Mixed‐Up Sugars: Glycosyltransferase Cross‐Reactivity in Cancerous Tissues and Their Therapeutic Targeting

Cid, Emili; Yamamoto, Miyako; Yamamoto, Fumiichiro

doi:10.1002/cbic.202100460

Cited by 3 publications

(2 citation statements)

References 82 publications

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“…Humans are considered a largely Forssman-negative species and the presence of Forssman antigenicity is mostly associated with human cancer 69 . During the last decade, the elucidation of the genetic basis behind FA reactivation and the establishment of the human FORS blood group underline the continual relevance of Forssman antigen 5,6 .…”

Section: Discussionmentioning

confidence: 99%

A novel and highly specific Forssman antigen-binding protein from sheep polyomavirus

Rustmeier

Silva

Maio

et al. 2023

Preprint

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Polyomaviruses are small, non-enveloped double-stranded DNA viruses of humans and other mammals, birds, and fish. Infections are usually asymptomatic and result in latency, however, some polyomaviruses can induce severe diseases, including cancer, in immunocompromised individuals. Established cellular receptors for polyomavirus infection are sialylated glycolipids (such as gangliosides), membrane proteins, and glycosaminoglycans. Polyomaviruses are usually highly host specific, but the exact principles that govern host tropism remain unknown in many cases. Here, glycan array screening shows that the major capsid protein VP1 of sheep polyomavirus (ShPyV) binds to the Forssman glycolipid, an antigen of many vertebrates and a potential tumor marker in humans. Following closer investigation, we can report for the first time that a neutral, non-sialylated glycolipid acts as a polyomavirus receptor. Concurrently, we present the first report of a viral protein that specifically engages the Forssman antigen. We demonstrate that ShPyV VP1 binds to Forssman-positive erythrocytes but not those of human A, B and O blood groups, which is a clear distinction from features thus far described for Forssman lectins. X-ray crystallography and structural analysis of the VP1-Forssman glycan complex define the terminal Forssman disaccharide as the determinant of this protein-receptor interaction. These results strongly suggest that the sheep polyomavirus can use Forssman antigen for infectious cell entry. Furthermore, the ability of ShPyV VP1 to distinguish Forssman-positive from -negative cells may prove useful for monitoring the Forssman-'status' of normal, preneoplastic and neoplastic cells and tissues and establishing the antigen level as a biomarker.

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Section: Discussionmentioning

confidence: 99%

A novel and highly specific Forssman antigen-binding protein from sheep polyomavirus

Rustmeier

Silva

Maio

et al. 2023

Preprint

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“…Chen's group also summarized the development and application of glycosyltransferases until 2021 [53]. A few new glycosyltransferases [66][67][68] relevant to cell labeling have been reported in the last two years, with more attention being paid to improving performance [69] and expanding substrates [70] and applications [71][72][73][74][75][76]. The technique faces several challenges: glycosyltransferases that can tolerate unnatural monosaccharide donors are difficult to find; unnatural monosaccharide derivatives are more difficult to synthesize than the unnatural sugars of MGL; and chemoenzymatic labeling techniques are only suitable for reporting steady-state glycosylation and not for monitoring dynamic glycosylation.…”

Section: Chemoenzymatic Glycan Labeling (Cegl)mentioning

confidence: 99%

Cell-Surface Glycan Labeling and Sensing

Li,

Wang,

Ding

et al. 2023

Targets

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Cell-surface glycans are abundant and complex and play a critical role in maintaining protein stability, regulating cell behavior, and participating in cell communication. Obtaining structural information on glycans in situ is helpful to further understand the role of glycans in the physiological and pathological processes of cells and the regulatory mechanism. To achieve this, we can use recognition or labeling strategies to convert the presence of glycans on the cell surface into signals that can be detected. Currently, many different types of in situ sensing strategies for glycans have been developed. The spatial control of the conversion process can realize the restriction of glycan detection to specific proteins, and the introduction of signal amplification technology into the conversion process can improve the sensitivity of sensing. In this paper, the recent progress of glycan labeling methods and sensing technology is reviewed, and the future development direction is prospected.

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Galactia lindenii lectin type-II. Proposal of its potential use in diagnostic tools

Cortázar,

Vega,

Reyes-Montaño

et al. 2024

Preprint

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Galactia lindenii lectin type-II (GLL-II) belongs to the group of the legume lectins. The present study investigated the GLL-II staining patterns in histological sections of neoplastic and non-neoplastic thyroid tissues. Besides, hemagglutination assays (HA) using the GLL-II on red blood cells (RBCs) of different glycomic profile were performed, complementing previous results. The differential staining in Papillary Thyroid Cancer (PTC), Invasive Encapsulated Follicular Variant Papillary Thyroid Carcinoma (IEFV-PTC), Hashimoto's thyroiditis (HT), and non-neoplastic thyroid with goiter changes, together with the HA results and along with reviewed glycoprofiles of unhealthy conditions in other organs, allowed us to propose the potential utility of GLL-II in lectin platforms used to discriminate human pathological samples from normal ones. The present study shed light on potential applications of GLL-II in determining alterations of glycosylation patterns in specific cells, tissues, or body fluids, as well as glycotopes biomarkers of healthy or pathological conditions.

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Mixed‐Up Sugars: Glycosyltransferase Cross‐Reactivity in Cancerous Tissues and Their Therapeutic Targeting

Cited by 3 publications

References 82 publications

A novel and highly specific Forssman antigen-binding protein from sheep polyomavirus

A novel and highly specific Forssman antigen-binding protein from sheep polyomavirus

Cell-Surface Glycan Labeling and Sensing

Galactia lindenii lectin type-II. Proposal of its potential use in diagnostic tools

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