Mix‐and‐match nanobiosensor design: Logical and spatial programming of biosensors using self‐assembled DNA nanostructures

Liu, Ying; Kumar, Sriram; Taylor, R. E.

doi:10.1002/wnan.1518

Cited by 18 publications

(12 citation statements)

References 169 publications

(288 reference statements)

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“…Like a "Swiss army knife" with many capabilities, DNA nanotechnology has the potential to create multivalent, dynamic and responsive gene delivery platforms capable of unprecedented control over targeting and delivery while integrating multiple distinct approaches using CPPs [134,135]. However, for optimization of this technology, robust and low-cost stabilization methods are critical for protecting DNA origami from low salt denaturation and enzymatic degradation in vivo.…”

Section: Dna Origamimentioning

confidence: 99%

Cell Penetrating Peptides, Novel Vectors for Gene Therapy

2020

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Cell penetrating peptides (CPPs), also known as protein transduction domains (PTDs), first identified ~25 years ago, are small, 6–30 amino acid long, synthetic, or naturally occurring peptides, able to carry variety of cargoes across the cellular membranes in an intact, functional form. Since their initial description and characterization, the field of cell penetrating peptides as vectors has exploded. The cargoes they can deliver range from other small peptides, full-length proteins, nucleic acids including RNA and DNA, liposomes, nanoparticles, and viral particles as well as radioisotopes and other fluorescent probes for imaging purposes. In this review, we will focus briefly on their history, classification system, and mechanism of transduction followed by a summary of the existing literature on use of CPPs as gene delivery vectors either in the form of modified viruses, plasmid DNA, small interfering RNA, oligonucleotides, full-length genes, DNA origami or peptide nucleic acids.

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Section: Dna Origamimentioning

confidence: 99%

Cell Penetrating Peptides, Novel Vectors for Gene Therapy

2020

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“… 41 These functionalizations lie at the heart of some of the technology's most promising applications: to spatially organize countless biological and synthetic materials and particles with nanometer precision in periodic arrays or anisotropic arbitrarily shaped nanostructures. This makes DNA nanotechnology an area of particular interest in biosensing, 29 single-molecule studies, 37,42–45 molecular force sensors, 40,46 nanophotonics, 47 drug delivery, 48 and other fields. Similarly, lipid membrane applications often rely on functionalizations, as we will explain in Sec.…”

Section: Dna Nanotechnologymentioning

confidence: 99%

Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics

et al. 2020

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DNA nanotechnology has proven exceptionally apt at probing and manipulating biological environments as it can create nanostructures of almost arbitrary shape that permit countless types of modifications, all while being inherently biocompatible. Emergent areas of particular interest are applications involving cellular membranes, but to fully explore the range of possibilities requires interdisciplinary knowledge of DNA nanotechnology, cell and membrane biology, and biophysics. In this review, we aim for a concise introduction to the intersection of these three fields. After briefly revisiting DNA nanotechnology, as well as the biological and mechanical properties of lipid bilayers and cellular membranes, we summarize strategies to mediate interactions between membranes and DNA nanostructures, with a focus on programmed delivery onto, into, and through lipid membranes. We also highlight emerging applications, including membrane sculpting, multicell self-assembly, spatial arrangement and organization of ligands and proteins, biomechanical sensing, synthetic DNA nanopores, biological imaging, and biomelecular sensing. Many critical but exciting challenges lie ahead, and we outline what strikes us as promising directions when translating DNA nanostructures for future in vitro and in vivo membrane applications.

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“…This result also indicates that microswimmers of this design can be expanded to cover a larger area with instantaneous control. As DNA can be a viable platform for both sensing and response [20], expansion in this manner could reveal a section of DNA designed to respond to environmental markers to trigger an event. Furthermore, if this design were made larger with multiple microspheres and linkers, a much larger area could be covered, potentially blocking passage through a channel or capturing an object in the environment for transport or sequestering.…”

Section: Expansion In a Constant Magnitude Rotating Magnetic Fieldmentioning

confidence: 99%

A Customizable DNA and Microsphere-Based, Magnetically Actuated Microswimmer

Harmatz

Travers

Taylor

2020

J. Microelectromech. Syst.

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Locomoting microscale robots-microswimmershave the potential to impact numerous applications due to their ability to selectively interact with their environment with microscale control. Previous microswimmer designs lack either submicron-level precision over their construction or instantaneous control over their shape. Thus, existing microswimmer designs limit the control afforded over microswimmer interactions with their environment. This work presents a magnetically actuated microswimmer constructed from DNA and microspheres in a hybrid top-down and bottom-up assembly technique that allows for submicron-level precision over microswimmer body construction. Microscale features were fabricated via two-photon polymerization, from which polydimethylsiloxane templates were molded. Templated assembly by selective removal was used to deposit microspheres of select size and composition into the templates. This technique was confirmed to be sizeselective within a population of microspheres. To construct the microswimmer, a ferromagnetic microsphere and a nonmagnetic microsphere were deposited into a template, connected with DNA nanotubes in their arrangement, magnetized, and pulled from the template. We demonstrate instantaneous control over shape changes via expansion and contraction of a microswimmer's body in an external magnetic field. In a rotating magnetic field of constant magnitude, the microswimmer expanded as the two microspheres separated a distance of 4.1±0.5 micrometers, approximately the distance between microspheres deposited in the template. In an oscillating magnetic field, the same microswimmer contracted and locomoted 58.8±0.7 micrometers over 126 seconds.

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Mix‐and‐match nanobiosensor design: Logical and spatial programming of biosensors using self‐assembled DNA nanostructures

Cited by 18 publications

References 169 publications

Cell Penetrating Peptides, Novel Vectors for Gene Therapy

Cell Penetrating Peptides, Novel Vectors for Gene Therapy

Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics

A Customizable DNA and Microsphere-Based, Magnetically Actuated Microswimmer

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