Mixed-monolayer glyconanoparticles for the detection of cholera toxin by surface enhanced Raman spectroscopy

Simpson, Jonathan; Craig, Derek; Faulds, Karen; Graham, Duncan

doi:10.1039/c5nh00036j

Cited by 18 publications

(22 citation statements)

References 30 publications

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“…Russell and co-workers used a thio-lactose (a disaccharide comprising lactose and a nonreducing terminal galactose) as the simplest molecular recognition element for cholera toxin stabilized onto Au NPs, thereby enabling the development of a colorimetric assay for cholera toxin detection. [112] A method with even higher sensitivity (28 × 10 −12 m) for cholera toxin detection was accomplished by Lee and coworkers, based on FRET between galactose-stabilized Au NPs and amine-terminated CdTe QDs. [111] Graham's group later sought the detection of cholera toxin, with greater sensitivity than previously achieved.…”

Section: Sensing Of Microorganismsmentioning

confidence: 99%

“…Upon aggregation of Ag NPs triggered by Cholera toxin, electromagnetic hot spots were generated, inducing an increase in RB1 SERS signal (Figure 6). [112] A method with even higher sensitivity (28 × 10 −12 m) for cholera toxin detection was accomplished by Lee and coworkers, based on FRET between galactose-stabilized Au NPs and amine-terminated CdTe QDs. In this case, the competitive assay between amino groups of QDs and Cholera toxin toward the selective binding to galactose on Au NPs caused FRET recovery, which was highly dependent on the amount of cholera toxin.…”

Section: Sensing Of Microorganismsmentioning

confidence: 99%

“…Reproduced under the term of the CC BY 3.0 license. [112] Copyright 2016, The Royal Society of Chemistry. www.advopticalmat.de negatively charged, nine-carbon monosaccharides, are usually in the terminal position of these glycan chains.…”

Section: Cell Phenotypingmentioning

confidence: 99%

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Plasmonic Detection of Carbohydrate‐Mediated Biological Events

Garcı́a

Mosquera

Plou

et al. 2018

Advanced Optical Materials

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regulation of a variety of physiological and pathological processes, such as proliferation and adhesion, immune response, and cell differentiation. [8] The biological roles of carbohydrates as signaling effectors and recognition markers are associated to specific molecular recognition events in which proteins [9] or other carbohydrates [10] are involved. However, individual carbohydrate-based molecular interactions have been shown to be generally weak and, therefore, biomolecules usually present clusters of carbohydrates that interact cooperatively with their natural ligands (multivalent effect), thereby increasing the binding strength. [11] Interestingly, the multivalent carbohydrate presentation can be mimicked by man-made multivalent systems, which involve multiple synthetic oligosaccharides, [12][13][14][15][16][17] and have been used to address basic studies, but also as probe molecules and drugs. Mammalian cells are covered by a dense array of carbohydrates known as glycocalyx. The glycocalyx composition depends on the specific cellular state, and therefore it can provide information about diseases. For example, an increase of α-2,6-sialylation, fucosylation, and/or other tumor-associated carbohydrate antigens on the cellular membrane is considered as a biomarker of cancer progression, with diagnostic value. [18,19] In addition, oligosaccharides can be used as disease biomarkers for targeted therapy, and therefore the analysis and characterization of the composition of cellular glycans provide information of relevance to the diagnosis of several diseases. [20] Different strategies can be used to label glycans, including the use of covalent recognizing ligands (e.g., boronic acid), using specific carbohydrate-binding proteins, and via metabolic labeling.Plasmonics is based on the excitation of surface plasmons, that is, coherent oscillations of electrons induced by an electromagnetic radiation at metal-dielectric interfaces. [21] When the dimensions of plasmonic nanostructures are smaller than the wavelength of the incident light, the interaction between the electromagnetic field and surface charges results in nonpropagating oscillations denoted as localized surface plasmon resonances (LSPR). [22] LSPR frequencies in metal nanoparticles depend on their size, shape, organization, interparticle distance, and dielectric environment, ranging from the visible through the near-infrared (NIR). [23,24] A large volume of research has been devoted to the development of reliable methods for the preparation of plasmonic nanostructures with well-defined shape, size, and/or interparticle spacing, as basic components of various analytical applications. [25,26] Another important element, the surface chemistry of plasmonic The incorporation of nanomaterials in glycoscience research enables the design of highly selective and sensitive bioanalytical devices for the detection of disease-associated biomarkers. In particular, localized surface plasmon resonance (LSPR) and surface enhanced Raman scattering (SERS) spectroscopies ar...

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Section: Sensing Of Microorganismsmentioning

confidence: 99%

Section: Sensing Of Microorganismsmentioning

confidence: 99%

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Plasmonic Detection of Carbohydrate‐Mediated Biological Events

Garcı́a

Mosquera

Plou

et al. 2018

Advanced Optical Materials

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“…They, thereby, jump in SERS intensity, without causing irreversible aggregation (Figure 3). The group has translated their "SERS-on" techniques for a variety of DNA, protein, and small molecule sensing applications, most recently by Mabbott et al for monitoring four fungal probes in a multiplexed fashion [157], by Simpson et al using a biomimetic glyconanoparticle assay for ultrasensitive (ng/ml) quantification of cholera toxin B-subunit [158], and by Gracie et al for the simultaneous detection of two meningitis bacterial DNA biomarkers extracted from cerebral spinal fluid (CSF) clinical samples [110,159].…”

Section: Molecularly Mediated Colloidal Sersmentioning

confidence: 99%

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

et al. 2017

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Point-of-care (POC) device development is a growing field that aims to develop low-cost, rapid, sensitive in-vitro diagnostic testing platforms that are portable, selfcontained, and can be used anywhere -from modern clinics to remote and low resource areas. In this review, surface enhanced Raman spectroscopy (SERS) is discussed as a solution to facilitating the translation of bioanalytical sensing to the POC. The potential for SERS to meet the widely accepted "ASSURED" (Affordable, Sensitive, Specific, Userfriendly, Rapid, Equipment-free, and Deliverable) criterion provided by the World Health Organization is discussed based on recent advances in SERS in vitro assay development. As SERS provides attractive characteristics for multiplexed sensing at low concentration limits with a high degree of specificity, it holds great promise for enhancing current efforts in rapid diagnostic testing. In outlining the progression of SERS techniques over the past years combined with recent developments in smart nanomaterials, high-throughput microfluidics, and low-cost paper diagnostics, an extensive number of new possibilities show potential for translating SERS biosensors to the POC.

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“…34 Silver nanoparticles functionalised with both sialic acid and galactose derivatives have been used in combination with extinction and surface enhanced Raman (SER) spectroscopies for the detection of cholera toxin. 35 A further advantage that the glyconanoparticles possess for the detection of Siglecs expressed on the surface of cells is their potential to overcome problems that other detection methods encounter including the presence of cis interactions and the low Siglecligand affinity. When interactions between Siglec and sialic acid are described, the initial assumption is that the Siglec of one cell binds glycans terminating in sialic acid that are on a second cell (in trans) and therefore, an intercellular adhesion takes place.…”

Section: Introductionmentioning

confidence: 99%

Detection of mSiglec-E, in solution and expressed on the surface of Chinese hamster ovary cells, using sialic acid functionalised gold nanoparticles

Schofield

Marín

Rejzek

et al. 2016

Analyst

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Sialic acids are widespread in biology, fulfilling a wide range of functions. Their cognate lectin receptors -Siglecs -are equally diverse and widely distributed, with different Siglecs found within distinct populations of cells in the haemopoietic, immune and nervous systems. A convenient way to assay ligand recognition of soluble Siglecs would be useful, as would methods for the concomitant assessment of Siglec distribution on cell surfaces. Here we report the use of gold nanoparticles functionalised with a sialic acid ligand diluted with a polyethylene glycol (PEG) ligand for the plasmonic detection of a soluble form of murine Siglec-E (mSiglec-E-Fc fusion protein) and, importantly, for the specific detection of the same siglec expressed on the surface of mammalian cells. These sialic acid functionalised nanoparticles are shown to overcome problems such as cellular cis interactions and low Siglec-ligand affinity. The gold nanoparticles were functionalised with various ratios of sialic acid:PEG ligands and the optimum ratio for the detection of murine Siglec-E was established based on the plasmonic detection of the soluble pre-complexed recombinant form of murine Siglec-E (mSiglec-E-Fc fusion protein). The optimum ratio for the detection of the fusion protein was found to be sialic acid:PEG ligands in a 50:50 ratio (glyconanoparticles 1).The optimised glyconanoparticles 1 were used to recognise and bind to the murine Siglec-E expressed on the surface of transfected Chinese hamster ovary cells as determined by transmission electron microscopy.3

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Mixed-monolayer glyconanoparticles for the detection of cholera toxin by surface enhanced Raman spectroscopy

Abstract: Cholera toxin B subunit (CTB) is detected sensitively in samples of synthetic freshwater by measuring changes in the SERS intensity of the glyconanoparticles upon their binding of CTB and subsequently aggregating. The particles are coated in both galactose and sialic acid.

Cited by 18 publications

References 30 publications

Plasmonic Detection of Carbohydrate‐Mediated Biological Events

Plasmonic Detection of Carbohydrate‐Mediated Biological Events

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

Detection of mSiglec-E, in solution and expressed on the surface of Chinese hamster ovary cells, using sialic acid functionalised gold nanoparticles

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