Mixed culture biotechnology and its versatility in dark fermentative hydrogen production

Mohanakrishna, Gunda; Pengadeth, Devu

doi:10.1016/j.biortech.2023.130286

Cited by 18 publications

(3 citation statements)

References 132 publications

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“…After the heat shock, the sludge was cooled down to 40 °C. Mohanakrishna and Pengadeth reported that heat shocks are often performed in the range of 65 to 121 °C and with exposure times between 1 to 10 h [18]. In the present work, the temperature of the heat shock is up to 80 °C ± 1 °C and the exposure times only at 5-10 min for the microwave and 10-20 min for the oven pre-treatment.…”

Section: Methodsmentioning

confidence: 82%

Microwave assisted acidification for green hydrogen and organic acids production

Barth,

Werner,

Otto

et al. 2023

Preprint

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Heat shocks are a favoured method used to trigger hydrogen production in dark fermentation. However, these treatments are often restricted to a very small volume, which is then inoculated into synthetic medium. Moreover, heat shocks are usually applied with an oven. This study explored the feasibility of applying heat shocks to the entire seed sludge using a microwave, a method not previously investigated. The experiments were conducted on a technical scale with 5 litre tank reactors. The highest productivity was achieved with an oven (0.95 mol H2/mol hexose), but the microwave was not significantly different and had the advantage of heating the sludge 6.4 times faster. The study also emphasized the repeatability of heat shocks, with microwave-assisted experiments showing low variation coefficients averaging 29%. The approach is already economically sustainable with high-temperature heat pumps with a COP of 4.3, enhancing its practical relevance.Highlights-The entire reactor content of 3.5 litre was heat shocked.-Heat shocks economically boosted dark fermentation hydrogen yield.-Heat pumps with COP of 4.3 would allow energetic net gain due to heat shocks.-Oven and microwave-based heat shocks are not significantly different.-Microwaves heat sludge 6.4 times faster than ovens.-Heat shocks inhibited methane production completely.-Heat shocks increased particularlyClostridium sensu stricto1 and 11.-Hydrogen could be enriched from sucrose, but not from lipids or proteins.

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Section: Methodsmentioning

confidence: 82%

Microwave assisted acidification for green hydrogen and organic acids production

Barth,

Werner,

Otto

et al. 2023

Preprint

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“…However, a biomass residue without heavy metals is what remains in this process of the mixture of the E. crassipes plant and biosolid (WTRs) and has potential as a biofertilizer due to its physicochemical characteristics. The anaerobic fermentation of organic matter produces an organic residue with exceptional fertilizing properties, comprising an average of 8.5% organic matter, 2.6% nitrogen, 1.5% phosphorus, 1.0% potassium, and a pH of 7.5 [58][59][60][61][62][63][64]. The material used with heavy metals must finally be disposed of as hazardous waste.…”

Section: Production Of Biohidrogenmentioning

confidence: 99%

The Design of a Sustainable Industrial Wastewater Treatment System and The Generation of Biohydrogen from E. crassipes

Sayago

2024

Polymers

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Water scarcity is a significant global issue caused by the prolonged disregard and unsustainable management of this essential resource by both public and private bodies. The dependence on fossil fuels further exacerbates society’s bleak environmental conditions. Therefore, it is crucial to explore alternative solutions to preserve our nation’s water resources properly and promote the production of biofuels. Research into the utilization of E. crassipes to remove heavy metals and generate biofuels is extensive. The combination of these two lines of inquiry presents an excellent opportunity to achieve sustainable development goals. This study aims to develop a sustainable wastewater treatment system and generate biohydrogen from dry, pulverized E. crassipes biomass. A treatment system was implemented to treat 1 L of industrial waste. The interconnected compartment system was built by utilizing recycled PET bottles to generate biohydrogen by reusing the feedstock for the treatment process. The production of biological hydrogen through dark fermentation, using biomass containing heavy metals as a biohydrogen source, was studied. Cr (VI) and Pb (II) levels had a low impact on hydrogen production. The uncontaminated biomass of E. crassipes displayed a significantly higher hydrogen yield (81.7 mL H2/g glucose). The presence of Cr (IV) in E. crassipes leads to a decrease in biohydrogen yield by 14%, and the presence of Pb (II) in E. crassipes leads to a decrease in biohydrogen yield of 26%. This work proposes a strategy that utilizes green technologies to recover and utilize contaminated water. Additionally, it enables the production of bioenergy with high efficiency, indirectly reducing greenhouse gases. This strategy aligns with international programs for the development of a circular economy.

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“…Bacillus, lactic acid bacteria or enterobacteria) performing complementary functions like aggregation or detoxification (Cabrol et al, 2017;Lopez-Hidalgo et al, 2022). Other advantages in terms of mixed consortia in hydrogen production include easiness in bioreactor management and substrate utilization but the fact that ecological interactions are not understood makes it difficult to predict an improve their performance (Mohanakrishna and Pengadeth, 2024). Pearson correlations between microbial abundance, biogas production and volatile fatty acids concentration in the non-treated bioreactors (n=120).…”

Section: Treatments On Inoculum Predictably Affected the Diversity St...mentioning

confidence: 99%

Levels of microbial diversity affect the stability and function of dark fermentation bioreactors

Navarro-Díaz,

Aparicio-Trejo,

Valdez-Vazquez

et al. 2024

Front. Ind. Microbiol.

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Climate change and pollution drive the need for fossil fuel alternatives. Dark fermentation offers promise through the use of microbial consortia to convert organic matter into hydrogen gas. Persisting challenges like instability and low yields may stem from reduced diversity of the anaerobic digestion communities that serve as inoculum and undergo aggressive pretreatments and culturing conditions. This study explores the impact of diversity loss on function, focusing on biogas production and stability. Two treatments, with and without aggressive pretreatment, were tested on 12 replicate bioreactors each, resulting in differing microbial diversity levels. Microbial communities were assessed via 16S amplicon sequencing, monitoring biogas production, volatile fatty acids, and testing invasion susceptibility. The two treatments exhibited divergent assembly and functional trajectories, although replicates within each treatment ultimately converged into similar compositions and stable levels of biogas production. Heat-treated bioreactors showed a 91.5% biogas increase but exhibited higher invasion susceptibility compared to non-treated. Non-treated bioreactors showed unique species associations with biogas production (e.g. Ethanoligenens harbinense and Enterococcus olivae), distinct from the commonly studied Clostridium group. These findings provide insights into the effects of diversity loss on stability, elucidating differences across taxonomic and functional stability as well as invasion susceptibility. Moreover, the identification of novel bacterial groups associated with hydrogen production suggests promising directions for future research to enhance microbial consortia control and design in dark fermentation.

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Mixed culture biotechnology and its versatility in dark fermentative hydrogen production

Cited by 18 publications

References 132 publications

Microwave assisted acidification for green hydrogen and organic acids production

Microwave assisted acidification for green hydrogen and organic acids production

The Design of a Sustainable Industrial Wastewater Treatment System and The Generation of Biohydrogen from E. crassipes

Levels of microbial diversity affect the stability and function of dark fermentation bioreactors

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