Mixed-mode chromatographic performance using nicotinic acid-functionalized chito-oligosaccharide-bonded Ti/Si hybrid monolithic capillary columns

El-Nouby, Mahmoud A. M.; Lim, Lee Wah

doi:10.1007/s44211-023-00416-3

Cited by 2 publications

(1 citation statement)

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“…The hydrophilic interaction liquid chromatography (HILIC) was coined by Alpert in 1990 and established as the ideal chromatographic model for polar and molecule separation . The HILIC retention mechanism principle can be explained as a partitioning mechanism between the aqueous layer associated with the polar stationary phase and the organic solvent content (40–98%) of the mobile phase. , Therefore, increasing the organic content of the mobile phase promotes a hydrophilic interaction, which magnifies the separation sensitivity. Furthermore, secondary interactions such as dipole–dipole, hydrogen bonding, and electrostatic interaction are possible in HILIC stationary phases .…”

Section: Resultsmentioning

confidence: 99%

Chitosan-Functionalized Methacrylate Monoliths for Mixed-Mode Hydrophilic/Electrostatic Interaction Capillary Liquid Chromatography

El-Nouby,

Anggraeni,

Kinoshita

et al. 2024

ACS Appl. Polym. Mater.

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Chitosan stands as a sustainable, active, and eco-friendly biomass that meets eight aspects of green chemistry principles. The chitosan-based liquid chromatography stationary phase provides superior physiochemical properties including a cationic hydrophilic surface, porosity, and mechanical strength; however, it lacks solubility in an organic solvent. A low-cost one-pot in situ free radical polymerization strategy was used to fabricate chitosan-functionalized methacrylate monolithic columns (Ch-GMA-co-EDMA) with hydrophilic and electrostatic properties. The polymerization mixtures were systematically optimized using mixture design to study the effects of compositions on flow-through processes involving porosity and permeability. The chitosan-functionalized methacrylate monomer and monolithic columns were characterized by using ATR-FTIR, TGA, DGA, and SEM. The obtained columns provide high chromatographic performance and satisfactory selectivity for organic polar, neutral, and charged compounds such as phenols, nucleic bases, nucleosides, and anionic pesticides. The results revealed that the amino and hydroxyl moieties of the chitosan monoliths functioned for both hydrophilic as well as electrostatic trapping of polar compounds, additionally promoting hydrogen bonding interaction with the analytes.

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