Mixed Genotypes in Hepatitis C Virus Infection

Baré, Patricia; Bianco, R.

doi:10.5772/28111

Cited by 3 publications

(3 citation statements)

References 52 publications

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“…13 The clinical repercussion of this mixed infection is that it might worsen liver damage and accelerate the progression of liver disease. [14][15] This particular patient denied any high risk behaviour during questionnaire session.…”

Section: Discussionmentioning

confidence: 93%

Study of Hepatitis C Virus Infection at a Tertiary Hospital: Genotyping, Risk Factors and Comorbidities

Fazlin¹,

Aini²,

Hadzri³

et al. 2017

imjm

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Introduction: Hepatitis C virus (HCV) genotyping is very important for the clinical management of HCVinfected patients. The aim of this study was to determine the genotypes of HCV-infected patients and to identify their risk factors and comorbidities. Materials and Methods: This was an observational, cross sectional study in which forty (40) HCV-infected patients attending Gastroenterology Clinic, Hospital Tengku Ampuan Afzan (HTAA) Kuantan Pahang were recruited for the study, from January to July 2014. Nucleotide sequence analysis of the 5’UTR and NS5B region were performed to identify the viral genotypes. Results: Of the 40 samples, 31 (77.5%) isolates were successfully classified into their genotypes and subtypes; 3a (57.5%), 1a (12.5%), 3b (2.5%) and 1b (2.5%). No genotype 2, 4, 5 and 6 were found in this study. However, there was one mixed-genotype (3a/1a) HCV infection. Risk factors and co-morbidities found in this study include IVDUs, haemodialysis, blood transfusion, surgery and co-infection with HIV. Conclusion: Genotype 3 followed by genotype 1 were the common HCV genotypes found in this study population. Furthermore, the highest risk factors and co-morbidities were IVDUs and co-infection with HIV.

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Section: Discussionmentioning

confidence: 93%

Study of Hepatitis C Virus Infection at a Tertiary Hospital: Genotyping, Risk Factors and Comorbidities

Fazlin¹,

Aini²,

Hadzri³

et al. 2017

imjm

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show abstract

“…We found a higher percentage of mixed infections than previously reported in this same cohort perhaps because the present study was a longitudinal analysis with serial plasma samples studied for each patient. The analysis of serial plasma samples instead of one single plasma sample increased the chance of discovering mixed infections by 5.2 times .…”

Section: Resultsmentioning

confidence: 99%

Hepatitis C virus long‐term persistence in peripheral blood mononuclear cells in patients with haemophilia. Detection of occult genotype 1

Parodi

García

Monzani

et al. 2014

Journal of Viral Hepatitis

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Peripheral blood mononuclear cells (PBMC) from chronic hepatitis C virus-infected persons can harbour viral variants that are not detected in plasma samples. We explored the presence and persistence of HCV genotypes in plasma and PBMC cultures from 25 HCV-monoinfected and 25 HIV/HCV-coinfected patients with haemophilia. Cell cultures were performed at different time points between 1993 and 2010-2011, and the HCV genome was examined in culture supernatants. Sequential plasma samples were studied during the same time period. Analysing sequential plasma samples, 21% of patients had mixed-genotype infections, while 50% had mixed infections determined from PBMC culture supernatants. HIV coinfection was significantly associated with the presence of mixed infections (OR = 4.57, P = 0.02; 95% CI = 1.38-15.1). In our previous study, genotype 1 was found in 72% of 288 patients of this cohort. Similar results were obtained with the sequential plasma samples included in this study, 69% had genotype 1. However, when taking into account plasma samples and the results from PBMC supernatants, genotype 1 was identified in 94% of the population. The PBMC-associated variants persisted for 10 years in some subjects, emphasizing their role as long-term reservoirs. The presence of genotype 1 in PBMC may be associated with therapeutic failure and should not be disregarded when treating haemophilic patients who have been infected by contaminated factor concentrates. The clinical implications of persistent lymphotropic HCV variants deserve further examination among multiple exposed groups of HCV-infected patients.

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“…Genetic variants of HCV in these populations may be due to diversity in H the race, routes of transmission and socioeconomic status (5,6). HCV genotypes have been shown to differ in their clinical outcome, prognosis, and response to antiviral therapy (7). HCV genotypes 1a and 4 may lead to chronic infection, more severe disease, re-infection, and poor response to therapy, compared to genotypes 2 and 3 (1).…”

Section: Introduction *mentioning

confidence: 99%

Distribution of Hepatitis C virus genotypes in Yazd, Central Province of Iran: increasing the mixed genotypes

et al. 2016

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Background and Aims: Different hepatitis C virus (HCV) genotypes have characteristic geographical distribution. Identification of HCV genotype is an important factor in the progression, clinical outcome and therapy of HCV infection. The aim of this study was to determine the prevalence of HCV genotypes among HCV-RNA positive patients in Yazd, Iran. Materials and Methods: In this cross-sectional study, 150 HCV-infected individuals with detectable plasma HCV RNA levels were enrolled from January to August 2015. HCV-RNA was extracted from plasma samples and retro-transcribed to c-DNA. Then HCV genotypes 1, 2, 3a, 4 were determined using a PCR based genotyping kit. Results: A total of 150 HCV-positive patients with mean age 40.45±11.83 were enrolled in the study. 89.3% of participant were males and 10.7% were females. The most common genotype was 3a (52%), followed by 1a (28%). Mixed-genotype infection was 20% and the most prevalent mixed genotype was 3a/1a (83% of mixed genotypes). The other genotypes were 1a/1b/3a in 10%, 3a/2 and 1a/2/3a both in 3% of patients with mixed HCV genotypes. Conclusions: Unlike other regions of Iran, Genotypes 3a was predominant in HCV-RNA positive patients in Yazd province. Also, HCV mixed-genotype infections were more common than previously estimated in other studies from different parts of the country.

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Mixed Genotypes in Hepatitis C Virus Infection

Cited by 3 publications

References 52 publications

Study of Hepatitis C Virus Infection at a Tertiary Hospital: Genotyping, Risk Factors and Comorbidities

Study of Hepatitis C Virus Infection at a Tertiary Hospital: Genotyping, Risk Factors and Comorbidities

Hepatitis C virus long‐term persistence in peripheral blood mononuclear cells in patients with haemophilia. Detection of occult genotype 1

Distribution of Hepatitis C virus genotypes in Yazd, Central Province of Iran: increasing the mixed genotypes

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