Therapy with Photolon

Płonka¹,

Latocha²,

Kuśmierz³

et al. 2015

Adv Clin Exp Med

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“…10 Studies of PDT using MIT to treat breast cancer and melanoma cancer have been reported. 11,12 To conquer the MDR effect of MIT in breast cancer cells, using the photosensitizing property of MIT may exert unexpected anticancer effect due to the generation of ROS in cancer cells.…”

Section: Et Almentioning

confidence: 99%

“…Various concentrations of MIT-PFP/PPP mixed micelles (12.5, 25, 50, 100, 200 μM) were detected by UV-Visible Spectrophotometer to obtain the MIT light absorption spectra. 12 The experiments were carried out in triplicate.…”

Section: Absorption Properties Of Mitmentioning

confidence: 99%

Polymeric mixed micelles loaded mitoxantrone for overcoming multidrug resistance in breast cancer via photodynamic therapy

Li¹,

Cai²,

Zhao³

et al. 2017

IJN

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Mitoxantrone (MIT) is an anticancer agent with photosensitive properties that is commonly used in various cancers. Multidrug resistance (MDR) effect has been an obstacle to using MIT for cancer therapy. Photochemical internalization, on account of photodynamic therapy, has been applied to improve the therapeutic effect of cancers with MDR effect. In this study, an MIT-poly(ε-caprolactone)-pluronic F68-poly(ε-caprolactone)/poly( d , l -lactide-co-glycolide)–poly(ethylene glycol)–poly( d , l -lactide-co-glycolide) (MIT-PFP/PPP) mixed micelles system was applied to reverse the effect of MDR in MCF-7/ADR cells via photochemical reaction when exposed to near-infrared light. MIT-PFP/PPP mixed micelles showed effective interaction with near-infrared light at the wavelength of 660 nm and exerted great cytotoxicity in MCF-7/ADR cells with irradiation. Furthermore, MIT-PFP/PPP mixed micelles could improve reactive oxygen species (ROS) levels, decrease P-glycoprotein activity, and increase the cellular uptake of drugs with improved intracellular drug concentrations, which induced cell apoptosis in MCF-7/ADR cells under irradiation, despite MDR effect, as indicated by the increased level of cleaved poly ADP-ribose polymerase. These findings suggested that MIT-PFP/PPP mixed micelles may become a promising strategy to effectively reverse the MDR effect via photodynamic therapy in breast cancer.

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Early Detection of Cancer by Laser Therapy

2024

Laser Therapy in Healthcare

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Mitoxantrone as a prospective photosensitizer for photodynamic therapy of breast cancer

Cited by 39 publications

References 27 publications

Expression of Proapoptotic BAX and TP53 Genes and Antiapoptotic BCL-2 Gene in MCF-7 and T-47D Tumour Cell Cultures of the Mammary Gland After a Photodynamic Therapy with Photolon

Expression of Proapoptotic BAX and TP53 Genes and Antiapoptotic BCL-2 Gene in MCF-7 and T-47D Tumour Cell Cultures of the Mammary Gland After a Photodynamic Therapy with Photolon

Polymeric mixed micelles loaded mitoxantrone for overcoming multidrug resistance in breast cancer via photodynamic therapy

Early Detection of Cancer by Laser Therapy

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