Thermal barrier coating (TBC) is an essential requirement of a modern gas turbine engine. The TBC failure is the delamination and spallation. The failure mechanism is interfacial expansion mismatch and oxidation of bond coat (BC). The oxidation damage under high temperature results in the reduction of interfacial adhesion. The interfacial fracture toughness is an important property to analyze the TBC failure. Using the simple tensile test, pushout test method and three-point or four-point-bending test and so on, the interfacial fracture toughness of ceramic top coat/BC has been researched in the past. However, the fracture toughness of the BC/substrate due to the Al depletion was very few studied. In this study, a NiCrAlY bond coat by air plasma spray (APS) was deposited. The substrate is directionally solidified superalloy (DZ40M). The Young's modulus of bond coat was obtained by the nanoindentation and average Young's modulus of bond coat is 66.9 GPa. Isothermal oxidation was performed at 1,050 • C for 100 h. Using the HXZ-1000 micro-hardness equipment and fracture mechanics approach, the five different times was chosen to test the hardness and the crack length, and then the fracture toughness was obtained. While the oxidation exposure time increased at 1,050 • C, the hardness of the substrate close to the bond coat decreased with the increase of the bond coat in hardness. Meanwhile, the interfacial fracture toughness of the bond coat-substrate decreased because of the Al depletion.