Mixed Polyelectrolyte Brush from Oppositely Charged Polymers for Switching of Surface Charge and Composition in Aqueous Environment

Houbenov, Nikolay; Minko, Sergiy; Stamm, Manfred

doi:10.1021/ma0341869

Cited by 162 publications

(222 citation statements)

References 31 publications

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“…8) suggests that the local chemical and electrostatic environment in the brush produces pK a shifts in some or all of the titratable residues, as observed in previous studies 35 . This pI-centred swelling behaviour has been previously observed for synthetic polyampholytes with charges randomly distributed along their length, which displayed at least threefold higher degree of swelling than mixed brushes of oppositely charged polyelectrolytes 36,37 . Recombinantly redistributing local charge density within rNFH-SA may offer additional opportunities to expand and tune these swelling dynamics 38 .…”

Section: Resultssupporting

confidence: 78%

Stimuli-sensitive intrinsically disordered protein brushes

et al. 2014

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Grafting polymers onto surfaces at high density to yield polymer brush coatings is a widely employed strategy to reduce biofouling and interfacial friction. These brushes almost universally feature synthetic polymers, which are often heterogeneous and do not readily allow incorporation of chemical functionalities at precise sites along the constituent chains. To complement these synthetic systems, we introduce a biomimetic, recombinant intrinsically disordered protein that can assemble into an environment-sensitive brush. This macromolecule adopts an extended conformation and can be grafted to solid supports to form oriented protein brushes that swell and collapse dramatically with changes in solution pH and ionic strength. We illustrate the value of sequence specificity by using proteases with mutually orthogonal recognition sites to modulate brush height in situ to predictable values. This study demonstrates that stimuli-responsive brushes can be fabricated from proteins and introduces them as a new class of smart biomaterial building blocks.

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Section: Resultssupporting

confidence: 78%

Stimuli-sensitive intrinsically disordered protein brushes

et al. 2014

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“…Neutral polymer brushes and polyelectrolyte brushes are widely used for the modification of surfaces, due to their advantageous specific properties, such as mechanical and chemical stability, their adsorption behavior [1] and permeability [2] and the possibility to tune their structure by varying external stimuli. For instance, the structure of a weak polyelectrolyte brush depends on pH [3][4][5][6][7][8], temperature [9][10][11], salt [12][13][14][15] and solvent [16][17][18]. More precisely, the swelling/shrinking of the studied poly-(N,N-(dimethylamino ethyl) methacrylate) (PDMAEMA) brushes can be manipulated by adjusting the pH of the surrounding environment to values below and above the pK A .…”

Section: Introductionmentioning

confidence: 99%

Stimuli-Responsive Polyelectrolyte Brushes As a Matrix for the Attachment of Gold Nanoparticles: The Effect of Brush Thickness on Particle Distribution

et al. 2014

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The effect of brush thickness on the loading of gold nanoparticles (AuNPs) within stimuli-responsive poly-(N,N-(dimethylamino ethyl) methacrylate) (PDMAEMA) polyelectrolyte brushes is reported. Atom transfer radical polymerization (ATRP) was used to grow polymer brushes via a "grafting from" approach. The brush thickness was tuned by varying the polymerization time. Using a new type of sealed reactor, thick brushes were synthesized. A systematic study was performed by varying a single parameter (brush thickness), while keeping all other parameters constant. AuNPs of 13 nm in diameter were attached by incubation. X-ray reflectivity, electron scanning microscopy and ellipsometry were used to study the particle loading, particle distribution and interpenetration of the particles within the brush matrix. A model for the structure of the brush/particle hybrids was derived. The particle number densities of attached AuNPs depend on the brush thickness, as do the optical properties of the hybrids. An increasing particle number density was found for increasing brush thickness, due to an increased surface roughness.

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“…35 Recently, we have found that the charge, thickness, and wettability of grafted polymer layers consisting of two oppositely charged polyelectrolytes can be switched by changing pH. 36,37 For example, a mixed polymer brush composed of two complementary polyelectrolytes, polyacrylic acid and poly͑2-vinyl pyridine͒ ͑PAA-mix-P2VP͒ is charged positively at low pH and negatively at high pH. Being a Brønsted base, monomer units of P2VP are protonated at low pH, charged positively, and the polymer chain is swollen.…”

Section: 27mentioning

confidence: 99%

“…36 The surface is weakly charged at the moderate pH range of 5.0-8.0. The character of switching depends on the ratio between the polyelectrolytes.…”

Section: 27mentioning

confidence: 99%

Stimuli-responsive command polymer surface for generation of protein gradients

et al. 2009

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Mixed polyelectrolyte brushes with a composition gradient were used as a platform for fabrication of stimuli-responsive command surfaces to control the generation of concentration gradients of adsorbed protein molecules. Switching between homogeneously adsorbed protein layers and adsorbed layers with protein concentration gradients was achieved by changing the pH of protein aqueous solutions. Protein adsorption and the direction of the adsorption gradient were tuned and also turned off and on or reversed by tuning the proton concentration in the pH range 4.0–8.6.

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Mixed Polyelectrolyte Brush from Oppositely Charged Polymers for Switching of Surface Charge and Composition in Aqueous Environment

Cited by 162 publications

References 31 publications

Stimuli-sensitive intrinsically disordered protein brushes

Stimuli-sensitive intrinsically disordered protein brushes

Stimuli-Responsive Polyelectrolyte Brushes As a Matrix for the Attachment of Gold Nanoparticles: The Effect of Brush Thickness on Particle Distribution

Stimuli-responsive command polymer surface for generation of protein gradients

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