Mixed ferrocene-glucose oxidase-carbon-paste electrode for amperometric determination of glucose

Wang, Joseph; Wu, Li-Huey; Lu, Zhenhuan; Li, Ruiliang; Sanchez, Juanita

doi:10.1016/s0003-2670(00)80501-2

Cited by 132 publications

(34 citation statements)

References 14 publications

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“…In the initial study [3], the ferrocene derivative was adsorbed at the surface of the electrode and was prevented to leach in the solution on solubility grounds. Another approach based on the same concept of insolubility of the redox mediator, ferrocenes and quinones were immobilized in carbon paste electrodes [10,11,27]. However, leakage of the mediators cannot be avoided completely because these species have a ®nite solubility in aqueous solution.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical and Enzymatic Studies of Electron Transfer Mediation by Ferrocene Derivatives with Nafion-Glucose Oxidase Electrodes

et al. 1999

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Na®on-glucose oxidase-ferrocene electrodes have been used to study the electron transfer between an electrode and the active site of the enzyme. These electrodes were prepared by casting a mixture of Na®on and glucose oxidase, GOx, (both dissolved in methanol) at the surface of a platinum disk electrode. The ferrocene mediators were incorporated by either soaking PtyNa®on and PtyNa®on-GOx electrodes in an aqueous solutions of N,N H -dimethylaminomethylferrocene, DMAFc, or by depositing an aliquot of a ferrocene solution at the surface of the enzyme ®lm electrode. The Na®on-GOx electrodes are characterized by a slower incorporation rate of the ferrocene than the plain Na®on electrode indicating that the af®nity of Na®on for the ferrocene derivative is increased by the addition of the enzyme. Accordingly, the ion-exchange distribution coef®cient is larger for plain Na®on (3.2610 3 ) than for Na®on-GOx (7.2610 2 ). The cyclic voltammetry of the PtyNa®on-GOx electrode in the presence of glucose and in deaerated solution show a well developed catalytic wave indicating that the ferrocene acts as a mediator for the electron transfer between the enzyme and the platinum electrode. For a high glucose concentration (75 mM), the amperometric response of these electrodes increased and the apparent diffusion coef®cient DMAFc decreased with an increase of DMAFc concentration in the enzymatic layer. These observations suggest that the glucose response is limited by the probability of an encounter between the reduced enzyme and the oxidized ferrocene rather than by the charge transfer between the reduced ferrocene and the electrode. The effective Michaelis constant, K m , for glucose and DMAFc were evaluated and values of 25 mM and 4 nmolycm 2 were obtained, respectively.

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Section: Introductionmentioning

confidence: 99%

Electrochemical and Enzymatic Studies of Electron Transfer Mediation by Ferrocene Derivatives with Nafion-Glucose Oxidase Electrodes

et al. 1999

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“…33 Carbon-related materials have been investigated earlier as a sensor material due to their high potential abilities in terms of both surface area and electron mobility. In consideration of this point, our data obtained for the InP porous nanostructures was not bad for an initial attempt.…”

Section: Sensitivity and Stability Of Inp Porous Sensors-as Shown Inmentioning

confidence: 99%

Electrochemical Functionalization of InP Porous Nanostructures with a GOD Membrane for Amperometric Glucose Sensors

Sato

Mizohata

Hashizume

2010

J. Electrochem. Soc.

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The electrochemical functionalization of n-type InP porous nanostructures and their feasibility for biochemical sensor applications were investigated. The porous structures have extremely large surface areas, i.e., over 10 m 2 /cm 3 , and superior electrical properties with conductive semiconductor substrates. As a first attempt at electrochemical functionalization, we successfully deposited a glucose oxidase ͑GOD͒ membrane onto an InP surface under an applied anodic bias of 1.2 V. With the addition of glucose, the response currents on the porous electrodes increased compared to those on planar InP electrodes due to their enlarged surface area. The sensitivity curves of the porous electrodes we used showed good linearity between the response currents and concentrations in a range from 0 to 5 mM.

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“…One promising avenue to the production of reagentless enzyme electrodes is to incorporate the biocatalyst within a carbon paste matrix [23][24][25]. Numerous studies have demonstrated the versatility, stability, and ease of operation of chemically modified carbon paste electrodes.…”

Section: Biological Modificationmentioning

confidence: 99%

“…We have investigated the simultaneous incorporation of glucose oxidase and various ferrocene derivatives into a carbon paste [24]. Repetitive measurements with a paste containing glucose oxidasdl ' 1-dimethylferrocene yielded a relative standard deviation of 3%.…”

Section: Biological Modificationmentioning

confidence: 99%

Modified electrodes for electrochemical sensors

Wang

1991

Electroanalysis

Self Cite

170

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Chemically modified electrodes exhibit unique behavior that can greatly benefit electrochemical sensing. In this article we review the development and applications of modified electrodes for chemical sensing. After a general introduction, different strategies for surface modification, as employed in chemical sensing, are discussed in detail. Special attention is given to permselective coatings, which offer controlled access at the sensor surface or the surface immobilization of biological entities that specifically recognize the analyte. Better understanding of these modified electrodes is achieved by using high resolution surface characterization techniques. Future prospects are evaluated. mTRODUCTIOiVThe development of electrochemical sensors continues to be a rapidly growing area of electrochemistry. Improvements in the stability, selectivity, and scope of such sensors are highly desirable to meet new challenges posed by clinical and environmental samples. The utility of solidelectrode-based sensors is often hampered by a gradual fouling of the surface due to adsorption of large organic surfactants or of reaction products. Amperometric sensors lack the ability to discriminate between solutes possessing similar redox characteristics. Finally, the sensing of many important solutes is often hindered by their slow electron transfer kinetics at the commonly used electrodes materials.One field that offers great potential for alleviating the problems above, hence for enhancing the power and scope of electrochemical sensors, is that comprising chemically modified electrodes (CMEs) [ 1, 21. The ability to deliberately control and manipulate the surface properties can lead to a variety of attractive effects. Such tailoring of the surface can meet the needs of many sensing problems. The field of CMEs, which has experienced a period of rapid growth over the past decade, has now reached a level of maturity that allows the use of these electrodes for routine sensing applications. SURFACE-MODIFID AMPEROMETHIC SENSORSThe most common approaches for incorporating a modifier into surfaces of amperometric sensors have been coverage with an appropriate polymer film and doping of a carbon paste. The modifier should be firmly attached to the surface, and it should not dissolve in the surrounding solution. The long-term stability of the attached moieties is a key factor for the performance of reliable sensors. Stability of days or weeks is essential to achieve widespread acceptance.The preparation of structure interfaces of deliberately designed character offers many promising prospects with regard to the development of chemical sensors. There are different directions by which the resulting CMEs can benefit sensing applications. These include acceleration of electron transfer reactions, prevention of fouling, permselective transport, preferential accumulation, and biocatalysis. Chemical and biological surface modifiers can thus enhance the selectivity, sensitivity, and scope of electrochemical sensors. The following sections...

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Mixed ferrocene-glucose oxidase-carbon-paste electrode for amperometric determination of glucose

Cited by 132 publications

References 14 publications

Electrochemical and Enzymatic Studies of Electron Transfer Mediation by Ferrocene Derivatives with Nafion-Glucose Oxidase Electrodes

Electrochemical and Enzymatic Studies of Electron Transfer Mediation by Ferrocene Derivatives with Nafion-Glucose Oxidase Electrodes

Electrochemical Functionalization of InP Porous Nanostructures with a GOD Membrane for Amperometric Glucose Sensors

Modified electrodes for electrochemical sensors

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