A tuning method was proposed for automatic lighting (auto-lighting) algorithms derived from the steepest descent and conjugate gradient methods. The auto-lighting algorithms maximize the image quality of industrial machine vision by adjusting multiple-color light emitting diodes (LEDs)-usually called color mixers. Searching for the driving condition for achieving maximum sharpness influences image quality. In most inspection systems, a single-color light source is used, and an equal step search (ESS) is employed to determine the maximum image quality. However, in the case of multiple color LEDs, the number of iterations becomes large, which is time-consuming. Hence, the steepest descent (STD) and conjugate gradient methods (CJG) were applied to reduce the searching time for achieving maximum image quality. The relationship between lighting and image quality is multi-dimensional, non-linear, and difficult to describe using mathematical equations. Hence, the Taguchi method is actually the only method that can determine the parameters of auto-lighting algorithms. The algorithm parameters were determined using orthogonal arrays, and the candidate parameters were selected by increasing the sharpness and decreasing the iterations of the algorithm, which were dependent on the searching time. The contribution of parameters was investigated using ANOVA. After conducting retests using the selected parameters, the image quality was almost the same as that in the best-case parameters with a smaller number of iterations.