Mixed culture fermentation between Rhizopus nigricans and Trichoderma pseudokoningii to control cucumber Fusarium wilt

Cong, Yunzhe; Fan, Hengda; Ma, Qiushuang; Lu, Yan; Xu, Lei; Zhang, Pengying; Chen, Kaoshan

doi:10.1016/j.cropro.2019.104857

Cited by 26 publications

(22 citation statements)

References 44 publications

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“…The mixed fermentation of micro-organisms can increase the yield of known or unknown bioactive constituents, and even the production of new metabolites, which was determined to be a good means to discover and apply natural products (Pettit, 2009). Our previous studies found that MCF containing Trichoderma pseudokoningii and Rhizopus nigricans can effectively control cucumber Fusarium wilt (Cong et al, 2019), and prolong the shelf life and improve the quality of kiwi fruit (Ma et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Each flowerpot contained four plants. After 30 days of seed germination, the plants were inoculated using the method of root injury treatment (Cong et al, 2019) with slight modifi--cations, in which a wound of 0.5 cm in the roots was made. Tomato seedlings were carefully uprooted, the root soil was removed, and then, the roots were soaked in 10 8 CFU per ml of F. oxysporum f. sp.…”

Section: Pot Experimentsmentioning

confidence: 99%

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Effects of mixed culture fermentation of Bacillus amyloliquefaciens and Trichoderma longibrachiatum on its constituent strains and the biocontrol of tomato Fusarium wilt

Cong

Feng

et al. 2021

J of Applied Microbiology

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To study the effects of mixed culture fermentation (MCF) of Bacillus amyloliquefaciens and Trichoderma longibrachiatum on its constituent strains and the application values for agricultural production, with the intention of developing efficient and environmentally friendly biocontrol agents. Methods and results: In this study, an in vitro antifungal growth experiment showed that the inhibitory rate of the MCF broth on pathogenic fungi (Fusarium oxysporum f. sp. lycopersici, Botrytis cinerea, Trichothecium roseum and Colletotrichum gloeosporioides) was less than that of B. amyloliquefaciens culture fermentation (BCF). Moreover, the content and gene expression of lipopeptide antibiotics were also lower than that in the BCF group. However, the pot experiments based on irrigation with appropriately diluted fermentation broth showed that the biocontrol effect of MCF on tomato Fusarium wilt was significantly higher than that of TCF (T. longibrachiatum culture fermentation) and BCF, and was approximately 15.79% higher than that of the BTF group which made by mixing equivalent amounts of BCF and TCF. In MCF broth, two micro-organisms antagonized and coexisted, and the growth of T. longibrachiatum was inhibited. Using transcriptomic analysis, we speculated that MCF can upregulate the expression of genes related to carbon and nitrogen metabolism, oxidation-reduction activity, sporulation, environmental information response and chemotaxis, and biosynthesis of secondary metabolites of B. amyloliquefaciens, which might enhance the nutrient substances metabolism and competitiveness, survival ability, colonization and adaptability to the environment to increase its biocontrol potential. Conclusions: Mixed culture fermentation could promote the more reasonable and effective utilization of biocontrol micro-organisms though improving biocontrol effect, enhancing strains survival and competitiveness, increasing beneficial metabolites, combined with resistance induction or synergistic control. Significance and Impact of the Study: Using MCF agronomically utilizes biocontrol agents in an efficient way, which has a good potential for commercial implementation and could reduce production costs.

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Section: Discussionmentioning

confidence: 99%

Section: Pot Experimentsmentioning

confidence: 99%

Effects of mixed culture fermentation of Bacillus amyloliquefaciens and Trichoderma longibrachiatum on its constituent strains and the biocontrol of tomato Fusarium wilt

Cong

Feng

et al. 2021

J of Applied Microbiology

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“…B 12 was produced by mixed fermentation of Propionibacterium freudenreichii and L. brevis (Xie et al 2019 ); Dairy products (Kongo et al 2006 ) and lactic acid (Zhang and Vadlani 2015 ) was produced by co-fermentation of B. animalis and L. acidophilus . Cong et al ( 2019 ) used the mixed fermentation broth of R. nigricans and Trichoderma pseudokoningii to control cucumber wilt, and found that the combined fermentation has a synergistic effect on the control of Fusarium oxysporum . Based on the characteristics of C. butyricum and B. coagulans , a green and energy-saving co-fermentation process was established in this study.…”

Section: Discussionmentioning

confidence: 99%

Optimization of an economical medium composition for the coculture of Clostridium butyricum and Bacillus coagulans

Wang

Liu

et al. 2022

AMB Expr

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Clostridium butyricum is a spore-forming probiotic which can promote the enhancement of beneficial bacteria and maintain intestinal microecological balance. However, it is difficult to improve the production level of C. butyricum by conventional fermentation process. In this study, a co-fermentation process of C. butyricum DL-1 and Bacillus coagulans ZC2-1 was established to improve the viable counts and spore yield of C. butyricum, and the formula of coculture medium was optimized by flask fermentation. The results showed that the optimum medium composition is 10 g/L bran, 15 g/L corn steep powder, 15 g/L peptone, 1 g/L K2HPO4 and 0.5 g/L MnSO4.Cultured stationarily in the optimal medium for 36 h, the number of viable bacteria of C. butyricum DL-1 reached 1.5 × 108 cfu/mL, Which was 375 times higher than that incubated in the initial medium. The sporulation rate reach 92.6%. The results revealed an economical and effective medium composition for the coculture of C. butyricum and B. coagulans, which achieved a 64.6% cost reduction. The co-fermentation process established in this study provides a new fermentation mode for the industrial production of other absolute anerobic bacteria.

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“…As expected, the relative abundance of Bacillus increased with PBB treatment. In addition, some potential beneficial genera were also enriched, including Mucor, Rhizopus, Cunninghamella, Chitinophaga and Phenylobacterium [28][29][30][31][32]. Nine genera were significantly decreased, including Fusarium, which includes pathogens that can cause a variety of crop diseases [15,16].…”

Section: Discussionmentioning

confidence: 99%

A Novel Bio-Fertilizer Produced by Prickly Ash Seeds with Biochar Addition Induces Soil Suppressiveness against Black Shank Disease on Tobacco

et al. 2021

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A novel bio-fertilizer, produced from prickly ash seeds (PAS), Bacillus subtilis and biochar, was evaluated for its disease-preventing potential on tobacco black shank caused by Phytophthora nicotianae. The results showed that biochar promoted the growth of Tpb55 in PAS and increased the pH of the organic fertilizer. The final concentration of B. subtilis could reach 1.7 × 1010 cfu g−1 in the biological organic fertilizer (PBB) under the optimal medium under conditions of solid-state fermentation. PBB exhibited a strong fumigation effect on P. nicotianae, including inhibiting mycelium growth, reducing the disease severity and decreasing the pathogen population in rhizospheric soil. PBB treatment also could significantly increase the pH of acidified soil and improve soil nutrition content such as available K, alkali hydrolysable N and organic carbon. High-throughput pyrosequencing of 16S and 18S rRNA genes revealed that 4% PBB addition in soil had significant effects on the diversity and richness of fungi but not on that of bacteria. The microbial community structure was also shifted after PBB treatment. Some potentially beneficial microbes such as Bacillus, Mucor, Cunninghamella, Chitinophaga and Phenylobacterium were enriched, while potential pathogen Fusarium was significantly decreased. In conclusion, the agricultural waste PAS combined with biochar can replace soybean as a source for the production of biocontrol B. subtilis Tpb55, and the novel bio-fertilizer could effectively control tobacco black shank by pathogen inhibition, soil nutrient improvement and shifting the rhizomicrobial community.

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Mixed culture fermentation between Rhizopus nigricans and Trichoderma pseudokoningii to control cucumber Fusarium wilt

Cited by 26 publications

References 44 publications

Effects of mixed culture fermentation of Bacillus amyloliquefaciens and Trichoderma longibrachiatum on its constituent strains and the biocontrol of tomato Fusarium wilt

Effects of mixed culture fermentation of Bacillus amyloliquefaciens and Trichoderma longibrachiatum on its constituent strains and the biocontrol of tomato Fusarium wilt

Optimization of an economical medium composition for the coculture of Clostridium butyricum and Bacillus coagulans

A Novel Bio-Fertilizer Produced by Prickly Ash Seeds with Biochar Addition Induces Soil Suppressiveness against Black Shank Disease on Tobacco

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